作者:Moreira, C. D.,Chase, C. D.,Gmitter, F. G.,Grosser, J. W.
作者单位:Institute of Food and Agricultural Sciences, Horticultural Sciences Dept., University of Florida,Institute of Food and Agricultural Sciences, CREC, University of Florida
摘要:Restriction fragment length polymorphisms (RFLPs), were used to analyze the organelle composition of two-year-old trees, recovered from two different experiments: protoplasts from embryogenic cell suspensions of `Succari' sweet orange (C. sinensis L. Osbeck) were fused with leaf protoplasts of Citropsis gilletiana Swingle & M. Kell or to leaf protoplasts of Atalantia ceylanica (Arn.) Oliv. The somatic hybrids of both fusion combinations had the mitochondrial genome from the embryogenic partner. In some somatic hybrids, non-parental fragments were observed among the mitochondrial patterns. Somatic hybrids between `Succari' + Atalantia had plastid DNA from the embryogenic parent, while the somatic hybrids of `Succari' + Citropsis all had both parental chloroplast genomes. The relative abundance of organelle DNAs in the donor embryogenic and leaf cells may explain the consistent transmission of the embryogenic parent mitochondrial DNA and the inheritance of the chloroplast genome from either parent.
关键词:chloroplast DNA,citrus,mitochondrial DNA,RFLPs,somatic hybrids,mitochondrial gen
文献注录:Moreira, C. D.,Chase, C. D.,Gmitter, F. G.,Grosser, J. W.. Transmission of organelle genomes in citrus somatic hybrids [J]. Plant Cell Tissue and Organ Culture. 2000, 02: 165-168.
报/刊名:《Plant Cell Tissue and Organ Culture》,发表于2000 / 第 02 期。
文献类型: [J]
页码:第 165-168 页 / 共4 页