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  • 521. 柑橘品种鉴定的SSR标记开发和指纹图谱库构建

    文献信息:李益, 马先锋, 唐浩, 李娜, 江东, 龙桂友, 李大志, 牛英, 韩瑞玺 and 邓子牛等.  发表于【中国农业科学】 | 请登录

    简介:【目的】筛选出一套SSR核心引物,用于构建柑橘品种的DNA指纹图谱库,为柑橘种苗认证、品种登记和植物新品种保护提供技术支撑。【方法】以柑橘属8个主要栽培种类为试材进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测,筛选出多态性丰富、扩增条带稳定的引物对部分参试品种进行PCR扩增,扩增产物通过变性聚丙烯酰胺凝胶电泳进行进一步的引物筛选;筛选得到的引物进行荧光标记,并选用部分柑橘品种进行PCR扩增,扩增产物利用基因分析仪检测,分别计...

    522. Prevention and Control of Taizhou Candidatus Liberibacter asiaticus and Diaphorina citri from 2002 to 2017: A Review

    文献信息:Yu, Jihua, Zhang, Minrong, Zhong, Liequan, Yang, Xiao, Li, Ping, Lu, Lu, Zhang, Ning and Tao, Jian等.  发表于【 Journal of Agriculture】 | 请登录

    简介:This paper mainly reviewed some researches on prevention and control of Candidatus Liberibacter asiaticus and Diaphorina citri in Taizhou from 2002 to 2017. It was found that the population of citrus HLB was distributed evenly in citrus orchards at the early stage of invasion, but subsequently developed in the pattern of aggregation. Accordingly, the population of Diaphorina citri belonged to the ...

    523. Genetic polymorphism analysis of Citrus reticulata Blanco cv. chachiensis Tanaka and its relatives by SCoT molecular marker

    文献信息:Xiu-Li, X. I., Huang, Hai Bo, Lou, Bu Qing, Zhan, Ruo Ting and Wang, Hao Hang等.  发表于【 Chinese Traditional & Herbal Drugs】 | 请登录

    简介: Objective To carry out genetic polymorphism analysis of Citrus reticulata Blanco cv. chachiensis Tanaka and its relatives by using SCoT molecular marker method. Methods Five factors of Mg2+, d NTPs, Taq DNA polymerase, primer, and template DNA concentration were used to screen the suitable SCoT-PCR reaction system for C. reticulata and its relatives by the method of orthogonal design. The optimum...

    524. Mandarin (Citrus reticulata Blanco) Breeding

    文献信息: Usman, Muhammad and Fatima, Bilquees等.  请登录

    简介:The mandarin horticultural group is an important component of world citrus production for the fresh fruit market. This group formerly classified as C. reticulata is highly polymorphic and recent molecular studies have suggested that numerous cultivated mandarins were introgressed by C. maxima (the pummelos). C. maxima and C. reticulata are also the ancestors of sweet and sour oranges,... [Show ful...

    525. Ploidy level of citrus rootstocks affects the carbon and nitrogen metabolism in the leaves of Chromium-stressed Kinnow mandarin plants

    文献信息:Shahid, Muhammad Adnan, Balal, Rashad Mukhtar, Khan, Naeem, Zotarelli, Lincoln, Liu, Guodong, Ghazanfar, Muhammad Usman, Rathinasabapathi, Bala, Matts等.  发表于【Environmental & Experimental Botany】 | 请登录

    简介: This study compared the effects of chromium toxicity on the carbon and nitrogen metabolism in Kinnow mandarin ( Citrus nobilis Lour x Citrus deliciosa Ten) plants grafted on the diploid (2x) and tetraploid (4x) of Poncirus trifoliata [L.], Citrus reshni , and Citrus limonia Osbeck. Plants were grown under controlled conditions and fertigated with complete nutrient solution supplemented with chrom...

