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  • 981. Comparison Between Mannose and Kanamycin Selection Systems for Genetic Transformation of Citrus

    文献信息:Dutt M, Grosser JW.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

    982. Using precocious trifoliate orange (Poncirus trifoliata [L.] Raf.) to establish a short juvenile transformation platform for citrus

    文献信息:Tong Z, Tan B, Zhang JC, Hu ZY, Guo WW, Deng XX.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

    983. Functional characterization of Citrus PSY gene in Hongkong kumquat (Fortunella hindsii Swingle)

    文献信息:Zhang J, Tao N, Xu Q, Zhou W, Cao H, Xu J, Deng X.等.  发表于【Plant Cell Reports】 |  , 请登录

    简介:Citrus, rich in carotenoids, is the most important fruit crop based on the total annual production. In the carotenoid biosynthesis pathway, phytoene synthase (PSY, EC 2.5.1.32) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (GGPP) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. In this study, we investigated catalytic activ...

    984. Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.

    文献信息:Mendes AFD, Cidade L, de Oliveira MLP, Otoni WC, Soares WD, Costa M.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

    简介:Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sine...

    985. 南丰蜜橘胚性愈伤组织诱导及其转化研究

    文献信息:段艳欣, 范净, 郭文武.等.  发表于【中国农学通报】 |  , 请登录

    简介:‘Nanfeng’tangerine (Citrus reticulata), favorite for its sweet-fleshed and thin-rind fruit, is one of the oldest citrus cultivars in China. However, the decline of its fruit quality affected its commodity value. In order to explore a rapid and effective breeding programme for cultivar improvement, we performed Agrobacterium-mediated transformation of this cultivar using embryogenic callus induce...

    986. The Effect of Agrobacterium Growth Phase and Density on Citrus Transformation

    文献信息:Niedz RP, Evens TJ.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

    987. Highly efficient transformation of the GFP and MAC12.2 genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus

    文献信息:Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW.等.  发表于【Tree Genetics & Genomes】 | 请登录

    简介:Precocious trifoliate orange (Poncirus trifoliata [L.] Raf), an extremely early flowering mutant of P. trifoliata, is an attractive model for functional genomics research in Citrus. A procedure for efficient regeneration and transformation of this genotype was developed by using green fluorescent protein (GFP) gene as visual marker and etiolated stem segments as explants. In vivo monitoring of GFP...

    988. Resistance to bacterial spot can be correlated with resistance to canker in transgenic citrus

    文献信息:Dutt M, Barthe G, Francis M, Graham J, Grosser J.等.  发表于【PHYTOPATHOLOGY】 | 请登录

    989. 早实枳模式转化验证体系建立及基因工程创造柑橘无核新种质(摘要)

    文献信息:谭彬, 郭文武.等.  发表于【《第二届全国果树分子生物学学术研讨会论文集》】 | 请登录

    990. Early Flowering Phenotype in Transgenic Pears (Pyrus communis L.) Expressing the CiFT Gene

    文献信息:Matsuda N, Ikeda K, Kurosaka M, Takashina T, Isuzugawa K, Endo T, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

    简介:We transformed the European pear (Pyrus communis L.) ‘La France’ and ‘Ballade’ with the Citrus FLOWERING LOCUS T (CiFT) gene, which induces early flowering. Subsequent DNA blot analysis of the transformed and wild-type plants indicated that the transformed plants carried 1–4 copies of CiFT. Of the 8 transformed ‘La France’ and 7 transgenic ‘Ballade’ plant lines obtained, 7 and 5 lines res...

    991. Decreasing unpalatable flavonoid components inCitrus: the effect of transformation construct

    文献信息:Koca U, Berhow MA, Febres VJ, Champ KI, Carrillo-Mendoza O, Moore GA.等.  发表于【Physiologia Plantarum】 | 请登录

    简介:Citrus species accumulate large quantities of flavanone glycosides in their leaves and fruit. The physiological role(s) of these compounds in citrus plants are unknown, but they have been documented to benefit human health upon consumption. Flavanone rutinosides are tasteless, whereas flavanone neohesperidosides, such as naringin, give a bitter taste to fruit and fruit juice products, reducing the...

