文献信息：姚利晓,何永睿,邹修平,雷天刚,许兰珍,彭爱红,陈善春等.  发表于【果树学报】 |  , 请登录

简介：由于柑橘具有童期长、基因高度杂合、多胚性、远缘杂交不亲和及性器官败育等生物学特性,在很大程度上限制了传统育种的发展,基因工程育种能高效地对植物进行定向改良,将成为柑橘定向改良育种研究工作的重要手段。近十余年来,已成功利用农杆菌侵染法将外源基因转入到越来越多的柑橘品种,并建立柑橘成年态材料的遗传转化体系和标记基因删除系统。对近年来柑橘成年态材料转化、标记基因的更替和转基因柑橘安全性研究等方面的成果进行了总...

文献信息：杨莉,胡威,谢玉明,李义,邓子牛等.  发表于【中国园艺学会2013年学术年会】 |  , 请登录

简介：截至2012年,全球转基因作物种植面积达1.7亿hm~2,转基因作物带来巨大经济和社会效益的同时也引起了人们对转基因食品和环境安全的担忧,阻碍转基因技术的进一步发展。外源基因清除技术‘Gene-deletor'由美国康涅狄格大学李义教授发明,此技术利用器官或组织特异启动子、重组酶及融合识别位点构建基因表达元件,能将转基因植物中的全部外源基因在完成其功能作用后自动地从花粉、种子和果实中彻底清除。在本研究中,将外源基因清除技术应用...

文献信息：Stover E, Stange R R, McCollum T G, Jaynes J, Irey M, Mirkov E等.  发表于【J. Am. Soc. Hortic. Sci.】 | 请登录

简介：Huanglongbing {HLB [associated with Candidatus Liberibacter sp. (CLas)]} and asiatic citrus canker {ACC [causal organism Xanthomonas citri ssp. citri (XCC)]} are bacterial diseases that seriously threaten sustainability of the Florida citrus (Citrus sp.) industry. Sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) are highly susceptible to ACC and improvement through conventional bree...

文献信息：Donmez, D.,Simsek, O.,Izgu, T.,Kacar, Y. A.,Mendi, Y. Y.等.  发表于【The Scientific World Journal】 |  , 请登录

简介：Citrus is one of the world's important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. Citrus transformation has now been achieved in ...

文献信息：Del Bosco, S. F.,Abbate, L.,Tusa, N.,Strano, T.,Renda, A.,Ruberto, G.等.  发表于【Food Research International】 |  , 请登录

简介：The peel essential oil profiles of an interspecific allotetraploid somatic hybrid, achieved by protoplast fusion of the 'Valencia' sweet orange (Citrus sinensis L. Osbeck) and 'Femminello' lemon (Citrus limon L. Burm), and three sexual hybrids obtained by back crosses between Femminello lemon and the allotetraploid somatic hybrid (Valencia + Femminello), have been studied by gas chromatography (GC...

文献信息：Benyon, L. S.,Stover, E.,Bowman, K. D.,Niedz, R.,Shatters, R. G.,Zale, J., Belknap, W. 等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 | 请登录

简介：Transgenic solutions are being widely explored to develop huanglongbing (HLB) resistance in citrus. A critical component of a transgenic construct is the promoter, which determines tissue specificity and level of target gene expression. This study compares the characteristics of five promoters regulating the beta-glucuronidase (GUS) reporter gene in the trifoliate hybrid rootstock US-802. Two of t...

文献信息：Tallon, C. I.,Porras, I.,Perez-Tornero, O.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：A simple and efficient protocol for obtaining organogenesis from mature nodal explants of Citrus macrophylla (alemow) and Citrus aurantium (sour orange) has been developed by optimizing the concentrations of the plant growth regulators, the incubation conditions, the basal medium and by the choice of explant. In order to optimize the plant growth regulator balance, explants were cultured in the re...

