作者：Feng, Meng-Qi, Lu, Meng-Di, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Wang, Peng-Bo, Liu, Yue, Guo, Wen-Wu and Wu, Xiao-Meng
摘要：Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that transcription factors CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (CsSPL) genes, and reduction of SE efficiency. In the short tandem target mimic (STTM)-miR156a overexpression callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated. csi-miR172d was down-regulated, whereas the target genes, CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus altering starch accumulation in callus cells and regulating SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs-mediated regulation of SE, and provides new insights into enhancing SE capability in citrus. miR156-SPLsand miR172-TOEsaffect starch content and thus alter somatic embryo formation capacity in citrus callus.
文献注录：Feng, Meng-Qi, Lu, Meng-Di, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Wang, Peng-Bo, Liu, Yue, Guo, Wen-Wu and Wu, Xiao-Meng. miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus [J]. . 2022.
报/刊名：《》，发表于2022
文献类型： [J]
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