    992. Gene stacking in 1-year-cycling APETALA1 citrus plants for a rapid evaluation of transgenic traits in reproductive tissues

    文献信息:Cervera M, Navarro L, Peña L等.  发表于【Journal of Biotechnology】 |  , 请登录

    简介:Rapid flowering is crucial to perform functional genomic studies to investigate reproductive biology characteristics and fruit quality-related traits in fruit trees. However, long generation cycles of woody plants considerably delay this evaluation. Through genetic transformation, juvenile periods can be significantly shortened by overexpression of flower meristem-identity genes. Transgenic APETAL...

    993. Development of Citrus Cultivars Resistant to Huanglongbing Disease by Transformation with AMP Genes

    文献信息:Bowman KD, McCollum G, Niedz R, Stover E, Albrecht U, Stange R.等.  发表于【HORTSCIENCE】 | 请登录

    994. Morphogenesis and plantlet regeneration from hypocotyl explants in three selected species of citrus

    文献信息:Vibhute M, Tiwari R, Tripathi MK, Baghel BS, Tiwari S.等.  发表于【Plant Cell Biotechnology and Molecular Biology】 | 请登录

    995. Cultivar-dependent Gene Transfer into Citrus using Agrobacterium

    文献信息:Dutt, Manjul Orbovi, Vladimir Grosser, Jude W.等.  发表于【Proceedings of the ... annual meeting of the Florida State H】 | 请登录

    996. Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit

    文献信息:Endo T, Shimada T, Fujii H, Nishikawa F, Sugiyama A, Nakano M, Shimizu T, Kobayashi Y, Araki T, Peña L, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

    简介:We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate ora...

    997. 苹果亚精胺合成酶基因(MdSPDS1)转化甜橙及转基因植株溃疡病抗性研究(摘要)

    文献信息:付行政, 陈传武, 王引, 刘继红等.  发表于【《第二届全国果树分子生物学学术研讨会论文集》】 |  , 请登录

    998. Regeneration of transgenic citrus plants from the trimmed shoot-root region of etiolated seedlings.

    文献信息:LI D. L., TAN B., DUAN Y. X., GUO W. W.等.  发表于【Biologia Plantarum】 |  , 请登录

    简介:Transformation and high efficient regeneration of transgenic plants from the trimmed etiolated shoot/root region (TESRR) of Anliucheng sweet orange [Citrus sinensis (L.) Osb.] seedling was reported. A visual green fluorescent protein (GFP) marker gene was introduced to evaluate transformation efficiency by using the explants from TESRR and epicotyls. The transformation protocol was: infection 20 m...

    999. Assessment of polyembryony in lemon: rescue and in vitro culture of immature embryos

    文献信息:Perez-Tornero, O.,Porras, I.等.  发表于【Plant Cell, Tissue and Organ Culture】 |  , 请登录

    简介:The 27 lemon cultivars analysed could be considered slightly or moderately polyembryonic, with 25 to 43% of seeds being polyembryonic and from 1.3 to 1.6 embryos per seed. On this basis, it is necessary to rescue zygotic embryos at an immature stage. Rescue and in vitro embryo development have been studied in two Citrus limon polyembryonic cultivars. Sucrose (50 and 70 g/l) was combined with Muras...

    1000. Cytogenetic analysis of two interspecific Citrus allotetraploid somatic hybrids and their diploid fusion parents

    文献信息:Chen, C. L.,Guo, W. W.,Yi, H. L.,Deng, X. X.等.  发表于【Plant Breeding】 |  , 请登录

    简介:Microsporogenesis of two interspecific allotetraploid somatic hybrids, i.e. 'Hamlin' sweet orange + 'Rough Lemon' (HR) and 'Key' lime + 'Valencia' sweet orange (KV), was examined and compared with their corresponding diploid fusion parents. Meiotic analysis revealed that only 13.2 and 18.0% of the meiocytes exhibited normal tetrad formation in HR and KV somatic hybrid plants, respectively, and irr...

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