文献信息：Dutt Manjul,Barthe Gary,Irey Michael,Grosser Jude等.  2015-09-23 发表于【PloS one】 |  , 请登录

简介：Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars \'Hamlin\' and \'Valencia\' expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promoter or a phloem specific Arabidopsis SUC2 (AtS...

文献信息：Chiancone, Benedetta,Karasawa, Marines M. Gniech,Gianguzzi, Valeria,Abdelgalel, Ahmed M.,Barany, Ivett,Testillano, Pilar S.,Marinoni, Daniela Torelo,B等.  2015-06-11 发表于【Frontiers in Plant Science| www.frontiersin.org】 |  , 请登录

简介：Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. Anther culture is currently the method of choice for microspore embryogenesis in many crops. However, isolated microspore culture is a better way to investigat...

文献信息：Chunzhen Cheng,Jiawei Yang,Hubin Yan,Xuejun Bei,Yongyan Zhang,Zhiming Lu,Guangyan Zhong等.  发表于【Journal of Integrative Agriculture】 |  , 请登录

简介：The Citrus tristeza virus (CTV) uses 3 silencing suppressor genes, p20, p23 and p25, to resist the attacks from its
<br />Citrus hosts. Inactivating these genes is therefore obviously a potential defensive option in addition to the current
<br />control strategies including aphid management and the use of mild strain cross protection. In this study, we cloned
<br />partial DNA fragments from the t...

文献信息：Al-Zebari, S. M. K.,Al-Brifkany, A. A. A. M.等.  发表于【American Journal of Experimental Agriculture】 | 请登录

简介：This study was conducted during 2012 and 2013 season to investigate the effect of IBA on rooting of citron stem cuttings ( Citrus medica Linnaeus) Corsian cultivar. The cutting referred to the location from which it was taken (tip, medial and bottom). Cuttings were taken and exposed to different IBA doses (0, 500, 1000, 2000 ppm). They were planted in greenhouse in mixed media (1 part peat moss + ...

文献信息：Alvarez-Gerding Ximena,Espinoza Carmen,Inostroza-Blancheteau Claudio,Arce-Johnson Patricio,等.  发表于【Plant Physiology and Biochemistry】 |  , 请登录

简介：Plant stress induced by high salinity has leading to an important reduction in crop, yields. Due to their tropical origin, citrus fruits are highly sensitive to salts. Rootstocks are the root system of fruit trees, regulating ion uptake and transport to the canopy. Therefore,, increasing their salt tolerance could improve the salt tolerance of the fruit tree. For this, we genetically-transformed a...

文献信息：王露,李鹏,苏纯兰,马跃文,颜炼,胡桂兵,秦永华等.  发表于【中国南方果树】 |  , 请登录

简介：以无籽沙糖桔异交种子(无籽沙糖桔×有籽沙糖桔)的实生苗上胚轴为材料,以MT为基本培养基,研究了不同种类的植物生长调节剂及其组合对无籽沙糖桔离体再生的影响,建立了高效的无籽沙糖桔离体再生体系。结果表明:ZT和TDZ不利于无籽沙糖桔不定芽的诱导;单一附加6-BA时,6-BA浓度为0.250mg/L的不定芽诱导率和平均每个外植体不定芽数最高,分别为88.5%和2.7个/外植体;同时附加6-BA与IBA时,以6-BA 0.250mg/L+IBA 0.25mg/L的效果最好,不定芽诱导...

文献信息：李菊,程春振,张永艳,钟云,钟广炎,陆智明等.  发表于【西南大学学报(自然科学版)】 |  , 请登录

简介：用逆转承-cDNA聚合酶链式扩增(RT-PCR)方法 ,分别从克里曼丁橘和拟南芥中克隆出RIN4基因,连接到表达载体pFGC5941,构建了柑橘CcRIN4和拟南芥AtRIN4基因的过表达载体,并成功转入EHA105农杆菌.通过农杆菌介导法将过表达载体转入沙田柚,获得柑橘和拟南芥RIN4基因的转基因沙田柚植株各6株.

