作者：Wang, S., Zhao, S. L., Du, M. X., Mo, K. Q., Pang, S. W. and Zou, X. P.
摘要：Conventional breeding techniques have been effectively utilized for the enhancement of citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross- or self-incompatibility, high genetic heterozygosity, and polyembryony have posed significant challenges and limitations to these methods. The clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has progressively emerged as a vital tool for citrus breeding and research. This article reviews the array of CRISPR/Cas genome editing systems, emphasizes recent advancements in citrus genome editing using CRISPR/Cas, and explores the application of this technology to bolster resistance to citrus canker. The review also covers the development of CRISPR/Cas-mediated transformation and regeneration systems for citrus, alongside approaches for generating transgene-free citrus germplasm. Moreover, the regulatory landscape and societal acceptance of CRISPR/Cas genome editing are examined. Lastly, potential applications of genome editing in citrus breeding are proposed, with attention to prospective challenges.
文献注录：Wang, S., Zhao, S. L., Du, M. X., Mo, K. Q., Pang, S. W. and Zou, X. P.. Development and application of gene editing in citrus: how and what to do [J]. . 2024.
报/刊名：《》，发表于2024
文献类型： [J]
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