文献信息：Zou, X. P.,Peng, A. H.,Xu, L. Z.,Liu, X. F.,Chen, S. C.,Lei, T. G.,Yao, L. X.,He, Y. R.,等.  发表于【Plant Cell Reports】 |  , 请登录

简介：A highly efficient Cre-mediated deletion system, offering a good alternative for producing marker-free transgenic plants that will relieve public concerns regarding GMOs, was first developed in citrus. The presence of marker genes in genetically modified crops raises public concerns regarding their safety. The removal of marker genes can prevent the risk of their flow into the environment and hast...

文献信息：刘小丰,彭爱红,许兰珍，邹修平，朱世平，赵晓春，陈善春等.   , 请登录

简介：早期、无损伤、易于操作的阳性芽检测技术,对提高柑橘遗传转化效率是十分有利的。本文设计和构建了nptⅡ::mgfp5融合基因,通过锦橙上胚轴转化体系研究其在柑橘遗传转化中的作用。nptⅡ::mgfp5融合基因全长1 578 bp,编码525个氨基酸,作为表达载体p1301NG的选择标记和报告基因。通过农杆菌介导法转化锦橙上胚轴,再生培养1周后用体式荧光显微镜观察外植体的再生情况。在再生培养第9天时即观察到GFP荧光再生芽的形成,再生培养后10~15 d是...

文献信息：许兰珍，何永睿，彭爱红，雷天刚，刘小丰，邹修平，姚利晓，陈善春等.  2013-07-01 发表于【农业生物技术学报】 | 请登录

简介：为进一步明确外源基因在转基因柑橘无性繁殖后代中的遗传稳定性及目标性状表现,本研究以转双价抗菌肽基因(Shiva A-cecropin B)纽荷尔脐橙(Citrus Sinensis Osbeck)无性繁殖后代植株为材料,通过PCR扩增,Southern杂交和实时定量PCR检测,以及温室抗病性评价分析等,对抗菌肽Shiva A基因在转基因柑橘后代株系中的遗传稳定性进行了研究。结果表明,外源抗菌肽Shiva A基因在转基因及其无性后代中能够稳定整合与表达。Southern杂交分析表明,S...

文献信息：张凌云,邓赞,安静等.   , 请登录

简介：富含脯氨酸蛋白4(PRP4,proline-rich protein 4)是一类响应胁迫并在细胞发育过程中起作用一种细胞壁蛋白。用RT-PCR克隆柑橘CsPRP4基因的正、反义cDNA序列,分别连接到T载体pMD–19T上;经限制性核酸内切酶2次双酶切,将CsPRP4基因的正、反义片段定向连接至双元质粒pFGC5941查耳酮合成酶A(CHSA)内含子的两端,构成反向重复序列;经菌液PCR和测序验证,成功构建了CsPRP4基因的RNA干涉载体;经农杆菌介导法转化柑橘,转基因植株具有明显不同于对...

文献信息：张文，胡威，张新宇，周敏，蒋巧巧，邓子牛，李大志等.   , 请登录

简介：【目的】为了获得沙田柚×枸橼杂交苗,【方法】以沙田柚为母本,枸橼为父本进行杂交育种,利用胚抢救技术获得杂种后代,并利用SRAP分子标记对亲本和子代的DNA进行扩增,分析亲本与杂交种之间扩增条带的差异。【结果】结果表明,最终获得27株杂交后代,引物组合Me6-Em5和Me10-Em12可鉴定其中5株为有性后代。【结论】MT+GA3(1 mg.L-1)培养基适合沙田柚×枸橼杂交胚抢救,利用SRAP标记可鉴定出杂交子代,可为以后利用BSA法构建抗感病池寻找柑橘...

文献信息：郑蓓蓓，谢宗周，郭文武等.   , 请登录

简介：采用高效液相色谱测定柑橘种间体细胞杂种[‘朋娜’脐橙(Citrussinensis Osbeck)+粗柠檬(C.jambhiri Lush)]和其两个融合亲本果实的类胡萝卜素含量,气相色谱测定可溶性糖及有机酸含量,并检测代谢过程中关键基因的表达,比较分析体细胞杂种果实品质遗传及基因表达特点。结果表明,体细胞杂种类胡萝卜素成分及含量均偏向粗柠檬亲本,柠檬酸、苹果酸积累量也偏向粗柠檬;而蔗糖含量处在中亲值;实时定量PCR技术检测类胡萝卜素代谢途径中的7个...