文献信息：解栋梁,邹修平,许兰珍,何丹丹,彭爱红,何永睿,陈善春等.  发表于【中国南方果树】 |  , 请登录

简介：为了研究抗菌肽和人溶菌酶对柑桔溃疡病和黄龙病等的协同抗性,利用连接多肽2A编码序列连接抗菌肽cecropin B(CB)和人溶菌酶human lysozyme(HLy)基因,构建了融合基因CB:HLy及其超量表达载体p35S::CB:HLy。利用农杆菌介导法,将该载体转化至锦橙上胚轴。通过GUS化学染色及PCR鉴定,获得转基因植株32株。Real-time PCR证明了融合基因在转基因锦橙中得到了高效表达。本研究为柑桔溃疡病的抗性研究提供了材料。

文献信息：胡建强,吴黎明,蒋迎春,吴述勇,何利刚,廖胜才等.  发表于【农业科技通讯】 |  , 请登录

简介：桃叶橙为湖北秭归地理标志产品,果实具有甜、香、脆的特有品质,为湖北省地方优良特色水果。重点介绍了桃叶橙提纯选优及其种植区域选择、规模化栽植、土肥水管理、整形修剪、花果管理、病虫害防治等优质高效栽培技术。

文献信息：Feng-Quan Tan, Hong Tu, Wu-Jun Liang, Jian-Mei Long, Xiao-Meng Wu, Hong-Yan Zhang and Wen-Wu Guo等.  发表于【Bmc Plant Biology】 |  , 请登录

简介：Background: Polyploidy has often been considered to confer plants a better adaptation to environmental stresses. Tetraploid citrus rootstocks are expected to have stronger stress tolerance than diploid. Plenty of doubled diploid citrus plants were exploited from diploid species for citrus rootstock improvement. However, limited metabolic and molecular information related to tetraploidization is cu...

文献信息：Yahata Masaki，Nukaya Tsunaki，Sudo Miki，Ohta Tomohiro，Yasuda Kiichi，Inagaki Hidehiro，Mukai Hiroo，Harada Hisashi，Takagi Toshihiko，Komatsu Haruki，Kunita等.  发表于【The Horticulture Journal】 |  , 请登录

简介：To obtain basic information about doubled haploid plants in Citrus, in the present study, we investigated the morphological characteristics in doubled haploid induced by colchicine-treated axillary shoot buds of a haploid plant from \'Banpeiyu\' pummelo [C. maxima (Burm.) Merr.]. We also evaluated the reproductive potential of the doubled haploid as a male or a female parent by crossing with some ...

文献信息：Xiao-Meng Wu, Shu-Jun Kou, Yuan-Long Liu, Yan-Ni Fang, Qiang Xu and Wen-Wu Guo等.  发表于【Plant Biotechnology Journal ,BIOTECHNOLOGY & APPLIED MICROBIOLOGY 】 | 请登录

简介：Somatic embryogenesis (SE) is a process of somatic cells becoming dedifferentiated and
<br />generating embryos. SE has been widely used in biotechnology as a powerful way of
<br />regeneration and a model system for studying plant embryogenesis, but the controlling
<br />mechanisms of SE are far from clear. Here, we show the genomewide profiles of miRNAs/siRNAs
<br />and their target genes in non...

