作者：Xu, X. Y.,Liu, J. H.,Deng, X. X.
作者单位：1National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University
摘要：Oleander is a Mediterranean evergreen shrub found along watercourses, gravelly places and damp slopes. It is
grown widely as an ornamental for its abundant and long-lasting flowering as well as its moderate hardiness.
Genetic relatedness among 71 accessions, including commercial varieties, different sources of the same varieties,
and selections from the wild were investigated using amplified fragment length polymorphism (AFLP). Nine
primer combinations yielded a total of 603 bands of which 241 were polymorphic. Genetic similarities among
accessions were calculated according to Jaccard’s Similarity Index and used to construct a dendrogram based on
the unweighted pair group method using arithmetic averages. Our results show that the AFLP technique, which
can simultaneously and assay a large number of loci randomly distributed in the genome, is much more inform-ative on the genetic relationship and origin of accessions than the limited number of morphological characters
conventionally used for variety discrimination. Up to about 9% molecular genetic differentiation was detected
among morphologically indistinguishable provenances of the same variety; this can be partly attributed to scoring
error but mainly to somatic variation occurring during vegetative propagation. On the other hand lower genetic
distance values were sometimes found among varieties which differ in morphological characters and are thus
commercialised with different names. The possibility of considering the amount of genetic variation within a
variety as the threshold value for discrimination of initial varieties and essentially derived varieties is discussed
关键词：CAPS,Citrus,cpDNA,cybrids,flow cytometry, Microcitrus, mtDNA, SSR, somatic hy
文献注录：Xu, X. Y.,Liu, J. H.,Deng, X. X.. Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium) [J]. Euphytica. 2004, 02: 115-123.
报/刊名：《Euphytica》，发表于2004 / 第 02 期。
文献类型： [J]
页码：第 115-123 页 / 共8 页