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  • 461. Characterization of transgenic Poncirus trifoliata overexpressing the ferric chelate reductase gene CjFRO2 from Citrus junos

    文献信息:Peng, A. H. Liu, X. F. He, Y. R. Xu, L. Z. Lei, T. G. Yao, L. X. Cao, L. Chen, S. C.等.  2015-03-16 发表于【Biologia Plantarum】 |  , 请登录

    简介:Iron deficiency chlorosis occurs frequently in calcareous soils. The transformation of plants with ferric chelate
    <br />reductase genes (FROs) provides a potential strategy to alleviate plant chlorosis under iron deficiency. A CjFRO2 gene
    <br />isolated from Citrus junos Sieb. ex Tanaka was introduced into Poncirus trifoliata (L.) Raf via Agrobacterium-mediated
    <br />transformation. The transgene ...

    462. Efficient production of marker-free transgenic ‘Tarocco’ blood orange (Citrus sinensis Osbeck) with enhanced resistance to citrus canker using a Cre/loxP site-recombination system

    文献信息:Peng, Aihong Xu, Lanzhen He, Yongrui Lei, Tiangang Yao, Lixiao Chen, Shanchun Zou, Xiuping等.  2015-09-03 发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

    简介:Marker-free transgenic plants alleviate con-
    <br />cerns regarding the biosafety of genetically modified
    <br />organisms and promote their commercialization. In this
    <br />study, a transformation vector pLI35SAAT, harboring a
    <br />Cre/loxP-mediated recombination system combined with
    <br />the isopentenyl transferase (ipt) selectable marker gene and
    <br />an anti-bacterial peptide gene AATCB, was ...

    463. Overexpression of Citrus grandis DREB gene in tomato affects fruit size and accumulation of primary metabolites

    文献信息:Nishawy, Elsayed Sun, Xiaohua Ewas, Mohamed Ziaf, Khurram Xu, Rangwei Wang, Dan Amar, Mohamed Zeng, Yunliu Cheng, Yunjiang等.  发表于【Scientia Horticulturae】 | 请登录

    简介:Citrus is a perennial woody crop of global economic importance. However, the regulatory mechanism of fruit ripening in citrus remains poorly understood. To explore the regulatory roles of transcription factors in fruit ripening, CgDREB (dehydration responsive element binding) gene was characterized and was functionally overexpressed in tomato (Solanum lycopersicum, cv. Ailsa Craig), resulting in s...

    464. Application of simple sequence repeat markers for demarcation of Citrus reticulata nucellar and hybrid seedlings

    文献信息:Mondal, B Pramanick, S Saha, R Karmakar, M等.  2015-01-18 发表于【International Journal of Biosciences (IJB)/http://dx.doi.org/10.12692/ijb/6.2.128-133】 |  , 请登录

    简介:The Polyembryony trait is a characteristic feature of the genus Citrus. This trait is regarded as a nuisance in hybridization but could be effectively utilized for Citrus propagation. The occurrence of nucellar embryony could be regarded as a replacement of costly tissue culture technique and identification of nucellar seedlings of Citrus reticulata may lead to proper management of the elite germp...

    465. Abiotic Stress and Hormonal Responses of a Promoter of Citrus Carotenoid Isomerase Gene in Transgenic Arabidopsis

    文献信息:Kim, In-Jung等.  发表于【Plant and Animal Genome XXIII Conference;Plant and Animal Genome】 | 请登录

    简介:A genomic DNA clone encoding carotenoid isomerase (CRTISO) was isolated from Citrus unshiu. It has 12 introns, 13 exons, and 2501 bp of flanking region 5’ of the translational start codon. To identify(X) regulatory elements in the citrus CRTISO (citCRTISO) gene, 2501 bp, 1603 bp and 1037 bp of presumptive promoter DNA sequences and the β-glucuronidase (GUS) coding sequence were fused in pCAMBIA2...

