文献信息：Kim IJ, Lee J, Han JA, Kim CS, Hur Y.等.  发表于【CANADIAN JOURNAL OF PLANT SCIENCE】 | 请登录

文献信息：王会全, 曾黎辉, 吴少华, 徐海峰.等.  发表于【生物技术通报】 |  , 请登录

简介：To inoculate A．tumefaciens into the embryonic apical meristerm of the soaked seeds，a region on the seed suiface where a shoot would later emerge was pierced twice up to a depth of about 1 mm with a needle dipped in the A．tumefaciens inoculum．Germinated seedings were transplanted．One transgenic plantlet was obtained，which was confirmed by PCR assay and PCR-Southern blot．The transformation ra...

文献信息：Rodríguez A, San Andrés V, Cervera M, Redondo A, Alquézar B, Shimada T, Gadea J, Rodrigo MJ, Zacarías L, Palou L, López MM, Castañera P, Pe&nti等.  发表于【Plant Physiology】 |  , 请登录

简介：Plants use volatile terpene compounds as odor cues for communicating with the environment. Fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. We have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. Because limonene represents up to 97% of the total volatiles in orange (Citr...

文献信息：Favero P, Mourao FDA, Stipp LCL, Mendes BMJ.等.  发表于【Acta Physiologiae Plantarum】 |  , 请登录

简介：The difficulty in adult tissue genetic transformation in woody species is still an obstacle to be overcome, including in most sweet orange cultivars of the Brazilian citrus industry. This work reports that, after in vitro culture adjustments, transgenic adventitious buds of ‘Hamlin’, ‘Pe?ra’, and ‘Valencia’ sweet oranges (Citrus sinensis L. Osbeck) were recovered using adult material as expl...

文献信息：Campos MKF, Carvalho K, Souza FS, Marur CJ, Pereira LFP, Filo JCB, Vieira LGE.等.  发表于【Environmental and Experimental Botany】 |  , 请登录

简介：In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic ‘Swingle’ citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic ra...

文献信息：刘小丰, 彭爱红, 许兰珍, 雷天刚, 姚利晓, 何永睿, 陈善春.等.  发表于【果树学报】 |  , 请登录

简介：Iron is an essential trace element for plants，and it is well known that iron deficiency is a significant global problem.Fruit trees are perennial plants，have been more seriously affected by iron deficiency.Breeding for tolerance to iron deficiency using conventional breeding and selection techniques is very time-consuming，laborious，and inefficient.Genetically modifying fruit trees to improve i...

文献信息：Sorkina A, Bardosh G, Liu YZ, Fridman I, Schlizerman L, Zur N, Or E, Goldschmidt EE, Blumwald E, Sadka A.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was detected in vegetative organs. These expression patterns suggested that Cl111 is...

文献信息：Reyes CA, Zanek MC, Velazquez K, Costa N, Plata MI, Garcia ML.等.  发表于【Journal of Phytopathology】 |  , 请登录

简介：Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees, psorosis A (PsA) and psorosis B (PsB). PsA protects against the severe ef...

文献信息：Ruiz-Ruiz S, Navarro B, Gisel A, Peña L, Navarro L, Moreno P, Di Serio F, Flores R.等.  发表于【Plant Molecular Biology】 | 请登录

简介：To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime...

文献信息：La Malfa S, Distefano G, Domina F, Nicolosi E, Toscano V, Gentile A.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Grosser JW, Dutt M, Omar A, Orbovic V, Barthe G.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：袁飞荣, 严佳文, 罗坤, 王晓君, 邓子牛.等.  请登录

简介：rolABC genes were cloned from T-DNA of Ri plasmid in Agrobactecium rhizogenes, apical dominance weakened, lateral branehes increased. internode shortened and some other special charaeters induced by transformation these genes into plants. It's very important to understand the molecular process of plant growth and development and charaeter formation, real一time PCR is high efficient and convenient ...

文献信息：Tan B, Guo WW.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Roussosi PA, Dimitriou G, Voloudakis AE.等.  发表于【SPANISH JOURNAL OF AGRICULTURAL RESEARCH,】 | 请登录

文献信息：Dambier D, Benyahia H, Pensabene-Bellavia G, Aka Kaçar Y, Froelicher Y, Belfalah Z, Lhou B, Handaji N, Printz B, Morillon R, Yesiloglu T, Navar等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for alternative CTV-resistant rootstocks that also withstand the other constraints is now considered an...

文献信息：Miyata LY, Mourão Filho FAA, Scarpare Filho JA, Zambon F, Bassan MM, Mendes BMJ, Harakava R等.  发表于【Rev. Bras. Frutic., Jaboticabal - SP,】 |  , 请登录

简介：Genetic transformation is considered an important tool to be applied in citrus genetic improvement. However, transformation efficiency may vary according to several factors, including the genetic construction utilized. This study was carried out to evaluate the efficiency of genetic transformation of ‘Carrizo’ citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck], containing two...

文献信息：Loeza-Kuk E, Gutierrez-Espinosa MA, Ochoa-Martinez DL, Villegas-Monter A, Mora-Aguilera G, Palacios-Torres EC, Perez-Molphe-Balch E.等.  发表于【Publicado como ART?CULO en Agrociencia】 |  , 请登录

简介：In citrus tristeza management tolerant rootstocks have been used, but have less plasticity than C. aurantium, and crossprotection sometimes is overcome by more severe variants. An alternative may be transgenic plants with pathogenderived resistance PDR. To evaluate the conferred PDR with transgenesis plants of Citrus paradisi and C. aurantifolia, transformed via Agrobacterium tumefaciens and A. rh...

文献信息：Fagoaga C, Pensabene-Bellavia G, Moreno P, Navarro L, Flores R, Pena L等.  发表于【Molecular Plant Pathology】 |  , 请登录

简介：Citrus tristeza virus (CTV), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling CTV symptoms when expressed ectopically in transgenic citrus hosts. In this article, the effect of p23 ectopic expression on virus infection was examined in sweet oran...

文献信息：Hasegawa S, Suhayda C, Omura M, Berhow M等.  发表于【Biotechnology for improved foods and flavors】 | 请登录

文献信息：袁飞荣， 严佳文， 罗坤， Alessandra ，Gentile， 王晓君， 刘东波， 邓子牛等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：以转rolABC基因枳橙B、D、E系及野生植株为材料，制备标准品，采用SYBR Green I染料，构建rol基
<br />因和β-actin基因的双标准曲线，校正引物扩增效率后，用 2－&#8710;&#8710;Ct法对rol基因在嫩茎、嫩叶、功能叶、树皮和根中的mRNA水平进行相对定量，建立适合于rolA、rolB、rolC基因实时荧光定量RT-PCR分析方法．初步认为rolC基因在嫩茎、嫩叶、功能叶和树皮中表达量最高；其次是rolA基因，主要在嫩茎中表达；rolB基因表达量最低...