作者：Rosely Pereira da Silva, Maria Angélica Pereira de Carvalho Costa, Antonio da Silva Souza, Weliton Antonio Bastos de Almeida.
摘要：The objective of this work was to establish an efficient protocol for in vitro regeneration of plants through organogenesis of sweet orange ‘Pera’ (Citrus sinensis L. Osbeck) juvenile explants, in order to give support for future studies of genetic transformation. Epicotyl segments used as explants were introduced in MT culture media. To maximize the regeneration of in vitro plants, experiments were performed to evaluate different concentrations of BAP in the culture media (0, 1, 2, 3 or 4 mg L-1), size (0.25, 0.5 or 1 cm), polarity (basal, medium, and apical), position (horizontal or vertical), luminosity conditions (photoperiod of 16 hours, and darkness for 30 days), and incision of the segment ends. In addition, better conditions for obtaining rooted plants (culture media MT, MT/2, with and without auxin and micrografting) were studied. The combination of 3 mg L-1 BAP with segments of 0.25 cm length was efficient for the organogenetic response. Segment apices and mediums were more efficient comparing to the basal parts. Culturing the segments in the horizontal position, with a photoperiod of 16 hours, was more efficient than in the vertical position. There was no improvement in the induction of in vitro organogenesis, when the explant ends were incised. Micrografting assured 100% of rooted shoots.
关键词：Citrus sinensis, plant tissue culture, juvenile explants, benzilaminopurine, mic
文献注录：Rosely Pereira da Silva, Maria Angélica Pereira de Carvalho Costa, Antonio da Silva Souza, Weliton Antonio Bastos de Almeida.. Regeneration of ‘Pera’ sweet orange plants through in vitro organogenesis [J]. Pesquisa Agropecuária Brasileira. 2005, 12: 1153-1159.
报/刊名：《Pesquisa Agropecuária Brasileira》，发表于2005 / 第 12 期。
文献类型： [J]
页码：第 1153-1159 页 / 共7 页