文献信息：Omar AA, Dekkers MG, Graham JH, Grosser JW.等.  发表于【Biotechnology Progress】 |  , 请登录

简介：Quantitative real-time PCR(qRT-PCR) was adapted to estimate transgene copy number of the rice Xa21 gene in transgenic citrus plants. This system used TaqMan qRT-PCR and the endogenous citrus gene encoding for lipid tansfer protein(LTP). Transgenic 'Hamlin' sweet orange plants were generated using two different protoplast-GFP transformation systems: cotransformation and single plasmid transformatio...

文献信息：Terol J, Naranjo MA, Ollitrault P, Talon M.等.  发表于【BMC Genomics】 |  , 请登录

简介：Background: Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of ge...

文献信息：Febres VJ, Lee RF, Moore GA等.  发表于【Plant Cell Reports】 | 请登录

简介：Grapefruit (Citrus paradisi) transgenic plants transformed with a variety of constructs derived from the Citrus tristeza virus (CTV) genome were tested for their resistance to the virus. Most transgenic lines were susceptible (27 lines), a few were partially resistant (6 lines) and only one line, transformed with the 30 end of CTV was resistant. Transgene expression levels and siRNA accumulation w...

文献信息：Cervera M, Navarro A, Navarro L, Peña L.等.  发表于【Tree Physiology】 | 请登录

简介：Genetic transformation of mature trees is difficult because adult tissues are recalcitrant to Agrobacterium tumefaciens infection and transformation and because transgenic mature events are less competent for regeneration. We have shown that reinvigoration allows manipulation of the vegetative phase to increase the potential for transformation and regeneration without loss of competence for flower...

文献信息：Mendes AFD, Cidade LC, Manzoli GN, Otoni WC, Soares WD, Costa MGC.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotyp...

文献信息：Guo WW, Duan YX, Li DL, Liu X, Tan B, Cai XD, Grosser JW, Deng XX.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Luro FL, Costantino G, Terol J, Argout X, Allario T, Wincker P, Talon M, Ollitrault P, Morillon R.等.  发表于【BMC Genomics】 |  , 请登录

简介：Background: During the last decade, numerous microsatellite markers were developed for genotyping and to identify closely related plant genotypes. In citrus, previously developed microsatellite markers were arisen from genomic libraries and more often located in non coding DNA sequences. To optimize the use of these EST-SSRs as genetic markers in genome mapping programs and citrus systematic analy...

文献信息：张觅, 罗小英, 白文钦, 李怡然, 侯磊, 裴炎, 李忠旺, 李德谋.等.  发表于【园艺学报】 |  , 请登录

简介：Malate, ubiquitously existing in plan,t plays a crucial role in variousmetabolic pathways, including respiration, nitrogen fixation, phosphorus acquisition, and aluminum tolerance. Malate dehydrogenase(MDH) catalyzes the interconversion ofoxaloacetate andmalate. Previous results suggested that overexpressing MDHgene transgenic plants elevated tolerance to aluminum toxicity.Citrus junos, one ofmain...

文献信息：Omar A, Dutt M, Barthe G, Orbovic V, Grosser J.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Omar AA, Grosser JW.等.  发表于【Plant Science】 |  , 请登录

简介：In order to improve transformation efficiency in citrus two green fluorescent protein (GFP) constructions, an endoplasmic reticulum targeted (GFP-ER) and cytoplasmic targeted (Cy-GFP), were used to study expression and stability of GFP in transgenic plants. Results from citrus protoplast-GFP transformation showed that GFP-ER had brighter fluorescence than the Cy-GFP. Although both of these constru...

文献信息：Andreote FD, Rossetto PB, Souza LCA, Marcon J, Maccheroni W, Azevedo JL, Araujo WL.等.  发表于【Journal of Basic Microbiology】 | 请登录

简介：Harmless bacteria inhabiting inner plant tissues are termed endophytes. Population fluctuations in the endophytic bacterium Pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. The results demonstrated that populations of P. agglomerans fluctuated in Citrus reticulata but not C. sinensis. A cryptic plasmid pPA3.0 (2.9 kb) was identi...

文献信息：M. L. P. Oliveira, V. J. Febres, M. G. C. Costa, G. A. Moore and W. C. Otoni等.  发表于【Plant Cell Reports】 | 请登录

简介：An improved method for the Agrobacterium infiltration of epicotyl segments of ‘Pineapple’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Swingle’ citrumelo [Citrus paradisi Macf. X Poncirus trifoliata (L.) Raf.] was developed in order to increase transformation frequency. Sonication-assisted Agrobacterium-mediated transformation (SAAT), vacuum infiltration, and a combination of the two proced...

文献信息：Distefano G, La Malfa S, Vitale A, Lorito M, Deng Z, Gentile A.等.  发表于【Transgenic Research】 |  , 请登录

简介：Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents a promising strategy for conferring genetic resistance against a broad range of plant pathogenic fungi. In the present work, two transgenic lemon clones with the chit42 gene from Trichoderma harzianum were tested for resistance to fungal disease and expression level of defence-relat...

文献信息：段艳欣, 范净, 李鼎立, 郭文武.等.  发表于【华中农业大学学报】 |  , 请登录

简介：To screen the transformants rapidly and efficiently and to obtain putative homozygous transgenie plants in a short time as soon as possible，the Agrobacterium tumefaciens mediated transformarion of embryogenic calli from Ponkan(Citrus reticulata Blanco)，Bingtang sweet orange(C sinensis Osbeck)and Murcott(c．reticulata×C sinensis)were conducted．The sensitivities of embryogenic calli and embryos ...

文献信息：Dutt M, Orbovic V, Barthe G, Pasquali G, Gray D, Grosser J, Li Z.等.  发表于【HORTSCIENCE,】 | 请登录

文献信息：Omar A, Calovic M, El-Shamy H, An HJ, Grosser J.等.  发表于【HORTSCIENCE,】 | 请登录

文献信息：A. Ballester, M. Cervera and L. Pe?a等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. The present work investigates three different selection alternatives, comparing them to nptII selection in two citrus genotypes, Carrizo citrange...

文献信息：Zou XP, Li DM, Luo XY, Luo KM, Pei Y.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：Highly efficient Agrobacterium-mediated transformation of trifoliate orange (Poncirus trifoliata (L.) Raf.) was achieved via indirect shoot organogenesis. Stable transformants were obtained from epicotyl segments infected with Agrobacterium strain EHA 105 harboring the binary vector pBI121, which contained the neomycin phosphotransferase gene (NPTII) as a selectable marker and the β-glucuronidase...

文献信息：Orbovic V, Calovic M, G. J.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：Rodríguez A, Cervera M, Peris JE, Peña L等.  发表于【Plant Cell, Tissue and Organ Culture】 | 请登录

简介：In citrus, production of mature transgenic plants belonging to different genotypes is an important biotechnological objective. In the present study, we tried to genetically transform and regenerate mature plants from the economically important Navelina sweet orange cultivar by using the procedure previously established for the genetically close Pineapple sweet orange variety. The use of BAP at 3 m...