文献信息：Martinez-Godoy M. A., Mauri N., Juarez J., Marques M. C., Santiago J., Forment J., Gadea J.等.  发表于【BMC Genomics】 | 请登录

简介：Background: Understanding of genetic elements that contribute to key aspects of citrus biology will impact future improvements in this economically important crop. Global gene expression analysis demands microarray platforms with a high genome coverage. In the last years, genomewide EST collections have been generated in citrus, opening the possibility to create new tools for functional genomics i...

文献信息：Levy, A., Erlanger, M., Rosenthal, M. and Epel, B. L.等.  请登录

简介：Plasmodesmal conductivity is regulated in part by callose turnover, which is hypothesized to be determined by beta-1,3-glucan synthase versus glucanase activities. A proteomic analysis of an Arabidopsis thaliana plasmodesmata (Pd)-rich fraction identified a beta-1,3-glucanase as present in this fraction. The protein encoded by the putative plasmodesmal associated protein (ppap) gene, termed AtBG_p...

文献信息：Orbovic, V., Pasquali, G., Grosser, J. W.等.  发表于【Acta Horticulturae】 |  , 请登录

文献信息：Mourao, F. D. A.,Pio, R.,Mendes, B. M. J.,de Azevedo, F. A.,Schinor, E. H., Entelmann, F. A., Alves, A. S. R. , Cantuarias-Aviles, T. E. 等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Somatic hybridization is a biotechnology tool that can be used in citrus breeding programs to produce somatic hybrids with the complete genetic combination of both parents. The goal of this work was to test the reaction of citrus somatic hybrids that may be useful as rootstocks to trunk and root infections caused by Phytophthora nicotianae van Breda de Haan (P parasitica Dastur) and to citrus tris...

文献信息：Tan, M. L.,Song, J. K.,Deng, X. X.等.   , 请登录

简介：Intergeneric sexual hybridizations were conducted between two genera of Rutaceae, with Satsuma mandarin (Citrus unshiu Marc) and Red tangerine (C. reticulata Blanco) as maternal parents, and Poncirus trifoliata (L.) Raf as the paternal parent, in an effort to generate hybrid populations for both molecular mapping and rootstock breeding. Embryo rescue is important for citrus sexual breeding because...

文献信息：Pavan, Alexandra,Calixto, Marcia Cristina,Cardoso, Suane Coutinho, Januzzi Mendes, Beatriz Madalena, Bergamin Filho, Armando, Spotti Lopes, Joao等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Asiatic citrus canker (ACC), caused by Xanthomonas axonopodis Starr & Garces pv. citri (Hasse) Vauterin et al., and citrus variegated chlorosis (CVC), caused by Xylella fastidiosa Wells et al., are considered the main diseases affecting sweet orange scion varieties in Brazil. Among commercial varieties, mandarins and tangerines are recognized as tolerant to these pathogens. We report herein the pr...

文献信息：Olivares-Fuster, O.,Hernandez-Garrido, M.,Guerri, J.,Navarro, L.等.  发表于【Tree Physiology】 | 请登录

简介：Unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. In addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important t...

文献信息：Guo, W. W.,Wu, R. C.,Cheng, Y. J.,Deng, X. X.等.   , 请登录

简介：Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis x P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slow...

文献信息：Grosser, J. W.,Chandler, J. L.,Duncan, L. W.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Sting nematode (Belonolaimus longicaudatus Rau) has become a primary factor limiting citrus production in localized regions of the central Florida sandridge citrus production area, making the development of resistant rootstocks a new breeding objective. In efforts to develop a replacement rootstock for the widely adapted sour orange, our focus has been on somatic hybridization of selected mandarin...

文献信息：Cai, X. D.,Fu, J.,Deng, X. X.,Guo, W. W.等.  发表于【Plant Cell, Tissue and Organ Culture】 |  , 请登录

简介：Cytoplasmic male sterility (CMS) is known to be controlled by mitochondrial genome in higher plants including Satsuma mandarin (Citrus unshiu Marc.). Citrus symmetric fusion experiments often produce diploid cybrids possessing nuclear DNA from the mesophyll parent and mitochondrial DNA (mtDNA) from the embryogenic callus parent. Therefore, it is possible to transfer CMS from Satsuma mandarin as ca...

文献信息：刘继红等.  发表于【湖北省植物生理学会第十五次学术研讨会】 |  , 请登录

简介：柑桔是一种重要的果树,由于具有多胚性、遗传高度杂合和童期长等特点,传统育种技术在柑桔品种改良上的进程受到阻碍,采用生物技术手段进行遗传改良具有重要的意义。原生质体融合是克服上述困难创造新种质的重要手段,自1985年获得首例柑桔体细胞杂种以来,已得到300余例多种倍性的柑桔体细胞杂种,柑桔体细胞杂种的鉴定主要采用形态学、细胞学和分子标记完成,并且对部分体细胞杂种的核质组成进行了详细分析,体细胞杂种的应用主要表现在接...

