文献信息：于洁等.  请登录

简介：柑橘是世界上最重要的水果之一。近30年来,世界鲜食柑橘品种的改良主要围绕品质和成熟期等育种目标进行,而果实无核、易剥皮、风味浓和有香味又是决定品质优良的标准。运用传统的育种手段进行柑橘果实品质的改良,具有周期长、受珠心胚干扰、自交不亲和及基因间的紧密连锁等因素的影响,因而进程缓慢。植物基因工程的出现,成为实现目标性状改良的一种快速、有效的途径。通过基因工程诱导单性结实或特异表达种子切除的细胞毒素基因(Barn...

文献信息：Duan YX, Fan J, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：‘Meiwa’ kumquat (Fortunella crassifolia Swingle.) is famous for its relatively short juvenility, delicious flavor, human health benefits and high resistance to citrus canker. To establish kumquat transformation system and to further shorten its juvenility, Agrobacterium-mediated epicotyledon segment transformation of APETALA1 (AP1 from Arabidopsis) gene was conducted. Transformation efficiency r...

文献信息：Khan EU, Fu XZ, Wang J, Fan QJ, Huang XS, Zhang GN, Shi J, Liu JH.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

文献信息：Zeng LH, Xu HF, Zeng YQ, Luan AY, Wang HQ.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT】 | 请登录

文献信息：Reyes CA, Pena EJ, Zanek MC, Sanchez DV, Grau O, Garcia ML.等.  发表于【Plant Cell Reports】 | 请登录

简介：Citrus psorosis virus (CPsV), genus Ophiovirus, family Ophioviridae, is the causal agent of a serious disease affecting citrus trees in many countries. The viral genome consists of three ssRNAs of negative polarity. Post-transcriptional gene silencing (PTGS), a mechanism of plant defence against viruses, can be induced by transgenic expression of virus-derived sequences encoding hairpin RNAs. Sinc...

文献信息：Dutt M, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus sinensis Osb. 9 Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus sinensis (L.) Osbeck) and Mexican Lime (Citrus aurantifolia Swingle) cultivars using a vector containing a bifunctional egfp-nptII fusion gene is described. Several parameters were investigated to optimize genetic trans...

文献信息：Barbosa-Mendes JM, Mourão Filho FAA, Bergamin Filho A, Harakava R, Beer SV, Mendes BMJ.等.  发表于【Scientia Horticulturae】 | 请登录

简介：Transgenic Citrus sinensis (L.) Osb. cv. Hamlin plants expressing the hrpN gene were obtained by Agrobacterium tumefaciens (Smith and Towns) Conn-mediated transformation. hrpN encodes a harpin protein, which elicits the hypersensitive response and systemic acquired resistance in plants. The gene construct consisted of gst1, a pathogen-inducible promoter, a signal peptide for protein secretion to t...

文献信息：Pasquali G, Orbović V, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Transgenic grapefruit plants (Citrus paradisi cv. ‘Duncan’) with the isopentenyltransferase (ipt) gene under the control of APETALA3 promoter have been produced using Agrobacterium-mediated transformation. The relative expression level of the ipt gene was between 2.3 and 7 times higher in transformed plants than in the wild-type but despite the presence of a tissue-specific promoter, the express...

文献信息：Tavano ECR, Stipp LCL, Muniz FR, Mourao FAA, Mendes BMJ.等.  发表于【BIOLOGIA PLANTARUM】 | 请登录

文献信息：杨莉, 张家银, 谢玉明, 杨迎花, 李琳洁, 覃磊, 邓子牛.等.  发表于【经济林研究】 |  , 请登录

简介：Citrus genetic transformation using mature tissue as explant is important for the precocious evaluation of the genetically modified characteristics, which is especially indispensable for seedless cultivars like‘Bingtang’orange. In this study, a protocol was improved for regeneration and genetic transformation with mature tissues of‘Bingtang’orange. The internodal stem segments were sampled fro...

文献信息：Kim JH, Jin SB, Park JW, Boo KH, Bachchu MA, Yun JH, Lee HY, Riu KZ.等.  发表于【Plant Biology (Rockville)】 | 请登录

文献信息：Aleza P, Juárez J, Hernández M, Pina JA, Ollitrault P, Navarro L.等.  发表于【BMC Plant Biology】 |  , 请登录

简介：Background: In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic...

文献信息：Nishikawa F, Endo T, Shimada T, Fujii H, Shimizu T, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

简介：To obtain a peel-specific promoter useful for citrus transgenic research, the 5' flanking region of a citrus dlimonene synthase gene (CitMTSE2), which showed a high degree of expression in the peel of mature fruits, was isolated from the satsuma mandarin (Citrus unshiu Marc.) Bacterial Artificial Chromosome (BAC) library. Promoter activity was characterized by particle bombardment, agroinjection a...

文献信息：Deng W, Luo KM, Li ZG, Yang YW, Hu N, Wu Y.等.  发表于【PLANTA】 | 请登录

文献信息：彭爱红, 彭祝春, 何永睿, 许兰珍, 雷天刚, 刘小丰, 姚利晓, 陈善春.等.  发表于【分子植物育种】 |  , 请登录

简介：With the mature axillary buds of citrus excellent variety Shinanui(Citrus Reticulate×Citrus sinensis) severed as the explant,The plantlets which carried the hepatitis a virus capsid protein fusion gene were obtained through Agrobacterium-mediated transformation.In this study,survival in graft of mature axillary buds of shinanui was the highest,up to 90%,when the orange rootstocks were cultured 15...

文献信息：Dutt M, Grosser JW.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Tong Z, Tan B, Zhang JC, Hu ZY, Guo WW, Deng XX.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

文献信息：Zhang J, Tao N, Xu Q, Zhou W, Cao H, Xu J, Deng X.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：Citrus, rich in carotenoids, is the most important fruit crop based on the total annual production. In the carotenoid biosynthesis pathway, phytoene synthase (PSY, EC 2.5.1.32) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (GGPP) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. In this study, we investigated catalytic activ...

文献信息：Mendes AFD, Cidade L, de Oliveira MLP, Otoni WC, Soares WD, Costa M.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sine...

文献信息：段艳欣, 范净, 郭文武.等.  发表于【中国农学通报】 |  , 请登录

简介：‘Nanfeng’tangerine (Citrus reticulata), favorite for its sweet-fleshed and thin-rind fruit, is one of the oldest citrus cultivars in China. However, the decline of its fruit quality affected its commodity value. In order to explore a rapid and effective breeding programme for cultivar improvement, we performed Agrobacterium-mediated transformation of this cultivar using embryogenic callus induce...