文献信息:Costa MGC, Alves VS, Lani ERG, Mosquim PR, Carvalho CR , Otoni WC.等. 发表于【SCIENTIA HORTICULTURAE】 | 请登录
文献信息:Peña L, Pérez RM, Cervera M, Juárez JA, Navarro L.等. 发表于【Annals of Botany】 | , 请登录
简介:Background and Aims: Genetic transformation of plants relies on two independent but concurrent processes: integration of foreign DNA into plant cells and regeneration of whole plants from these transformed cells. Cell competence for regeneration and for transformation does not always fall into the same cell type/developmental stage, and this is one of the main causes of the so-called recalcitrance...
文献信息:Koca U, Moore G, Berhow M, Eyal Y, Frydman A.等. 发表于【Plant Biology (Rockville)】 | 请登录
文献信息:Orbovic V, Ananthakrishnan G, Gowda S, Dawson WO, Grosser JW.等. 发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录
文献信息:Molinari HBC,Marur CJ, Filho JCB, Kobayashi AK, Pileggi M, Júnior RPL, Pereira LFP, Vieira LGE.等. 发表于【Plant Science】 | , 请登录
简介:In the present study, we have analyzed transformed plants of the citrus rootstock Carrizo citrange constitutively expressing a Δ1-pyrroline- 5-carboxylate synthetase mutant gene (p5cs), which encodes the rate-limiting enzyme of the proline biosynthetic pathway. Transgenic plants grew normally in vivo and accumulated high levels of proline in the leaves. In well-watered conditions, the photosynthe...
文献信息:吴耀军, 杜晓莉, 韩美丽, 黄华艳.等. 发表于【广西林业科学】 | , 请登录
文献信息:Gentile A, Deng ZN, Malfa SL, Domina F, Germanà C, Tribulato E.等. 发表于【Acta Horticulturae】 | 请登录
文献信息:Lucker J, Schwab W, van Hautum B, Blaas J, van der Plas LHW, Bouwmeester HJ, Verhoeven HA.等. 发表于【Plant Physiology】 | , 请登录
简介:Wild-type tobacco (Nicotiana tabacum) plants emit low levels of terpenoids, particularly from the flowers. By genetic modification of tobacco cv Petit Havana SR1 using three different monoterpene synthases from lemon (Citrus limon L. Burm. f.) and the subsequent combination of these three into one plant by crossings, we show that it is possible to increase the amount and alter the composition of t...
文献信息:Molinari HBC, Bespalhok JC, Kobayashi AK, Pereira LFP, Vieira LGE.等. 发表于【Scientia Horticulturae】 | , 请登录
简介:Transgenic plants of the important citrus rootstock Swingle citrumelo (Citrus paradisi Macf. × Poncirus trifoliata L. Raf.) were obtained using Agrobacterium tumefaciens-mediated transformation of seedling epicotyl tissue. Thin sections were co-cultured with Agrobacterium strain EHA105 carrying pBI121, a plasmid that contains the nptII (pnos) gene and the uidA (p35S) gene for scorable marker β-g...
文献信息:何永睿, 邹修平, 彭爱红, 徐忠强, 许兰珍, 彭祝春, 陈善春.等. 发表于【热带作物学报】 | , 请登录
简介:The embryoids of Jincheng orange and Xinhui orange ( Citrus sinensis Osbeck) were induced from nucellar embryo, and the mature shoots were obtained from 20-year-old plants of Beibei447 orange and Navel orange . After they were inoculated with agrobacterium strain LBA4404 harboring vector that contained Shiva A and Cecropin B gene, the effects of various media, concentrations of kanamycin, places o...
文献信息:Gentile A, Deng ZN, Malfa SL, Domina F, Germanà C, Tribulato E.等. 发表于【Acta Hort】 | 请登录
文献信息:Rhim SL, Kim G, Lee JH, Kuo CI, Suh SC, Huang LC.等. 发表于【Journal of Plant Biotechnology】 | 请登录
文献信息:Orbovic V, Govindarajulu A, Grosser JW.等. 发表于【Plant Biology (Rockville)】 | 请登录
文献信息:Domínguez A, Cervera M, Pérez RM. Romero J, Fagoaga C, Cubero J, López MM, Juárez JA, Navarro L, Peña L.等. 发表于【Molecular Breeding】 | 请登录
简介:Genetic transformation has been achieved for several citrus genotypes. However, regeneration of escapes at high frequency is a major problem, making the available procedures rather inefficient. Attempts to improve selection by increasing the concentration of kanamycin, used as the selective agent, or substituting it by geneticin have been unsuccessful. Here, we have critically assessed the actual ...
文献信息:韩美丽, 陆荣生, 杜晓莉 and 覃建林等. 发表于【广西农学报】 | , 请登录
简介:Taking Epicotyls of Shaddock as the testing objective, a research on effecting factors of establishment of gene transformation system of agricultural bacillus media was conducted. The result of the research indicated that the selecting concentration of Chanamycin of Epicotyl of Shaddock was a little bit over 70mg,that the input of As/150uM into the common culture medium of Epicotyls and agricultur...
文献信息:胡蓉, 魏泓, 陈善春, 何永睿.等. 发表于【遗传】 | , 请登录
简介:The use of edible plants for the production and delivery of vaccine proteins could provide an economical alternative to fermentation systems. The construction of the plant expression vector pBI121-A was reported, which comtained a fusion gene encoding hepatitis A capsid proteins. The gene was located between the left and right Ti border sequences under the control of CaMV35S promoter. The vector w...
文献信息:Guo WW, Grosser JW.等. 发表于【Acta horticulturae】 | 请登录
文献信息:Kayim M, Ceccardi TL, Berretta MJ, Barthe GA, Derrick KS.等. 发表于【Plant Cell Reports】 | 请登录
简介:The protein p12 accumulates in leaves of trees with citrus blight (CB), a serious decline of unknown cause. The function of p12 is not known, but sequence analysis indicates it may be related to expansins. In studies to determine the function of p12, sense and antisense constructs were used to make transgenic Carrizo citrange using an Agrobacterium-mediated transformation system. Homogeneous b-glu...
文献信息:Omar AA, Grosser JW.等. 发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录
文献信息:Iwanami T, Shimizu T, Ito T, Hirabayashi T等. 发表于【PLANT DISEASE】 | 请登录