文献信息：郭文武,陈春丽,邓于洋,邓秀新等.  发表于【园艺学报】 |  , 请登录

简介：二倍体锦橙+种间四倍体体细胞杂种HR(哈姆林甜橙+粗柠檬)的种间六倍体体细胞杂种植株移入温室7年后(即2004年),有1株六倍体首次开花,花粉染色活力40.8%,花粉萌发率3.7%;2006年该植株再次开花,花粉染色活力为20.3%。由于自然界中没有柑橘六倍体,本研究是人工的方法创造出可育的柑橘六倍体体细胞杂种植株的首例报道。

文献信息：郭文武,蔡小东,付婧,谭彬,邓秀新等.  发表于【全国“植物生物技术及其产业化”研讨会】 |  , 请登录

简介：果实无核是鲜食柑橘品质优良的重要标志,我国有近一半的柑橘为有核类型,采用细胞工程进行我国特色有核柑橘品种的无核化遗传改良具有重要意义。我们建立了成熟的柑橘原生质体操作技术,获得了30余例种间、属间柑橘体细胞杂种/胞质杂种；将优良体细胞杂种的花粉与我国特色地方良种杂交,结合胚挽救,创造了10余个组合240余棵三倍体植株；

文献信息：郭文武,蔡小东,付婧,谭彬,邓秀新等.  发表于【中国园艺学会十届二次理事会暨学术研讨会】 |  , 请登录

简介：果实无核是鲜食柑橘品质优良的重要标志,我国有近一半的柑橘为有核类型,采用细胞工程进行我国特色有核柑橘品种的无核化遗传改良具有重要意义。我们建立了成熟的柑橘原生质体操作技术,获得了30余例种间、属间柑橘体细胞杂种,胞质杂种;将优良体细胞杂种的花粉与我国

文献信息：伊华林,邓秀新等.  发表于【中国科学(C辑:生命科学)】 |  , 请登录

简介：筛选31条谱带清晰、多态性强及重复性好的10bp引物,运用RAPD技术对以异源四倍体柑橘体细胞杂种(哈姆林甜橙(Citrus sinensis Osbeck)+粗柠檬(C.jambhiri Luss))为父本与二倍体单胚类型宜本杂4号(华农本地早橘(C.reticulata Blanco)×宜昌橙(C.ichangensis Swingle))杂交获得的杂种后代进行遗传分析.χ2测验表明:杂交父本与母本的特异标记总体上是随机传递给后代的,父本即柑橘异源四倍体体细胞杂种融合双亲的单个特异标记也是随机传递...

文献信息：吴礽超,赵小龙等.  发表于【广西园艺】 |  , 请登录

简介：柑橘是世界第一大水果,世界三大贸易农产品之一,其新品种的选育一直是育种工作者奋斗的目标。文章介绍了在生物技术快速发展的今天,广大科研工作者在柑橘育种上应用的新技术,包括组织培养育种技术,体细胞杂交技术,转基因育种技术等。

文献信息：刘霞等.   , 请登录

简介：砧木是果树生长的基础，砧木的种类对果树地上部的生长与结果等诸多性状具有重要的作用，而根系中导管等的结构性状在一定程度上决定了它的吸收与输导能力，从而影响到地上部的生长。本试验采用石蜡切片法和组织离析法分别对几种柑橘的普通砧木和体细胞杂种砧木根系木质部和导管分子的解剖结构进行了比较，并对导管分子的形态进行了定量分析。经过对根系横切结构、导管分子形态和数量性状的分析，结果如下： 1．砧木根系横切结构 (...

文献信息：谈安群等.  发表于【安徽农学通报】 |  , 请登录

简介：快速发展的分子标记技术在柑橘种质资源研究与育种实践中得到了广泛应用。本文简要阐述了分子标记的主要类型、原理、特点及其在柑橘种质资源方面的研究,并对当前分子标记存在的问题及以后的发展前景进行了分析。

文献信息：谢玉明等.  请登录

简介：柑橘是世界第一大水果，年产量超过1.1亿吨，中国是柑橘生产大国(面积第一，产量第二)。世界各国都非常重视柑橘的新品种选育，常规方法的选育中种工作一直在开展，并不断获得新的推广品种。但是，由于柑橘自身的一些生物学特性，如实生苗童期长，遗传背景复杂，杂合程度高，具有多胚性等的影响，常规育种常遇到一些障碍，影响育种进程。随着现代生物技术的迅速发展和遗传转化技术的日益完善，将外源基因导入到柑橘中，定向改良现有品...

文献信息：刘佳等.  请登录

简介：柑橘是世界上具有重要经济价值的水果。生产中容易受到冷害和热害等环境胁迫的影响。鉴于柑橘童期长、遗传上不亲和、存在珠心胚干扰等问题，遗传研究困难，杂交育种在短期内难以实现定向育种目标。植物基因工程的发展为柑橘育种提供了一条新途径。 本研究以默科特橘橙转MdSPDS1基因的单克隆细胞系愈伤组织及金柑转基因植株为试材，研究了温度逆境下转基因材料抗性相关生理指标，以评价外源基因对提高柑橘抗逆性的作用与应用前景。主...