作者：Peng, Aihong Xu, Lanzhen He, Yongrui Lei, Tiangang Yao, Lixiao Chen, Shanchun Zou, Xiuping
作者单位：National Center for Citrus Variety Improvement, Citrus Research Institute, Southwest University, Chongqing 400712, People’s Republic of China
摘要：Marker-free transgenic plants alleviate con-
cerns regarding the biosafety of genetically modified
organisms and promote their commercialization. In this
study, a transformation vector pLI35SAAT, harboring a
Cre/loxP-mediated recombination system combined with
the isopentenyl transferase (ipt) selectable marker gene and
an anti-bacterial peptide gene AATCB, was used to produce
marker-free transgenic ‘Tarocco’ blood orange (Citrus
sinensis Osbeck) with enhanced resistance to citrus canker.
Using ipt positive selection, a transformation efficiency of
21.4 % was achieved. When the DNA between two loxP
sites was excised, phenotypically normal shoots gradually
appeared from 74.8 % of the transgenic ipt shoots. Their
marker-free transgenic nature was confirmed using PCR
and sequencing analyses. In vitro evaluations of citrus
canker disease resistance revealed that marker-free trans-
genic plants exhibited an enhanced resistance to Xan-
thomonas axonopodis pv. citri. The marker-free transgenic
plants appeared phenotypically normal under greenhouse
conditions. Thus, marker-free transgenic citrus plants with
targeted traits can be efficiently produced using a Cre/loxP-
mediated recombination system combined with ipt positive
selection.
关键词：Cre/loxP recombination system ? Ipt positive selection ? Marker-free transgenic
文献注录：Peng, Aihong Xu, Lanzhen He, Yongrui Lei, Tiangang Yao, Lixiao Chen, Shanchun Zou, Xiuping. Efficient production of marker-free transgenic ‘Tarocco’ blood orange (Citrus sinensis Osbeck) with enhanced resistance to citrus canker using a Cre/loxP site-recombination system [J]. Plant Cell, Tissue and Organ Culture (PCTOC). 2015, 123: 1-13.
报/刊名：《Plant Cell, Tissue and Organ Culture (PCTOC)》，发表于2015 / 第 123 期。
文献类型： [J]
页码：第 1-13 页 / 共13 页