作者：Lan, Moying, Li, Kaijiang, Luo, Cong, Li, Yuze, Liu, Yuan, Nai, Yi, Hu, Wanli, Huang, Guixiang and He, Xinhua
摘要：The plant elongation factor eEF1A is involved in coregulating not only the translation of proteins and controlling translation-related signaling but also in signaling associated with cell growth, stress response and motility, controlling apoptosis and responding to adversity in plants. In this study, four eEF1A genes, namely, ClEF1A-1, ClEF1A-2, ClEF1A-3 and ClEF1A-4, were identified from the genomic and ubiquitin-modified omics data of the 'Xiangshui Lemon', and bioinformatics analysis revealed that these four genes have relatively similar structures with conserved sequences; ClEF1A-1 and ClEF1A-4 were highly expressed in pollen, and temporal expression analysis demonstrated that the expression of ClEF1As was significantly greater under self-pollination than under cross-pollination. All four genes were localized in the nucleus. ClEF1As overexpression promoted early flowering and improved drought and salt stress tolerance in transgenic Arabidopsis plants. Yeast two-hybrid assays revealed that ClEF1As interacted with F-box, eIF3-G, the organ-specific-like protein S2, AGL62, S1-RNase, S2-RNase, S3- RNase and S4-RNase. This study demonstrated the functions of ClEF1As and provided a baseline for further studies on the associations of ClEF1As with self-incompatibility and abiotic stresses.
文献注录：Lan, Moying, Li, Kaijiang, Luo, Cong, Li, Yuze, Liu, Yuan, Nai, Yi, Hu, Wanli, Huang, Guixiang and He, Xinhua. Four ClEF1A genes involved in self-incompatibility in 'Xiangshui Lemon' confer early fowering and increase stress tolerance in transgenic Arabidopsis [J]. . 2024.
报/刊名：《》，发表于2024
文献类型： [J]
页码：第 页 / 共 页