文献信息：Distefano, G.,Cirilli, M.,Casas, G. L.,La Malfa, S.,Continella, A.,Rugini, E., Thomas, B. , Long, G. , Gentile, A., Muleo, R. 等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Phytochromes are photoreceptors that regulate signal transduction of metabolic pathways and plant development in response to changes in environmental light quality and levels. Studies have shown that mutant plants deficient in phytochromes and transgenic plants overexpressing phytochrome genes exhibit altered canopy growth characteristics. Troyer citrange was transformed with a binary vector syste...

文献信息：Gong, X. Q.,Liu, J. H.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 | 请登录

简介：Citrus is the most important tree fruit crop in the world. However, citrus production is affected by both biotic and abiotic stresses, including drought, extreme temperature, salinity, citrus canker, citrus tristeza virus, and Huanglongbing (or citrus greening), among others. These stresses can severely influence growth and development of both rootstocks and/or scions of citrus trees, thus reducin...

文献信息：Orbovic, V.,Gollner, E. M.,Soria, P.等.  发表于【Acta Physiologiae Plantarum】 |  , 请登录

简介：A possible role of arabinogalactan proteins in control of shoot regeneration from stem explants of two citrus cultivars, Carrizo citrange and 'Duncan' grapefruit, was investigated. Treatment of explants with (beta-d-Glc)(3) Yariv phenylglycoside, able to bind specifically to AGPs, led to a decrease of cumulative regeneration potential of both Carrizo citrange and 'Duncan' grapefruit. For Carrizo, ...

文献信息：Zhu, S. P.，Wu, B.，Ma, Y. Y.，Chen, J.，Zhong, G. Y.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Two trifoliate oranges (Poncirus trifoliata var. monstrosa and P. trifoliata cv \'Xiaoganzhi\') were used separately as pollinators to cross with Shantou-Suanju (Citrus sunki Hort. ex. Tan.) for breeding new citrus rootstocks. Embryos from the seeds of mature fruits were rescued and individually cultured in vitro. On average, 9.0-10.0 embryos per seed were harvested from the two crosses. The zygot...

文献信息：Yildiz, E.，Kaplankiran, M.，Demirkeser, T. H.，Uzun, A.，Toplu, C.等.  发表于【Notulae Botanicae Horti Agrobotanici Cluj-Napoca】 |  , 请登录

简介：Turkey is an important citrus-producing country. However, new cultivars are needed to sustain citrus production and ensure its competitiveness against other crops. There are currently several citrus breeding programs that aim to help overcome the lack of local commercial varieties and to contribute CO Turkey\'s competitive capacity in the citrus market. In this study, we report the utilization of ...

文献信息：Stover, E.Stange, R. R.McCollum, T. G.Jaynes, J.Irey, M.Mirkov, E.等.  发表于【Journal of the American Society for Horticultural Science】 | 请登录

简介：Huanglongbing {HLB [associated with Candidatus Liberibacter sp. (CLas)]} and asiatic citrus canker {ACC [causal organism Xanthomonas citri ssp. citri (XCC)]} are bacterial diseases that seriously threaten sustainability of the Florida citrus (Citrus sp.) industry. Sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) are highly susceptible to ACC and improvement through conventional bree...

文献信息：Jin, S. B,Sun, H. J,Al Bachchu, M. A,Chung, S. J,Lee, J,Han, S. I,Yun, J. H,Boo, K. W,Lee, D.Riu, K. Z.Kim, J. H.等.  发表于【Journal of the Korean Society for Applied Biological Chemistry】 |  , 请登录

简介：Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin L-1. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction mediu...

文献信息：Guo, W. W,Xiao, S. X,Deng, X. X.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Somatic hybridization holds great potential in citrus improvement by circumventing several biological barriers such as nucellar polyembryony, long juvenility and pollen/ovule sterility. To date more than 300 intergeneric and interspecific citrus somatic hybrids have been produced by standard symmetric fusion; among which, more than forty parental combinations produced leaf-parent-type cybrids or p...

