作者：Kelly, Gilor, Plesser, Elena, Bdolach, Eyal, Arroyave, Maria, Belausov, Eduard, Doron‐\Faigenboim, Adi, Rozen, Ada, Zemach, Hanita, Zach, Yair Yehoshu
摘要：Recent advances in genome editing offer transformative potential for fruit tree breeding, yet conventional methods relying on tissue culture remain laborious and prone to somaclonal variation. Here, we present *in planta* genome editing in citrus (IPGEC), a novel Agrobacterium-mediated system enabling direct genome editing in soil-grown seedlings without tissue culture. IPGEC integrates transient co-expression of three functional modules: (1) CRISPR/Cas9 machinery targeting *CsPDS* (a carotenoid biosynthesis gene), (2) developmental regulators (e.g., *WUS*, *STM*, *IPT*) to enhance *de novo* shoot regeneration, and (3) T-DNA delivery boosters (VIP1/VirE2) under a cell division-specific promoter (YAO). This approach achieved up to 75% regeneration efficiency in Carrizo citrange, with multiplex editing confirmed by albino phenotypes and sequencing. Notably, high-throughput screening identified transgene-free chimeric or fully edited shoots (e.g., line #81), demonstrating IPGEC’s potential for non-GMO breeding. Developmental regulator efficacy varied across cultivars (Carrizo, Duncan, Foster, Hudson), with IPT or GRF4-GIF1 optimizing transformation rates (12.5–30%). By bypassing tissue culture and enabling transient editing, IPGEC accelerates trait-specific improvements in elite citrus varieties while mitigating somaclonal risks. This system establishes a framework for precision breeding in perennial crops, addressing challenges of heterozygosity and long generation cycles in fruit trees.
文献注录：Kelly, Gilor, Plesser, Elena, Bdolach, Eyal, Arroyave, Maria, Belausov, Eduard, Doron‐\Faigenboim, Adi, Rozen, Ada, Zemach, Hanita, Zach, Yair Yehoshu. In planta genome editing in citrus facilitated by co‐\expression of CRISPR/Cas and developmental regulators [J]. . 2025.
报/刊名：《》，发表于2025
文献类型： [J]
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