    466. Effects of Different Acetosyringone Concentrations on Transformation of RolABCD Genes in Some Citrus Rootstock Through Agrobacterium rhizogenes

    文献信息:Kacar, Yildiz等.  发表于【Plant and Animal Genome】 | 请登录

    简介:Citrus is an important commodity worldwide and produced in tropical and subtropical regions around the world. Genetic transformation of Citrus via Agrobacterium is a valuable technique for citrus improvement due to difficulties of conventional citrus breeding. In the present study we carried out genetic transformation studies through Agrobacterium rhizogenes ATCC15834 including RolABCD genes to in...

    467. Comparative Protein Production Profiling of (Citrus reticulata blanco) among Tissue Culture Stages by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

    文献信息:K Kazmi, Syeda Khan, Saifullah A Mirbahar, Ameer A Kayani, Hammad等.  发表于【The Natural Products Journal】 | 请登录

    简介:Kinnow mandarin (Citrus reticulata blanco) is the popular fruit crop in the world. In this study 12.5 % sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) method was used to observe comparative protein production pattern in different tissue culture stages of Kinnow mandarin. In this study embryogenic calli, non embryogenic calli, nucellar embryo, in vitro grown plant and seeds ...

    468. Plantlet regeneration via somatic embryogenesis from mature seed of Citrus nobilis variety SoE from SoE NTT

    文献信息:Husain, Indriati Utami, Fitria Nanda Purwito, Agus Husni, Ali Mutaqin, Kikin H Susanto, Slamet等.  2015-11-03 发表于【Horticultural Biotechnology Research/http://sciencefl ora.org/journals/index.php/hbr/】 |  , 请登录

    简介:The aim of this research is a study about direct somatic embryogenesis from mature seed and plantlet regeneration of Citrus nobilis variety SoE. Plant material for somatic embryogenesis is the mature seed of Tangerine SoE. 6-benzylaminopurine (BAP) 3 mg/L supplemented to Murashige and Skoog (MS) modified medium was induced direct somatic embryos (SE) 4 weeks after treatments. MS modified medium wi...

    469. Overexpression of a citrus basic helix-loop-helix transcription factor (CubHLH1), which is homologous to Arabidopsis activation-tagged bri1 suppressor 1 interacting factor genes, modulates carotenoid

    文献信息:Endo, T. Fujii, H. Sugiyama, A. Nakano, M. Nakajima, N. Ikoma, Y. Omura, M. Shimada, T等.  2015-11-30 发表于【Plant science : an international journal of experimental plant biology】 |  , 请登录

    简介:To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homolog...

    470. Early Changes in Host Response to C. Liberibacter asiaticus Infection in Citrus

    文献信息:Chin, Elizabeth Mishchuk, Darya LeVesque, Cynthia Foster, Elizabeth Godfrey, Kristine Slupsky, Carolyn等.  发表于【The FASEB Journal】 | 请登录

    简介:Candidatus Liberibacter asiaticus (CLas), a non-culturable, phloem-limited bacterium, is one of the presumed causative agents of the devastating citrus disease known as Huanglongbing (HLB). HLB was first discovered in Florida in 2005, and has since spread throughout the state. It is now threatening both the California and Texas citrus industries. Diseased trees suffer from discolored leaves and fr...

    471. Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

    文献信息:Cardoso, JC Abdelgalel, AM Chiancone, B Latado, RR Lain, O Testolin, R Germanà, MA等.  发表于【Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology】 | 请登录

    简介:In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can pr...

    472. Somatic Embryogenesis and Plant Regeneration from Juice Vesicles of Mexican Lime (Citrus aurantifolia L.)

    文献信息:Amin, Hossein Shekafandeh, Akhtar等.  发表于【Jordan Journal of Agricultural Sciences】 |  , 请登录

    简介:Callus production, somatic embryogenesis and plant regeneration were achieved from juice vesicles of Mexican lime. First, three types of culture media, Murashige and Skoog (MS), Murashige and Tucker (MT) and Grosser and Gmitter (H+H) and different levels of 2,4-Dicholorophenoxy acetic acid (2,4-D) were used to study their effects on production and proliferation of embryogenic calli from juice vesi...

