作者：Darolt, J. C., Raiol, L. L., Jr., Carvalho, E. V., Andrade, L. L., Oliveira, V. S. L., Gomes, P. A., Alves, M. N., Moreira, A. S., Volpe, H. X. L., Ci
摘要：Genetic resistance to 'Candidatus Liberibacter asiaticus' (CLas) is considered a promising long-term solution to control Huanglongbing (HLB), given that all commercial citrus varieties are susceptible to the disease. Only a few citrus relatives have been reported as putatively CLas resistant; however, these were only evaluated as scion varieties. Here, we assessed CLas-induced damages and titer in scion/rootstock combinations, including Citrus and certain putatively CLas-resistant relatives that were previously selected. In experiment I, 'Valencia' sweet orange (VSO) was grafted onto either BGC682 hybrid (a complex admixture of Australian citrus species) or 'Rangpur' lime (RL) as a control, and CLas was inoculated via bud grafting onto the scion. CLas was detected in most of VSO/RL and VSO/BGC682 scions, but it remained undetectable in BGC682 roots, while all RL tissues were CLas positive. In experiments II, III and IV, putatively CLas-resistant Clausena lansium (Clu) was used as the scion, rootstock or interstock. When inoculated by top-grafting, CLas was detected in most tissues from the control groups (only citrus stocks). No bacterium was detected in Citrus trifoliata (Ctr)/Clu and RL/Clu roots. Upon Asian citrus psyllid (ACP) inoculation, CLas infection levels were reduced, and ACP mortality rates were elevated in Clu-grafted treatments compared to the control (VSO/RL), likely attributed to nutrient deficiencies and slower development rates. Under field conditions with natural ACP-mediated CLas transmission, citrus controls were 100% infected with CLas. In contrast, Clu and Swinglea glutinosa (Swg) treatments had lower HLB incidence, but only Swg-grafted trees resulted in satisfactory plant growth compared to Clu-grafted trees. In summary, our findings suggest that Clu and BGC682, when used as citrus rootstocks, exhibit putative resistance to CLas. Although their practical application remains limited by graft incompatibility for Clu and high susceptibility to citrus tristeza virus of BGC682, further biotechnological and classic approaches can be used to incorporate their resistance into citrus breeding programmes.
文献注录：Darolt, J. C., Raiol, L. L., Jr., Carvalho, E. V., Andrade, L. L., Oliveira, V. S. L., Gomes, P. A., Alves, M. N., Moreira, A. S., Volpe, H. X. L., Ci. 'Candidatus Liberibacter asiaticus colonization in citrus trees grafted onto rootstocks of citrus relatives ' [J]. . 2025.
报/刊名：《》，发表于2025
文献类型： [J]
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