文献信息：Dutt, Manjul, Barthe, Gary and Grosser, Jude W.等.  请登录

文献信息：何丹丹， 邹修平， 解栋梁 ，彭爱红 ，许兰珍 ，何永睿， 陈善春等.  2014-07-08 发表于【中国南方果树】 |  , 请登录

简介：绿色荧光蛋白基因(gfp)和新霉素磷酸转移酶基因(nptⅡ)被广泛应用于植物的遗传转化中。为了研究融合标记基因nptⅡ::gfp在锦橙遗传转化中的转化效率,经农杆菌介导法将CaMV35s组成型启动子驱动的nptⅡ::gfp融合标记基因表达载体pNGM和由CaMV35s启动子分别驱动nptⅡ、gfp两个基因的表达载体pNPTⅡ-GF转化至锦橙上胚轴内。GFP绿色荧光检测结果显示,gfp在转nptⅡ::gfp融合基因植株中强烈表达,荧光强度与转非融合nptⅡ和gfp基因植株的表达...

文献信息：Xiao, X., Ma, F., Chen, C., and Guo, W等.  发表于【Plant Cell Tissue & Organ Culture】 | 请登录

简介：An efficient Agrobacterium rhizogenes mediated transformation system of Poncirus trifoliata was developed. The combination of A. rhizogenes MSU440 with epicotyl segments was confirmed to be02a suitable system for genetic transformation in P. trifoliata . About 302weeks after inoculation with bacteria, hairy roots emerged at the cut surfaces of epicotyl segments on Murashige and Tucker selective me...

文献信息：Lei-Ming Sun 1 , Jin-Zhi Zhang &#8727;,1 , Li Mei, Chun-Gen Hu &#8727;等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：For flowering plants, flowering transition is induced in response to endogenous and extrinsic environ-
<br />mental signals. During this process, the members of the APETALA family from MADS-box transcription factors play an important role. Here, to better understand the molecular mechanism of APETALA1 (AP1) in the regulation of citrus flowering, an APETALA1-like homologue gene (PtAP1) was isolated...

文献信息：Ana Rodríguez, Takehiko Shimada, Magdalena Cervera, Berta Alquézar, José Gadea, Aurelio Gómez-Cadenas, Carlos José De Ollas, María Jesús Rodrigo, Lore等.  发表于【Plant Physiol】 |  , 请登录

简介：Terpenoid volatiles are isoprene compounds that are emitted by plants to communicate with the environment. In addition to their function in repelling herbivores and attracting carnivorous predators in green tissues, the presumed primary function of terpenoid volatiles released from mature fruits is the attraction of seed-dispersing animals. Mature oranges (Citrus sinensis) primarily accumulate ter...

文献信息：Li Ding-li, Xiao Xuan, and Guo Wen-wu等.  发表于【Journal of Integrative Agriculture】 | 请登录

简介：Citrus canker, an epidemic quarantine disease caused by Xanthomonas axonopodis pv. citri , has brought a great damage in citrus production worldwide. Herein, a rice PRR (pattern recognition receptor) gene Xa21 together with GUS reporter gene and hygromycin phosphotransferase gene ( HPT ) was introduced into Anliucheng sweet orange ( Citrus sinensis Osbeck) via Agrobacterium -mediated transformatio...

文献信息：Xiaoqing Gong, Jingyan Zhang, Ji-Hong Liu等.  发表于【Plant Physiology and Biochemistry】 |  , 请登录

简介：Exploration of genes functioning in salt tolerance is crucial for generating transgenic plants with enhanced salt tolerance. In this study, we report the isolation and functional characterization of a stress-responsive gene FcSISP from Meiwa kumquat (Fortunella crassifolia). FcSISP encodes a putative protein of 47 amino acids, with a calculated molecular mass of 4.94 kDa and theoretical isoelectri...

文献信息：Hajeri, S. Killiny, N. El-Mohtar, C. Dawson, W. O. Gowda, S.等.  发表于【Journal of biotechnology】 | 请登录

简介：A transient expression vector based on Citrus tristeza virus (CTV) is unusually stable. Because of its stability it is being considered for use in the field to control Huanglongbing (HLB), which is caused by Candidatus Liberibacter asiaticus (CLas) and vectored by Asian citrus psyllid, Diaphorina citri. In the absence of effective control strategies for CLas, emphasis has been on control of D. cit...