    473. 冷诱导基因转化柠檬的研究

    文献信息:姜航,胡威,屈汉金,周潇,张凤,邓子牛,龙桂友等.  发表于【湖南农业科学】 | 请登录

    474. Genetic transformation of Citrus sinensis \'Hamlin\' with attacin A driven by a phloem tissue-specific promoter for resistance to Candidatus Liberibacter spp

    文献信息:Tavano, E. C. da R.,Vieira, M. L. C.,Alves-Mourao Filho, F. de A.,Harakava, R.,Mendes, B. M. J.等.  请登录

    简介:Huanglongbing (HLB), associated to the bacteria Candidatus Liberibacter spp. which colonizes the phloem, is currently considered the most destructive citrus disease. Genetic transformation with genes that code for antibacterial peptides associated to phloem-specific promoters is considered an important strategy to control this disease. The objective of this work was to produce transgenic sweet ora...

    475. Efficient in vitro propagation and rooting of adult explants of citrus rootstocks

    文献信息:Tallon C. I.,Cordoba F.,Porras I.,Perez-Tornero O等.  请登录

    简介:The influence of the basal medium and different plant growth regulators on the efficient micropropagation of nodal explants from mature trees of sour orange and \'Cleopatra\' mandarin citrus rootstocks was studied. The preference of the two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed. Several combinations of BA and AD, KIN or GA were made in an attempt to opti...

    476. Radiosensitivity of seeds and nodal segments of citrus rootstocks irradiated in vitro with gamma-rays from 137Cs

    文献信息:Tallon C. I.,Cordoba F.,Porras I.,Perez-Tornero O.等.  请登录

    简介:The first step in a breeding program involving physical mutagenesis by radiation is to evaluate the sensitivity in the plant material. Seeds of Citrus aurantium and C. macrophylla and nodal segments, where the buds were completely removed, from micropropagated explants of macrophylla, were used to determine their radiosensitivity to gamma radiation. A 137Cs source of gamma-rays was used to deliver...

    477. RNA interference against the three Citrus tristeza virus genes encoding silencing suppressors confers complete resistance to the virus in transgenic Mexican lime plants

    文献信息:Soler N.,Plomer M.,Fagoaga C.,Moreno P.,Navarro L.,Flores R.,Pena L.等.  发表于【<Go to ISI>://CABI:20153128821】 | 请登录

    简介:Citrus tristeza virus (CTV), the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins acting at intra- (p23 and p20) and inter-cellular level (p20 and p25) to overcome host antiviral defense. RNA interference (RNAi), an approach based on using dsRNA to trigger RNA silencing, has been widely used for generating transgenic plants resistant aga...

    478. Micro-shoot tip grafting as tool for production of disease free foundation block of Citrus reticulate Blanco \'Khasi\' mandarin

    文献信息:Rakes, Sanabam,Handique P. J.,Sunitibala Huidrom等.  发表于【<Go to ISI>://CABI:20153128813】 | 请登录

    简介:This study was carried out to produce viral disease free scion bank of \'Khasi\' mandarin ( Citrus reticulate Blanco) using four Citrus species namely \'Kachai\' lime ( Citrus jambhiri Lush), Champra maounthabi ( Citrus jambhiri Lush), Solom ( Citrus sps.) and Phouheiree ( Citrus sps) through micrografting techniques as viral disease affects the longevity of citrus orchards and quality of fruits. ...

    479. Chromosome instability in \'Carrizo\' citrange+ Citrus macrophylla somatic hybrids

    文献信息:Pensabene-Bellavia G.,Ruiz M.,Aleza P.,Olivares-Fuster O.,Ollitrault P.,Navarro L.等.  发表于【<Go to ISI>://CABI:20153128818】 | 请登录

    简介:Somatic hybridization by protoplast fusion is an effective technique for citrus rootstock breeding. It theoretically allows the accumulation of all genes of the parents irrespective of their heterozygosity level and therefore the addition of complementary dominant traits. In the present work, protoplasts were isolated from callus of C. macrophylla and from leaves of \'Carrizo\' citrange ( C. sinen...

    480. Somatic Hybridization and Cybridization Via Protoplast Fusion Technology for Citrus Improvement

    文献信息:Omar Ahmad A.,Murata Mayara,Altaf Sadaf,Chase Christine D.,Graham James H.,Grosser Jude W.等.  发表于【In Vitro Cellular & Developmental Biology-Plant】 | 请登录

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