作者：Zhao, P., Sun, Y., Chen, X., Zhang, J., Yang, H., Hao, X., Fang, R. and Ye, J.
摘要：The spread of insect-borne plant pathogens depends on their ability to manipulate the behavior of their vectors. Some bacteria-infected plants become more attractive to vectors, but the underlying mechanism remains poorly understood. Huanglongbing (HLB), a devastating citrus disease, is primarily caused by the bacterium 'Candidatus Liberibacter asiaticus' (CLas) and is transmitted by psyllid vectors. In this study, we identify that the effector protein SDE5, secreted by CLas, inhibits the biosynthesis of volatile terpenoids in host citrus plants, thereby enhancing the attraction of psyllid vectors. Biochemically, SDE5 acts as an inhibitor of bacterial C-type lysozyme, promoting both CLas infection and psyllid vector attraction. Two plant U-box (PUB) E3 ligases, PUB10 and PUB21, could be recruited by SDE5 to promote ubiquitination and proteasomal degradation of MYC2, the key transcription factor in jasmonate signaling and terpene-based anti-herbivore defenses. Additionally, SDE5 disrupts MYC2 dimerization, effectively attenuating its capacity to activate terpene biosynthesis genes. This dual suppression function significantly reduces volatile terpenoid emissions in SDE5-transgenic citrus lines, resulting in heightened psyllid attraction and enhanced psyllid fitness. Conversely, the anti-proteolysis peptide 3-14 (APP 3-14), which stabilizes MYC2 protein and inhibits the HLB pathogen, increases the emission of volatile terpenoids to repel psyllids. These findings provide a novel strategy for disrupting mutualistic alliances between plant bacterial pathogens and insect vectors through manipulating olfactory defense.
文献注录：Zhao, P., Sun, Y., Chen, X., Zhang, J., Yang, H., Hao, X., Fang, R. and Ye, J.. A small peptide APP3-14 disrupts pathogen-insect mutualism via modulating plant MYC2-mediated olfactory defense [J]. . 2025.
报/刊名：《》，发表于2025
文献类型： [J]
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