文献信息：张亦武等.  请登录

简介：柑橘为世界第一大果树,分布广泛,江苏苏南一带也有种植,而低温胁迫严重制约了柑橘的产量与品质。热激转录因子HSFA2在逆境响应中充当正调控因子,是抗逆基因工程的理想候选基因。但HSFA2基因在柑橘抗寒中的作用尚不明确。因此,本研究拟克隆甜橙HSFA2基因及其启动子,分析其各种胁迫下表达情况,并通过转基因等手段对其抗寒功能进行初步探究。主要结果如下:1、根据柑橘基因组数据库(http://citrus.hzau.cn/orange/),从甜橙中扩增获得1155...

文献信息：Irigoyen, S., Ramasamy, M., Pant, S., Niraula, P., Bedre, R., Gurung, M., Rossi, D., Laughlin, C., Gorman, Z., Achor, D., Levy, A., Kolomiets, M. V., 等.  请登录

简介：A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report...

文献信息：Dutt, Manjul, Mou, Zhonglin, Zhang, Xudong, Tanwir, Sameena E. and Grosser, Jude W.等.  请登录

简介：Background Development of precise genome editing strategies is a prerequisite for producing edited plants that can aid in the study of gene function and help understand the genetic traits in a cultivar. Citrus embryogenic cell cultures can be used to rapidly produce a large population of genome edited transformed citrus lines. The ability to introduce specific mutations in the genome of these cell...

文献信息：Dutt, Manjul, Tanwir, Sameena E., Mahmoud, Lamiaa M., Grosser, Jude W. and Mou, Zhonglin等.  请登录

文献信息：Qiang Li, Anhua Hu, Jingjing Qi, Wanfu Dou, Xiujuan Qin, Xiuping Zou, Lanzhen Xu, Shanchun Chen等.  请登录

文献信息：Qiang Li, Xiujuan Qin, Jingjing Qi, Wanfu Dou, Christophe Dunand, Shanchun Chen, Yongrui He等.  请登录

文献信息：Lugassi, N., Kelly, G., Arad, T., Farkash, C., Yaniv, Y., Yeselson, Y., Schaffer, A. A., Raveh, E., Granot, D. and Carmi, N.等.  请登录

简介：The temporal formation and spatial distribution of stomata on the surface of citrus floral organs and, specifically, on the ovule from which the fruit develops, were analyzed using citrus plants that express green fluorescent protein (GFP) under the guard cell-specific KST1 promoter. Stomata are found on the style, sepal, and anther of the closed flower and on ovules from the stage of anthesis. It...

文献信息：Soares, J. M.; Weber, K. C.; Qiu, W.; Stanton, D.; Mahmoud, L. M.; Wu, H.; Huyck, P.; Zale, J.; Al Jasim, K.; Grosser, J. W.; Dutt, M.等.  请登录

简介：Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo citrange rootstocks expressing either Citrus clementina FT1 or FT3 genes under the control of the Arabidopsis th...

文献信息：Singh, Jagveer; Dhaliwal, Harvinder Singh; Thakur, Anirudh; Sidhu, Gurupkar Singh; Chhuneja, Parveen; Gmitter, Frederick G.等.  请登录

简介：The prevalence of rough lemon rootstock in India is presently threatened by the spread of Phytophthora root rot. Developing an alternative Phytophthora-resistant rootstock that also withstands other abiotic constraints is a priority for a sustainable citrus industry in India. Complementary progenitors can be found in citrus germplasm to combine the desired traits, particularly between Citrus and P...

文献信息：Salonia, Fabrizio; Ciacciulli, Angelo; Poles, Lara; Pappalardo, Helena Domenica; La Malfa, Stefano; Licciardello, Concetta等.  请登录

简介：New plant breeding techniques (NPBTs) aim to overcome traditional breeding limits for fruit tree species, in order to obtain new varieties with improved organoleptic traits and resistance to biotic and abiotic stress, and to maintain fruit quality achieved over centuries by (clonal) selection. Knowledge on the gene(s) controlling a specific trait is essential for the use of NPBTs, such as genome e...

文献信息：Lugassi Nitsan; Kelly Gilor; Arad Tal; Farkash Chagai; Yaniv Yossi; Yeselson Yelena; Schaffer Arthur A; Raveh Eran; Granot David; Carmi Nir等.  请登录

简介：The temporal formation and spatial distribution of stomata on the surface of citrus floral organs and, specifically, on the ovule from which the fruit develops, were analyzed using citrus plants that express green fluorescent protein (GFP) under the guard cell-specific KST1 promoter. Stomata are found on the style, sepal, and anther of the closed flower and on ovules from the stage of anthesis. It...

文献信息：Li, Qiang; Fu, Jia; Qin, Xiujuan; Yang, Wen; Qi, Jingjing; Li, Zhengguo; Chen, Shanchun; He, Yongrui等.  请登录

简介：The present study was designed to serve as a comprehensive analysis of Citrus sinensis (C. sinensis) pectin acetylesterases (CsPAEs), and to assess the roles of these PAEs involved in the development of citrus bacterial canker (CBC) caused by Xanthomonas citri subsp. citri (Xcc) infection. A total of six CsPAEs were identified in the genome of C. sinensis, with these genes being unevenly distribut...

