作者：易龙,陶珍珍,卢占军,钟八莲
摘要：运用RT-PCR技术对柑桔裂皮类病毒江西分离物CEVd-RJ的全长cDNA序列进行扩增、克隆,并对其片段进行序列测定。将获得的371bp的全长cDNA序列与GenBank登录的11个柑桔裂皮类病毒相应序列进行比对分析,结果发现CEVd-RJ与湖北分离物CEVd-HB的同源性为99.7%,与广东分离物CEVd-YC的同源性为90.8%,同国外9个分离物间的同源性在89.0%～98.6%之间。序列差异分析发现,CEVd-RJ的全长cDNA序列比CEVd-YC少一个碱基,有36个位点碱基发生了变化,与CEVd-HB只存在一个碱基位点的差异。
The Citrus exocortis viroid,CEVd-RJ,isolated from Jiangxi province was amplified using RT-PCR,and the full length cDNA was cloned and sequenced.Comparison with the 11 citrus exocortis viroid isolates deposited in GenBank revealed that the Jiangxi isolate,CEVd-RJ,shared 99.7% of identity with the Hubei isolate,CEVd-HB,and 90.8% of identity with the Guangdong isolate,CEVd-YC,in their cDNA sequences.CEVd-RJ also shared 89.0% to 98.6% cDNA identities with 9 isolates from other countries.Compared with CEVd-YC,CEVd-RJ had a 1 bp deletion and 36 nucleotide changes in its full length cDNA.Notably,there was only one nucleotide difference between CEVd-RJ and CEVd-HB.
关键词：柑桔裂皮类病毒; 江西; 全长cDNA; 序列分析; Citrus exocortis viroid; Jiangxi; full length of cDNA
文献注录：易龙,陶珍珍,卢占军,钟八莲. 柑桔裂皮类病毒江西分离物（CEVd-RJ）全长cDNA序列分析 [J]. 中国南方果树. 2012, 06: 1-4.
报/刊名：《中国南方果树》，发表于2012 / 第 06 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 1-4 页 / 共4 页