作者：M Cervera，J Juárez，L Navarro，L Peña
摘要：Abstract Most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. Evaluation of characteristics related to fruits is a requisite to release any new variety into the market. Because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. Direct transformation of mature material could ensure the production of adult transgenic plants, bypassing in this way the juvenile phase. Invigoration of the source adult material, establishment of adequate transformation and regeneration conditions, and acceleration of plant development through grafting allowed us to produce transgenic mature sweet orange trees flowering and bearing fruits in a short time period.
关键词：Adult plants , cell competence , genetic engineering ,grafting , invigoration ,
文献注录：M Cervera，J Juárez，L Navarro，L Peña. Genetic transformation of mature citrus plants [J]. Methods in Molecular Biology. 2005, 286(286): 177-187.
报/刊名：《Methods in Molecular Biology》，发表于2005 / 第 286(286) 期。
文献类型： [J]
页码：第 177-187 页 / 共11 页