    526. Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks

    文献信息: Ruiz, Marta, Pensabene-Bellavia, Giovanni, Quiñones, Ana, García-Lor, Andrés, Morillon, Raphaël, Ollitrault, Patrick, Primo-Millo, Eduardo,等.  发表于【Frontiers in Plant Science】 | 请登录

    简介: Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs. This method allows combining the whole parental genomes in a single genotype, adding complementary dominant characters, regardless of parental heterozygosity. It also contributes to surpass limitations ...

    527. Effect of phosphorus on N, P, K, Mg accumulation and plant growth of different citrus rootstocks

    文献信息: P. F., T. U.等.  发表于【 Applied Ecology & Environmental Research】 | 请登录

    简介: The results of investigation on early evaluation of compatibility between commercial citrus varieties, and Kaffir lime (Citrus hystrix) and Carrizo citrange (C. sinensis Osb. x P. trifoliata L. Raf.) rootstocks, at Mekong Delta Vietnam, during four successive years from 2010 to 2013, reported that out of the tested combinations of the recent commercial citrus varieties, included Da xanh pumelo......

    528. ISSR-Markers Assisted Genetic Diversity Assessment of Acid Lime [Citrus aurantifolia (Christm.) Swingle] Germplasm of Eastern Nepal

    文献信息:Munankarmi, Nabin Narayan, Rana, Neesha, Bhattarai, Tribikram, Shrestha, Ram Lal, Joshi, Bal Krishna, Baral, Bikash and Shrestha, Sangita等.  请登录

    简介:Acid lime [Citrus aurantifolia (Christm.) Swingle] is a fruit crop, enriched with high commercial value and is cultivated in 60 out of 75 districts representing all geographical landscapes of Nepal. Lack of high yielding cultivars is probably one of the main reason for its extremely reduced productivity which warrants a deep understanding of genetic diversity in existing germplasm. Hereby, we aim ...

    529. The citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes

    文献信息: Lu, S. W., Zhang, Y., Zhu, K., Yang, W., Ye, J. L., Chai, L., Xu, Q. and Deng, X.等.  发表于【Plant Physiology】 | 请登录

    简介:Abstract Although remarkable progress has been made towards understanding carotenoid biosynthesis, the mechanisms that regulate the transcription of carotenogenic genes remain poorly understood. Lycopene 脦虏-cyclases (LCYb) are critical enzymes located at the branch point of the carotenoid biosynthetic pathway. Here, we used the promoter sequence of LCYb1 as bait in a yeast one-hybrid screen for ...

    530. miR156-SPLmodules regulate induction of somatic embryogenesis in citrus callus

    文献信息:Long, Jian Mei, Liu, Chao Yang, Feng, Meng Qi, Liu, Yun, Wu, Xiao Meng and Guo, Wen Wu等.  发表于【Journal of Experimental Botany】 | 请登录

    简介:Overexpression of csi-miR156a enhances the somatic embryogenesis capability of citrus through downstream repression ofCsSPL3andCsSPL14. miR156 is a highly conserved plant miRNA and has been extensively studied because of its versatile roles in plant development. Here, we report a novel role of miR156 in regulating somatic embryogenesis (SE) in citrus, one of the most widely cultivated fruit crops ...

    531. Huanglongbing-induced Anatomical Changes in Citrus Fibrous Root Orders

    文献信息:Kumar, Naveen, Kiran, Fnu and Etxeberria, Ed等.  发表于【 Hortscience A Publication of the American Society for Horticultural Science】 | 请登录

    简介:Citrus fibrous roots are vital for absorption and transport of water, nutrients, and other endogenous plant growth regulators. Efficient functioning of these roots in Huanglongbing (HLB)-affected citrus trees is important for their survival. One-year-old \'Valencia\' sweet orange (Citrus sinensis L. Osbeck) trees on Swingle citrumelo were budded with HLB-infected budwood to determine the HLB-induc...

