作者：Peng Aihong，Xu Lanzhen，He Yongrui，Lei Tiangang，Yao Lixiao，Chen Shanchun,Zou, Xiuping
作者单位：National Center for Citrus Variety Improvement, Citrus，Research Institute, Southwest University, Chongqing 400712, People’s Republic of China
摘要：Marker-free transgenic plants alleviate concerns regarding the biosafety of genetically modified organisms and promote their commercialization. In this study, a transformation vector pLI35SAAT, harboring a Cre/loxP-mediated recombination system combined with the isopentenyl transferase (ipt) selectable marker gene and an anti-bacterial peptide gene AATCB, was used to produce marker-free transgenic \'Tarocco\' blood orange (Citrus sinensis Osbeck) with enhanced resistance to citrus canker. Using ipt positive selection, a transformation efficiency of 21.4 % was achieved. When the DNA between two loxP sites was excised, phenotypically normal shoots gradually appeared from 74.8 % of the transgenic ipt shoots. Their marker-free transgenic nature was confirmed using PCR and sequencing analyses. In vitro evaluations of citrus canker disease resistance revealed that marker-free transgenic plants exhibited an enhanced resistance to Xanthomonas axonopodis pv. citri. The marker-free transgenic plants appeared phenotypically normal under greenhouse conditions. Thus, marker-free transgenic citrus plants with targeted traits can be efficiently produced using a Cre/loxP-mediated recombination system combined with ipt positive selection.
关键词：Cre/loxP recombination system , Ipt positive,selection , Marker-free transgenic
文献注录：Peng Aihong，Xu Lanzhen，He Yongrui，Lei Tiangang，Yao Lixiao，Chen Shanchun,Zou, Xiuping. Efficient production of marker-free transgenic \'Tarocco\' blood orange (Citrus sinensis Osbeck) with enhanced resistance to citrus canker using a Cre/loxP site-recombination system [J]. Plant Cell Tissue and Organ Culture. 2015, 123(1): 1-13.
报/刊名：《Plant Cell Tissue and Organ Culture》，发表于2015 / 第 123(1) 期。
文献类型： [J]
页码：第 1-13 页 / 共13 页