作者：Zhang Li, Xu Yang, Li Yanting,Zheng Saisai,Zhao Zhenmei,Chen Meiling,Yang Haijian,Yi Hualin,Wu Juxun
摘要：MYB family transcription factors play essential roles in various biological processes, yet their involvement in regulating fruit ripening and fruit size in citrus remains poorly understood. In this study, we have established that the R2R3-MYB transcription factor, CsMYB77, exerts a negative regulatory influence on fruit ripening in both citrus and tomato (Solanum lycopersicum), while also playing a role in modulating fruit size in citrus. The overexpression of CsMYB77 in tomato and Hongkong kumquat (Fortunella hindsii) led to notably delayed fruit ripening phenotypes. Moreover, the fruit size of Hongkong kumquat transgenic lines was largely reduced. Based on DNA affinity purification sequencing (DAP-Seq) and verified interaction assays, SEVEN IN ABSENTIA OF ARABIDOPSIS THALIANA4 (SINAT4) and PIN-FORMED PROTEIN5 (PIN5) were identified as downstream target genes of CsMYB77. CsMYB77 inhibited the expression of SINAT4 to modulate abscisic acid (ABA) signalling, which delayed fruit ripening in transgenic tomato and Hongkong kumquat lines. Expression of PIN5 was activated by CsMYB77, which promoted free IAA decline and modulated auxin signalling in the fruits of transgenic Hongkong kumquat lines. Taken together, our findings revealed a fruit development and ripening regulation module (MYB77-SINAT4/PIN5-ABA/auxin) in citrus, which enriches the understanding of the molecular regulatory network underlying fruit ripening and size.
文献注录：Zhang Li, Xu Yang, Li Yanting,Zheng Saisai,Zhao Zhenmei,Chen Meiling,Yang Haijian,Yi Hualin,Wu Juxun. Transcription factor CsMYB77 negatively regulates fruit ripening and fruit size in citrus [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
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