文献信息：SD Singer，KD Cox等.  发表于【Journal of Plant Biochemistry and Biotechnology】 | 请登录

简介：Membrane-localized H + -symporting sucrose transporters (SUC or SUT proteins) are involved in sucrose loading into the phloem of source tissues and sucrose uptake into sink tissues, which are essential events in the growth and development of higher plants. While many of these sucrose transporters are localized in the phloem, others function in sink tissues. In an attempt to gain insight into which...

文献信息：Zhijian T. Li , Frederick G. Gmitter Jr. ,Jude W. Grosser , Chunxian Chen , Dennis J. Gray等.  发表于【Tree Genetics & Genomes】 | 请登录

简介：MYB transcription factors play an important role in the activation and regulation of anthocyanin biosynthesis. Many such transcription factor genes from various plant species were well characterized at the molecular level. However, none were reported in Citrus . Here, we demonstrated the isolation, molecular characterization, and functional evaluation of a large number of Citrus genes belonging to...

文献信息：Eun-Ji Koh , Lijuan Zhou , Donna S. Williams , Jiyoung Park ,Ningyuan Ding , Yong-Ping Duan , Byung-Ho Kang等.  发表于【Protoplasma】 |  , 请登录

简介：Huanglongbing (HLB) is a destructive disease of citrus trees caused by phloem-limited bacteria, Candidatus Liberibacter spp. One of the early microscopic manifestations of HLB is excessive starch accumulation in leaf chloroplasts. We hypothesize that the causative bacteria in the phloem may intervene photoassimilate export, causing the starch to over-accumulate. We examined citrus leaf phloem cell...

文献信息：Soriano L, Mourao FDA, Camargo LEA, Cristofani-Yaly M, Latado RR, Pacheco CD, Azevedo FA, Mendes BMJ.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC), 】 | 请登录

简介：Protoplast fusion between sweet orange and mandarin/mandarin hybrids scion cultivars was performed following the model "diploid embryogenic callus protoplast + diploid mesophyll-derived protoplast". Protoplasts were isolated from embryogenic calli of 'Pera' and 'Westin' sweet orange cultivars (Citrus sinensis) and from young leaves of 'Fremont', Nules', and 'Thomas' mandarins (C. reticulata), and ...

文献信息：Abbate L, Tusa N, Del Bosco SF, Strano T, Ruberto G, Renda A.等.  发表于【Food Research International】 |  , 请登录

简介：Three new somatic hybrids, namely an allotetraploid hybrid and two cybrids (2n and 4n), have been obtained by protoplast fusion of 'Valencia' sweet orange (Citrus sinensis L. Osbeck) + 'Femminello' lemon (C. limon L. Burm.). The chemical composition of the essential oils of the hybrids and their parents has been studied by gas chromatography (GC) combined with a flame ionisation detector (FID) and...

文献信息：Aleza P, Juarez J, Cuenca J, Ollitrault P, Navarro L.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. The recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. Triploid plants can be recovered by 2x x 4x sexual ...

文献信息：解凯东,王惠芹,王晓培,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：我国不仅是柑橘的起源地,而且柑橘种植面积和产量均居世界首位。但我国大约40%的柑橘有种籽,影响了其果实品质和商品价值。培育品质优良、食用方便、竞争力强的无籽品种一直是柑橘育种的重要目标。三倍体由于减数分裂不正常,配子败育,是天然的无籽类型。而培育三倍体的方法中,以四倍体与二倍体的倍性杂交最有效。本研究于2009-2011年连续3年,以HB柚、华农红柚、高斑柚、本地早橘、红橘、秋辉橘、克里曼丁橘、鄂柑1号椪柑、清见橘橙、...