作者：SD Singer，KD Cox
摘要：Membrane-localized H + -symporting sucrose transporters (SUC or SUT proteins) are involved in sucrose loading into the phloem of source tissues and sucrose uptake into sink tissues, which are essential events in the growth and development of higher plants. While many of these sucrose transporters are localized in the phloem, others function in sink tissues. In an attempt to gain insight into which class the CsSUT1 gene from Citrus sinensis falls, we isolated a 1537-bp upstream region of this gene ( CsSUT1p ), inserted it upstream of the β-glucuronidase ( GUS ) reporter gene and transformed the resulting vector into Arabidopsis thaliana . Histochemical and semi-quantitative RT-PCR analyses indicated that the CsSUT1p conferred GUS expression in floral tissues and the roots of young seedlings, but not above ground vegetative tissues. In flowers, GUS expression was noted in young floral buds, as well as immature stamens and carpels. Deletion analyses indicated that a −1052 to −1 fragment (relative to the translational start codon at +1) of the CsSUT1p , but not a −496 to −1 fragment, was able to drive the same pattern of expression of a downstream reporter gene in transgenic Arabidopsis . Taken together, these results suggest that the CsSUT1 gene, like numerous SUC/SUT genes from other plant species, may play a role in the uptake of sucrose into sink tissues.
关键词：Sucrose transporter , CsSUT1 promoter ,Citrus sinensis ,Arabidopsis thaliana ,β-
文献注录：SD Singer，KD Cox. The CsSUT1 promoter from Citrus sinensis confers sink-specific expression of a downstream reporter gene in transgenic Arabidopsis [J]. Journal of Plant Biochemistry and Biotechnology. 2012, 21（2）: 167-172.
报/刊名：《Journal of Plant Biochemistry and Biotechnology》，发表于2012 / 第 21（2） 期。
文献类型： [J]
页码：第 167-172 页 / 共6 页