作者：Ben Mahmoud, Sulley Ramos, John E. Shatters, Robert G., Jr. Hall, David G. Lapointe, Stephen L. Niedz, Randall P. Rouge, Pierre Cave, Ronald D. Borovs
作者单位： United States Horticultural Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 2001 South Rock Road, Ft. Pierce, FL 34945, USA
摘要：Diaprepes abbreviatus (L) is an important pest of citrus in the USA. Currently, no effective management strategies of D. abbreviatus exist in citriculture, and new methods of control are desperately sought. To protect citrus against D. abbreviatus a transgenic citrus rootstock expressing Bacillus thuringiensis Cyt2Cal, an insect toxin protein, was developed using Agrobacterium-mediated transformation of \'Carrizo\' citrange [Citrus sinensis (L) Osbeck Poncirus trifoliate (L) Rafl. The transgenic citrus root stock expressed the cytolytic toxin Cyt2Ca1 constitutively under the control of a 35S promoter in the transgenic Carrizo citrange trifoliate hybrid including the roots that are the food source of larval D. abbreviatus. The engineered citrus was screened by Western blot and RT-qPCR analyses for cyt2Cal and positive citrus identified. Citrus trees expressing different levels of cyt2Cal transcripts were identi-fied (Groups A-C). High expression of the toxin in the leaves (109 transcripts/ng RNA), however, retarded plant growth. The transgenic plants were grown in pots and the roots exposed to 3 week old D. abbreviatus larvae using no-choice plant bioassays. Three cyt2Cal transgenic plants were identified that sustained less root damage be-longing to Group B and C. One plant caused death to 43% of the larvae that fed on its roots expressed 8 x 10(6) cyt2Cal transcripts/ng RNA These results show, for the first time, that Cyt2Cal expressed in moderate amounts by the roots of citrus does not retard citrus growth and can protect it from larval D. abbreviatus. Published by Elsevier Inc.
关键词：Diaprepes abbreviatus Transgenic citrus rootstock cyt2Ca1 Larval control Root re
文献注录：Ben Mahmoud, Sulley Ramos, John E. Shatters, Robert G., Jr. Hall, David G. Lapointe, Stephen L. Niedz, Randall P. Rouge, Pierre Cave, Ronald D. Borovs. Expression of Bacillus thuringiensis cytolytic toxin (Cyt2Ca1) in citrus roots to control Diaprepes abbreviatus larvae [J]. Pesticide Biochemistry and Physiology. 2016, 136: 1-11.
报/刊名：《Pesticide Biochemistry and Physiology》，发表于2016 / 第 136 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 1-11 页 / 共11 页