作者：Su, Hang, Wang, Yuanchun, Xu, Jin, Omar, Ahmad A., Grosser, Jude W., Calovic, Milica, Zhang, Liyang, Feng, Yu, Vakulskas, Christopher A. and Wang, Nia
摘要：Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars is the most effective, environmentally friendly and economic approach for disease control. However, citrus traditional breeding is lengthy and laborious. Here, we develop transgene-free canker-resistant Citrus sinensis lines in the T0 generation within 10 months through transformation of embryogenic protoplasts with Cas12a/crRNA ribonucleoprotein to edit the canker susceptibility gene CsLOB1. Among the 39 regenerated lines, 38 are biallelic/homozygous mutants, demonstrating a 97.4% biallelic/homozygous mutation rate. No off-target mutations are detected in the edited lines. Canker resistance of the cslob1-edited lines results from both abolishing canker symptoms and inhibiting Xcc growth. The transgene-free canker-resistant C. sinensis lines have received regulatory approval by USDA APHIS and are exempted from EPA regulation. This study provides a sustainable and efficient citrus canker control solution and presents an efficient transgene-free genome-editing strategy for citrus and other crops. Development of canker-resistant citrus cultivars via traditional approaches is a lengthy and laborious process. Here, the Authors report the generation of regulatory approval, transgene-free, canker-resistant sweet orange lines using Cas12a/crRNA ribonucleoprotein-based susceptibility gene editing strategy.
文献注录：Su, Hang, Wang, Yuanchun, Xu, Jin, Omar, Ahmad A., Grosser, Jude W., Calovic, Milica, Zhang, Liyang, Feng, Yu, Vakulskas, Christopher A. and Wang, Nia. Generation of the transgene-free canker-resistant Citrus sinensisusing Cas12a/crRNA ribonucleoprotein in the T0 generation [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
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