文献信息：Kaur, Kirandeep; Kumar, Krishan; Kaur, Kamaljit; Arora, Parshotam Kumar; Singh, Kuldeep等.  请登录

简介：Kinnow ( Citrus nobilis x C. deliciosa) is a commercially important mandarin but contains large number of seeds. Mukaku Kishu (C. kinokuni), a seedless mandarin can be used to transfer seedlessness in Kinnow through hybridization. But, the polyembryony in Kinnow and absence of any heritable morphological trait in Mukaku Kishu are limiting factors in early identification of hybrids. SSR markers due...

文献信息：Zhang, Shuai; Yin, Zhao-Ping; Wu, Xiao-Meng; Li, Chao-Chao; Xie, Kai-Dong; Deng, Xiu-Xin; Grosser, Jude W.; Guo, Wen-Wu等.  请登录

简介：Cytoplasmic male sterility (CMS) and seedlessness are highly desirable traits for citrus. However, a high-quality reference mitochondrial genome of citrus is unavailable, and the mechanisms of CMS remain to be elucidated in citrus. We previously generated a male sterile somatic cybrid of pummelo (G1+HBP) by fusing protoplasts from CMS 'Guoqing No. 1' Satsuma mandarin (G1) and fertile 'Hirado Bunta...

文献信息：Zheng, Xiongjie; Zhu, Kaijie; Ye, Junli; Price, Elliott J.; Deng, Xiuxin; Fraser, Paul D.等.  请登录

简介：This work reports the development of suspension culture system of transgenic Marsh grapefruit (Citrus paradisi Macf., Rutaceae) callus overexpressing bacterial phytoene synthase; and the use of this suspension culture to investigate the effects of beta-cyclocitral on carotenoid content and composition. At a beta-cyclocitral concentration of 0.5 mM and after ten days cultivation, analysis of the ca...

文献信息：张印; 万勇; 张婷; 叶俊丽; 邓秀新等.  请登录

简介：类胡萝卜素裂解双加氧酶(CCD)可催化类胡萝卜素在特定位点氧化裂解生成多种生物活性化合物,其活性差异一定程度上决定了植物体内类胡萝卜素含量和组成变化。为了探讨柑橘中CCD1蛋白对类胡萝卜素积累的影响,从红肉脐橙(Citrus sinensis L. Osbeck)果实中克隆了CCD1基因,将其特异片段通过重组反应连接到pHGRV载体中,构建CCD1的RNA干扰载体pHGRV-CCD1。利用农杆菌介导的方法将其转入‘国庆1号’蜜柑、‘伏令夏橙’和‘马叙’葡萄柚愈伤组...

文献信息：何珍玉等.  请登录

简介：柑橘深受全世界消费者喜爱,其品质的改良是柑橘育种工作长期核心目标之一。柑橘果实品质与初生和次生代谢物的构成密切相关,品质相关代谢产物合成调控机理的研究成为柑橘前瞻性育种必要的理论基础。转录因子对植物代谢起重要调控作用。本研究筛选到分别响应IAA和冷胁迫的转录因子基因Cs AGL6和Cs PIF8,并对其调控果实色泽、风味及逆境品质相关代谢物质合成的模式进行进一步解析。获得以下研究结果:1.IAA处理对柑橘果实和愈伤组织代谢...

文献信息：黄其椿; 卢东长城; 陈东奎; 江东; 刘吉敏; 谢健; 施平丽; 张兰; 刘福平等.  请登录

简介：采集不同品种的柑橘叶片标样,进行SSR分子标记检测和筛选,确认SSR分子标记准确有效。然后在不同时期、不同地块采集更多品种的叶样,进行SSR分子标记检测,比对试验结果与采样记录,检查SSR分子标记能否准确有效的鉴定沃柑品种。结果表明,3对SSR分子标记可以准确有效地从16个广西、重庆、四川种植的柑橘品种中鉴定出沃柑和无核沃柑(但沃柑和无核沃柑两者之间无法有效区分),该品种鉴定技术可应用于沃柑产业化发展中。

文献信息：解凯东; 方燕妮; 伍小萌; 谢宗周; 邓秀新; 郭文武等.  请登录

简介：‘华柚2号’是将‘国庆1号’温州蜜柑愈伤组织原生质体与‘华柚1号’叶肉原生质体融合创制的二倍体雄性不育胞质杂种。树势中等,花瓣短而退化,雄蕊败育,雌蕊正常。果实扁圆形,隔离种植下完全无核,平均单果质量1 232.47 g,果皮中等厚,可食率57.03%。果肉粉红色,囊壁薄,果肉化渣多汁,风味浓,可溶性固形物12.37%～13.33%,总酸0.90%～1.20%,固酸比10.42～14.13,维生素C24.26 mL·L-1。在湖北武汉地区种植,果实11—12月成熟,5年生嫁接树单...

文献信息：李娜; 邓子牛; 李大志; 张勇; 李树举; 彭际淼; 龙桂友等.  请登录

简介：特早熟柑橘新品种‘橘湘早’由‘大分早生’温州蜜柑中发现的特早熟芽变选育而成。树势强。果实扁圆,果形指数0.78～0.80,平均单果质量115 g;完熟后果皮橙色,光滑,果肉味浓、化渣,含可溶性固形物12.2%,可滴定酸0.55%,维生素C 385 mg·L-1。9月上中旬成熟,采收期长,丰产稳产,平均产量65 280 kg·hm-2。