文献信息：杨莉等.  请登录

简介：转基因育种是常规育种的重要辅助手段，但人们担心标记基因的食品安全问题，因此，建立柑橘无选择标记的转化体系，对提高转基因作物的安全性意义重大。柑橘溃疡病是一种世界性的检疫性病害，对产业危害极大，因缺乏抗病资源和有效防控方法，该病仍无法根除。通过转基因育种，抑制病原菌致病因子的功能，获得抗病种质，是一种创新，为彻底控制溃疡病打下基础。本研究的结果如下： 1.通过将pX6-gfp中的启动子G10-90替换成CaMV35S，构...

文献信息：张倩等.  请登录

简介：柑橘是世界第一大水果，通过遗传改良新技术创造优良柑橘品种是提升我国柑橘市场竞争力的重要措施。珠心胚干扰、性器官败育、童期长、遗传上高度杂合等生物学特征严重阻碍了柑橘常规育种的进程。近年来，不断完善的转基因技术为柑橘品种改良提供了一种更加快速有效的途径。 本研究以柑橘原生质体为转化受体，以绿色荧光蛋白基因(Green fluorescentprotein gene，GFP)作为报告基因，建立了原生质体电击转化体系，同时对影响电击转化...

文献信息：卫国琴等.  请登录

简介：柑橘经常受到冻害、干旱、盐碱等环境因素的胁迫，严重阻碍了柑橘业的健康发展。因此，研究植物对环境胁迫的应答机制，利用基因工程手段提高植物的抗逆性，已成为柑橘业发展的关键性工作。 CBF(C-repeat binding factor)是调控一系列植物冷驯化相关基因表达的转录因子，被低温诱导表达，与相应顺式作用元件CRT/DRE(C-repeat/dehydrationresponsive element)结合，诱导冷相关基因的表达，进而合成抗寒相关的糖类、脯氨酸和膜稳定蛋白...

文献信息：敖小平等.  请登录

简介：柑桔常规育种开始于19世纪，但是，由于柑桔存在童期长、杂合性高、种子多胚、遗传上不亲和等现象，常规育种受到限制。生物技术(尤其是组织培养及基因工程技术)帮助育种者有效地克服了传统育种中的各种障碍。同时，遗传转化技术因其不受基因型限制，可以保持品种的完整性，同时可以定向地增加或者删除植物的某个特性，已成为提高柑桔及其他物质品质的非常有吸引力的方法。然而，要获得高效的遗传转化系统，必须建立高效的再生体系。...

文献信息：王会全等.  请登录

简介：柑桔(Citrus L.)是当今世界最重要的水果之一,也是我国南方最重要的经济果树。柑桔是多年生木本果树,以往的常规育种由于存在童期长、遗传高度杂合性和多胚现象,使得柑桔育种工作困难重重。近年来,不断完善的转基因技术为我们提供了新的育种和品种改良手段。 目前,转基因作物的安全性问题受到极大关注。利用遗传转化技术改良柑桔品种首先要考虑生物安全性问题,使用安全的选择标记基因是有效方法之一。 本实验以福建传统优良甜橙品种...

文献信息：于洁等.  请登录

简介：柑橘是世界上最重要的水果之一。近30年来,世界鲜食柑橘品种的改良主要围绕品质和成熟期等育种目标进行,而果实无核、易剥皮、风味浓和有香味又是决定品质优良的标准。运用传统的育种手段进行柑橘果实品质的改良,具有周期长、受珠心胚干扰、自交不亲和及基因间的紧密连锁等因素的影响,因而进程缓慢。植物基因工程的出现,成为实现目标性状改良的一种快速、有效的途径。通过基因工程诱导单性结实或特异表达种子切除的细胞毒素基因(Barn...

文献信息：Duan YX, Fan J, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：‘Meiwa’ kumquat (Fortunella crassifolia Swingle.) is famous for its relatively short juvenility, delicious flavor, human health benefits and high resistance to citrus canker. To establish kumquat transformation system and to further shorten its juvenility, Agrobacterium-mediated epicotyledon segment transformation of APETALA1 (AP1 from Arabidopsis) gene was conducted. Transformation efficiency r...

