标题:Metagenomic Analysis of Rhizospheric Bacterial Community of Citrus Trees Expressing Phloem-Directed Antimicrobials

作者: Nunez-Munoz, Leandro Alberto, Sanchez-Garcia, Martin Eduardo, Calderon-Perez, Berenice, De la Torre-Almaraz, Rodolfo, Ruiz-Medrano, Roberto and Xocon

摘要:Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tIssues. This study investigated the effects of genetically modified citrus trees expressing Citrus sinensis PP16 (CsPP16) fused to human lysozyme and beta-defensin-2 on the soil microbiome diversity using 16S amplicon analysis. The results indicated that there were no significant alterations in alpha diversity, beta diversity, phylogenetic diversity, differential abundance, or functional prediction between the antimicrobial phloem-overexpressing plants and the control group, suggesting minimal impact on microbial community structure. However, microbiota diversity analysis revealed distinct bacterial assemblages between the rhizosphere soil and root environments. This study helps to understand the ecological implications of crops expressing phloem-targeted antimicrobials for vascular disease management, with minimal impact on soil microbiota.

文献注录: Nunez-Munoz, Leandro Alberto, Sanchez-Garcia, Martin Eduardo, Calderon-Perez, Berenice, De la Torre-Almaraz, Rodolfo, Ruiz-Medrano, Roberto and Xocon. Metagenomic Analysis of Rhizospheric Bacterial Community of Citrus Trees Expressing Phloem-Directed Antimicrobials [J]. . 2024.

报/刊名:》,发表于2024

文献类型: [J]

页码: 页 / 共

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