文献信息：da Silva RP, Costa MAPD, Souza AD, de Almeida WAB.等.  发表于【PESQUISA AGROPECUARIA BRASILEIRA】 |  , 请登录

文献信息：谢玉明, 邓子牛, 郭琛, 焦徕, 胡春华, 熊兴耀.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：Seedling epicotyl (intemodal stem) segments of‘Xupu’sweet orange(Citrus sinensis L.0sb.) were used as expIants to establish Agrobacterium—mediated transfomation system with chit42 and phyB genes. The results showed that trallsformed buds could be obtained after the epicotyl segments were cultured on co-culture medium(MS+3 mg／L BA) for 3 days and then on selective medium with 100 mg／L Kanamyc...

文献信息：杨晓红, 孙中海, 仝瑞建.等.  发表于【中国农业科学】 | 请登录

简介：Genetic transformation experiments of the different exploids from Citrus grandis cv.Changshou Shatian You infected with Agrobacterium rhizogenes were carried out under dark and light.The optimized culture system for Ri T-DNA transformed roots of C.grandis cv.Changshou Shatian You was constructed as follows:After the ventral wounded striations on the single activation cotyledon were inoculated by A...

文献信息：Guo W, Duan Y, Olivares-Fuster O, Wu Z, Arias CR, Burns JK, Grosser JW.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：Valencia orange [Citrus sinensis (L.) Osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (TSPME) reduces its juice quality. A long-term strategy of this work is to eliminate or greatly reduce TSPME activity in Valencia orange. Previous work resulted in the isolation of a putative TSPME gene, CsPME4...

文献信息：Rosely Pereira da Silva, Maria Angélica Pereira de Carvalho Costa, Antonio da Silva Souza, Weliton Antonio Bastos de Almeida.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this work was to establish an efficient protocol for in vitro regeneration of plants through organogenesis of sweet orange ‘Pera’ (Citrus sinensis L. Osbeck) juvenile explants, in order to give support for future studies of genetic transformation. Epicotyl segments used as explants were introduced in MT culture media. To maximize the regeneration of in vitro plants, experiments ...

文献信息：周春丽, 郭卫东, 王德解, 余初浪, 路梅, 李玉萍.等.  发表于【西北植物学报】 |  , 请登录

简介：Bergamot is a plant of high medicinal and ornamental values that belongs toCitrus medicaL.var. sarcodactylis(Noot.) Swingle,but does not have many varieties.Thus it is necessary to conduct its genetic improvement and variety breeding.In order to apply particle bombardment to the genetic improvement and variety breeding of bergamot,this study,usingGUSgene as the report gene,transformedGUSgene into ...

文献信息：李东栋, 邓秀新.等.  发表于【分子植物育种】 |  , 请登录

简介：In this study,15 kinds of calluses from different citrus varieties were transformed following the developed system.The result showed that12 kinds of varieties'calluses among 15 could receive a certain number of resistant calluses, and 3 varieties' could not obtain any resistant calluses.Transgenic plants of 4 kinds of cultivars were first reported in this papersuch as Guoqing No.1,Hong Kong Kum qu...

文献信息：Cirvilleri, Spina S, Scuderi G, Gentile A, Catara A.等.  发表于【Journal of Plant Pathology】 | 请登录

简介：To study the influence of rolABC Troyer citrange plants on potentially beneficial root-associated bacteria, transgenic and non-transgenic plant lines were monitored over the course of one year. Culturable bacterial populations were not significantly different between rolABC and wild type lines, whereas the population of fluorescent pseudomonads varied according to the plant lines and the date of s...

文献信息：郭卫东, 周春丽, 路梅, 谢选松.等.  发表于【浙江师范大学学报(自然科学版)】 |  , 请登录

简介：Tender and elder leaf-discs of fingered-citrus (Citrus medicaL. var.sarcodactylis(Noot) Swingle) were transformed withGusgene via biolistics method. The transient expression of Gus genewas used to analyse the transformation rate under different target distance. The results indicated that a higher transformation rate according to transient expression ofGusgene could be acquired from tender leaf-dis...

