作者：Irigoyen, S., Ramasamy, M., Pant, S., Niraula, P., Bedre, R., Gurung, M., Rossi, D., Laughlin, C., Gorman, Z., Achor, D., Levy, A., Kolomiets, M. V.,
摘要：A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibacter spp., the presumptive causal agents of citrus greening, potato zebra chip and tomato vein greening diseases. Importantly, we leverage the microbial hairy roots for rapid, reproducible efficacy screening of multiple therapies. We identify six antimicrobial peptides, two plant immune regulators and eight chemicals which inhibit Candidatus Liberibacter spp. in plant tIssues. The antimicrobials, either singly or in combination, can be used as near- and long-term therapies to control citrus greening, potato zebra chip and tomato vein greening diseases. The putative causal agent of citrus greening Candidatus Liberibacter asiaticus (CLas) cannot be cultured, which hampers finding new therapies to control this devastating disease. Here, the Authors show that hairy roots support CLas propagation and enable high throughput antimicrobial screening.
文献注录：Irigoyen, S., Ramasamy, M., Pant, S., Niraula, P., Bedre, R., Gurung, M., Rossi, D., Laughlin, C., Gorman, Z., Achor, D., Levy, A., Kolomiets, M. V., . Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp [J]. . 2020.
报/刊名：《》，发表于2020
文献类型： [J]
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