文献信息：Khan EU, Fu XZ, Wang J, Fan QJ, Huang XS, Zhang GN, Shi J, Liu JH.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

文献信息：Zeng LH, Xu HF, Zeng YQ, Luan AY, Wang HQ.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT】 | 请登录

文献信息：Reyes CA, Pena EJ, Zanek MC, Sanchez DV, Grau O, Garcia ML.等.  发表于【Plant Cell Reports】 | 请登录

简介：Citrus psorosis virus (CPsV), genus Ophiovirus, family Ophioviridae, is the causal agent of a serious disease affecting citrus trees in many countries. The viral genome consists of three ssRNAs of negative polarity. Post-transcriptional gene silencing (PTGS), a mechanism of plant defence against viruses, can be induced by transgenic expression of virus-derived sequences encoding hairpin RNAs. Sinc...

文献信息：Dutt M, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus sinensis Osb. 9 Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus sinensis (L.) Osbeck) and Mexican Lime (Citrus aurantifolia Swingle) cultivars using a vector containing a bifunctional egfp-nptII fusion gene is described. Several parameters were investigated to optimize genetic trans...

文献信息：Barbosa-Mendes JM, Mourão Filho FAA, Bergamin Filho A, Harakava R, Beer SV, Mendes BMJ.等.  发表于【Scientia Horticulturae】 | 请登录

简介：Transgenic Citrus sinensis (L.) Osb. cv. Hamlin plants expressing the hrpN gene were obtained by Agrobacterium tumefaciens (Smith and Towns) Conn-mediated transformation. hrpN encodes a harpin protein, which elicits the hypersensitive response and systemic acquired resistance in plants. The gene construct consisted of gst1, a pathogen-inducible promoter, a signal peptide for protein secretion to t...

文献信息：Pasquali G, Orbović V, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Transgenic grapefruit plants (Citrus paradisi cv. ‘Duncan’) with the isopentenyltransferase (ipt) gene under the control of APETALA3 promoter have been produced using Agrobacterium-mediated transformation. The relative expression level of the ipt gene was between 2.3 and 7 times higher in transformed plants than in the wild-type but despite the presence of a tissue-specific promoter, the express...

文献信息：Tavano ECR, Stipp LCL, Muniz FR, Mourao FAA, Mendes BMJ.等.  发表于【BIOLOGIA PLANTARUM】 | 请登录

文献信息：杨莉, 张家银, 谢玉明, 杨迎花, 李琳洁, 覃磊, 邓子牛.等.  发表于【经济林研究】 |  , 请登录

简介：Citrus genetic transformation using mature tissue as explant is important for the precocious evaluation of the genetically modified characteristics, which is especially indispensable for seedless cultivars like‘Bingtang’orange. In this study, a protocol was improved for regeneration and genetic transformation with mature tissues of‘Bingtang’orange. The internodal stem segments were sampled fro...

文献信息：Kim JH, Jin SB, Park JW, Boo KH, Bachchu MA, Yun JH, Lee HY, Riu KZ.等.  发表于【Plant Biology (Rockville)】 | 请登录

文献信息：Aleza P, Juárez J, Hernández M, Pina JA, Ollitrault P, Navarro L.等.  发表于【BMC Plant Biology】 |  , 请登录

简介：Background: In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic...

文献信息：Nishikawa F, Endo T, Shimada T, Fujii H, Shimizu T, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

简介：To obtain a peel-specific promoter useful for citrus transgenic research, the 5' flanking region of a citrus dlimonene synthase gene (CitMTSE2), which showed a high degree of expression in the peel of mature fruits, was isolated from the satsuma mandarin (Citrus unshiu Marc.) Bacterial Artificial Chromosome (BAC) library. Promoter activity was characterized by particle bombardment, agroinjection a...

文献信息：Deng W, Luo KM, Li ZG, Yang YW, Hu N, Wu Y.等.  发表于【PLANTA】 | 请登录

文献信息：彭爱红, 彭祝春, 何永睿, 许兰珍, 雷天刚, 刘小丰, 姚利晓, 陈善春.等.  发表于【分子植物育种】 |  , 请登录

简介：With the mature axillary buds of citrus excellent variety Shinanui(Citrus Reticulate×Citrus sinensis) severed as the explant,The plantlets which carried the hepatitis a virus capsid protein fusion gene were obtained through Agrobacterium-mediated transformation.In this study,survival in graft of mature axillary buds of shinanui was the highest,up to 90%,when the orange rootstocks were cultured 15...