文献信息：Omar AA, Grosser JW等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Huang JQ, Sun ZH.等.  发表于【Agricultural Sciences in China】 |  , 请登录

简介：The Arabidopsis ICE1(inducer of CBF expression 1)gene was cloned through RT-PCR of Arabidopsis cDNAs and introduced into the lemon(Citrus Limon(L.)Burm.F.cv.Eureka)genome using Agrobacterium-mediated transformation method.Epicotyl segments from in vitro grown lemon seedlings were co-cultivated with A.tumefaciens strain EHA 105 carrying the binary plasmid pMVICE1,whose T-DNA region contain ICE1 gen...

文献信息：Vieira JGZ, Faria RT, Vieira LGE, Molinari HBC.等.  发表于【Acta Scientiarum Agronomy】 | 请登录

文献信息：Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Yasushi Kobayashi, Takashi Araki, Mitsuo Omura.等.  发表于【Transgenic Research】 |  , 请登录

简介：Citrus FT (CiFT) cDNA, which promoted the transition from the vegetative to the reproductive phase in Arabidopsis thaliana, when constitutively expressed was introduced into trifoliate orange (Poncirus trifoliata L. Raf.). The transgenic plants in which CiFT was expressed constitutively showed early flowering, fruiting, and characteristic morphological changes. They started to flower as early as 1...

文献信息：胡桂兵, 张上隆, 徐昌杰, 林顺权.等.  发表于【果树学报】 |  , 请登录

简介：Based on the codon usage for the amino acids of 177 citrus functional proteins deposited in GenBank, a new antibacterial peptide D (APD) gene with citrus preference codons was designed and chemcially synthesized. The peptide has anability to kill the phloem conducted pathogens for Huanglonbing, a destructive and widespread disease for citrus. The new gene encoding the same peptide, referred to as ...

文献信息：Han SH, Ahn HJ, Kang SK, Kim HY.等.  发表于【Journal of the Korean Society for Horticultural Science】 | 请登录

文献信息：敖小平, 胡新喜, 郭琛, 焦徕, 邓子牛, 熊兴耀.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：Internodal stem segments from 30 d old seedlings of‘Dahong’sweet orange(Citrus sinensis Osbeck)were cultured on bud induction media，to study on the genetic transformation in Citrus with rolB gene．The best medium for induction was MS+3.0 mg/L 6-BA，which led to 95%of explants producing at least one bud，so this medium could be considered as the best for bud induction in plant regeneration．The ...

文献信息：Gonzalez-Ramos J, Graham J, Mirkov T.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息： Liu, Ji Hong等.  发表于【 Journal of Agricultural Biotechnology】 | 请登录

简介： Protoplast fusion can circumvent reproductive barriers facing citrus, such as polyembryony and female and/or male sterility, and paves way for citrus cultivar improvement. Two kinds of fusion patterns were employed for citrus, leaf-derived protoplasts + embryogenic callus protoplasts and embryogenic callus protoplasts + embryogenic callus protoplasts. More than 200 cases of somatic hybrids have b...

文献信息：HU Rong，H Wei，SC Chen，HE Yong-Rui等.  发表于【Hereditas Beijing】 | 请登录

简介：The use of edible for the production and delivery of vaccine could provide an economical alternative to systems. The construction of the plant expression vector pBI121-A was reported, which contained a fusion gene encoding A . The gene was located between the left and right Ti border sequences under the control of CaMV35S promoter. The vector was identified via PCR and restriction enzyme analysis ...

文献信息：Xu, X. Y.,Liu, J. H.,Deng, X. X.等.  发表于【Euphytica】 |  , 请登录

简介：Oleander is a Mediterranean evergreen shrub found along watercourses, gravelly places and damp slopes. It is
<br />grown widely as an ornamental for its abundant and long-lasting flowering as well as its moderate hardiness.
<br />Genetic relatedness among 71 accessions, including commercial varieties, different sources of the same varieties,
<br />and selections from the wild were investigated ...