作者:胡春华, 谢玉明, 黄训才, 郭琛, 焦徕, 吴芯茹, 邓子牛.
摘要:In vitro propagation makes it possible to speed the multiplication of the rol A,B,C transgenic citrange plants,SO it is possible to quickly evaluate this new rootstock.Using bud sticks of the transgenic clones(B,D,E)and the control citrange as explants,culture media containing various plant growth regulators were tested for bud bumt,shoot multiplication and rooting.The results indicated that MS medium containing 1 mg/L 6-BA resulted in fastest bud bumting,MS added with 6-BA 0.5 mg/L+ NAA 0.05 mg/L was the best choice for shoot multiplication.and 1/2MS supplied with NAA 0.5 mg/L and 0.2% activated charcoal showed quite suitable for rooting. Fhe transgenic clones had significantly higher rooting percentage (93.3%)than that of the control(66.7%).
关键词:rol A,B,C genes;Transgenic citrange;Tissue culture;Micropropagation
Key words: rolABC基因;转基因枳橙:组织培养;快速繁殖
文献注录:胡春华, 谢玉明, 黄训才, 郭琛, 焦徕, 吴芯茹, 邓子牛.. 转rolA、B、C基因枳橙快繁技术 [J]. 果树学报. 2006, 01: 142-144.
报/刊名:《果树学报》,发表于2006 / 第 01 期。
文献类型: [J]
页码:第 142-144 页 / 共3 页