    532. Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

    文献信息:Kumar, Krishan, Gill, Manav Indra Singh and Gosal, Satbir Singh等.  请登录

    简介: Citriculture is widely carried out in both tropical and subtropical regions and is one of the most economically important fruit crops. Although a high genetic variability is present in the genus Citrus and its wild relatives, improvement by conventional breeding is difficult because of the presence of nucellar embryos, a high degree of heterozygosity, and long juvenile periods, in addition to... ...

    533. Effects of δ゛minolevulinic acid dehydratase silencing on the primary and secondary metabolisms of citrus

    文献信息:Citrus production in Florida is the largest in the United States with a value of production of $1.34 billion. Yields of bearing citrus trees affected 等.  请登录

    简介:Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study,... [Sh...

    534. Efficient genetic transformation of sour orange, Citrus aurantium L. using Agrobacterium tumefaciens containing the coat protein gene of Citrus tristeza virus Journal: Plant Gene

    文献信息:Ghaderi, Isa, Sohani, Mohammad Mehdi and Mahmoudi, Ali等.  请登录

    简介: Transgenic Citrus aurantium plants have been obtained by Agrobacterium tumefaciens -mediated gene transfer using seedling-derived epicotyl segments as explants and Basta herbicide as the selective agent. In the present research, hypocotyl, and epicotyls parts of C. aurantium plant were inoculated with A. tumefaciens strain EHA-105. Then, explants were transferred into the regeneration medium and ...

    535. An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange and lemon

    文献信息:Faddetta, Teresa; Abbate, Loredana; Renzone, Giovanni; Palumbo Piccionello, Antonio; Maggio, Antonella; Oddo, Elisabetta; Scaloni, Andrea; Puglia, Ann等.  发表于【Plant Molecular Biology】 | 请登录

    简介:KEY MESSAGE: Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded t...

    536. Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene

    文献信息:Kobayashi Adilson K., Vieira Luiz Gonzaga E., Bespalhok Filho Joao Carlos, Leite Rui Pereira Jr., Pereira Luiz Filipe P., Molinari Hugo Bruno, Marques等.  发表于【European Journal of Plant Pathology】 | 请登录

    简介:Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the...

    537. Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

    文献信息:Peng A, Chen S, Lei T, Xu L, He Y, Wu L, Yao L, Zou X等.  2017-12-01 发表于【Plant Biotechnology Journal】 | 请登录

    简介:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR/Cas9-targeted modification of the susceptibility gene CsLOB1 pro...

    538. 柑橘转录因子CsCBF1和PtrNAC72在调控精氨酸脱羧酶基因表达及抗逆中的功能鉴定

    文献信息: 吴昊等.  请登录

    简介:柑橘是世界上栽培最广、产量最高的水果作物,其中甜橙所占的比例最大,年产量约为柑橘类水果总产量的60%。甜橙喜温不耐寒,因此低温是限制柑橘产量的主要环境因素之一。多胺是含有两个或两个以上氨基的脂肪族化合物,参与植物生长发育、组织分化、果实成熟与衰老以及抵抗生物和非生物胁迫等重要生理过程,在一定程度内,多胺在细胞中的积累量越多,其胁迫抗性越强。而在逆境胁迫下多胺合成酶基因表达量的上调是多胺在细胞中积累的前提,因此...

    539. Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing

    文献信息:Xu, X., Xu, X., Zhou, Y., Zeng, S. and Kong, W.等.  发表于【Plos One】 | 请登录

    简介:Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts. MicroRNAs (miRNAs) play important roles in plant growth, development and stress responses. However, the underlying mechanism of miRNAs involved in the protoplast totipotency remains uncle...

    540. Optimization of an in vitro Regeneration Protocol for Rough Lemon Rootstock (Citrus jambhiri L.) via Direct Organogenesis

    文献信息:Taye, Molla Gereme, Debesay, Brhanu, Tesfahun, Yikunoamilak and Brhanu, Assefa等.  请登录

    简介:Optimization of an in vitro Regeneration Protocol for Rough Lemon Rootstock (Citrus jambhiri L.) via Direct Organogenesis Abstract.

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