文献信息：Felipe, R. T. A,Mourao, Fda,Lopes, S. A,Mendes, B. M. J,Behling, M,Pereira, E. V.等.  发表于【Pesquisa Agropecuaria Brasileira】 |  , 请登录

简介：The objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pera, and Valencia cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB-infect...

文献信息：Attilio, L. B,Mourao, F. D. A,Harakava, R,da Silva, T. L,Miyata, L. Y,Stipp, L. C. L,Mendes, B. M. J.等.  发表于【Pesquisa Agropecuaria Brasileira】 |  , 请登录

简介：The objective of this work was to produce transgenic 'Pera'. and 'Valencia' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co-cultivated with Agrobacterium tumefaciens. Six plants from 'Pera' ...

文献信息：TOUQEER AHMAD等.  请登录

简介：花粉和种子介导的基因漂流现象早已引起科学界的严重关切。为了解决这个问题,科学家利用loxP-FRT融合位点能被FLP重组酶识别的特性,开发了转基因删除技术。该技术能自动、高效地清除转基因烟草花粉和种子中所有的外源功能基因,对营养繁殖的植物,特别是园艺作物具有重要的应用价值。柑橘是一类非常重要的果树,主要通过营养繁殖。由于其童期较长(一般5到12年),严重制约了传统的有性杂交和遗传学研究。不过,类似于草本模式作物,如拟南芥...

文献信息：李孟娇等.   , 请登录

简介：果实特异启动子在利用转基因技术改良果实品质性状中起着至关重要的作用，分离柑橘果实成熟特异启动子并分析其功能对揭示果实成熟的分子机制以及利用生物技术提高果实品质均有十分重要的意义。但是目前关于柑橘果实成熟特异启动子的研究报道较少，本研究在前期获得柑橘果实成熟特异基因的基础上，利用Genome Walking技术分离了该基因的5’上游启动子区域，并通过生物信息学软件分析预测其功能元件；构建了三个不同启动子功能区域驱动...

文献信息：解凯东,王晓培,谢宗周,郭文武等.  发表于【中国南方果树】 |  , 请登录

简介：对已开花结果的4个柑桔体细胞杂种砧木资源,于秋季收集体细胞杂种成熟果实,剥取种子统计单果平均种子数,离体播种观察是否具有多胚特性,以期筛选具有应用潜力的新型体细胞杂种砧木。体细胞杂种哈姆林甜橙+粗柠檬、朋娜脐橙+粗柠檬、朋娜脐橙+枸头橙的单果平均种子数较高,分别为19.9、20.0和19.7粒,且种子饱满、多胚,播种后容易萌发,实生苗生长健壮、整齐一致,从种子特性角度可作为首选砧木应用。Page桔柚+枳的单果平均种子数仅为3.0

文献信息：解凯东,王惠芹,王晓培,梁武军,谢宗周,伊华林,邓秀新,Grosser Jude W,郭文武等.  发表于【中国农业科学】 |  , 请登录

简介：【目的】以单胚性的二倍体柑橘品种为母本与异源四倍体体细胞杂种杂交培育三倍体植株。【方法】选择亲本配置杂交组合进行人工授粉,授粉后70—100 d采幼果进行胚挽救,以流式细胞仪结合根尖染色体压片对再生植株进行倍性检测,最后进行SSR分子标记鉴定。【结果】2009—2012连续4年,以8个二倍体为母本、以4个异源四倍体体细胞杂种为父本,共配置14个倍性杂交组合,授粉花数3 347朵,坐果数678个,平均坐果率20.26%。实施胚挽救的果实数505个...

文献信息：胡威等.  请登录

简介：农杆菌介导的柑橘遗传转化效率一直不高,为进一步提高柑橘的遗传转化效率,本研究以金柑、糖橙及柠檬实生苗上胚轴节间茎段为试材,比较系统地研究影响其再生及转化效率的各种因素,完善它了们的遗传转化技术体系。同时,在优化遗传转化技术体系的基础上,成功将“外源基因清除系统”(Gene-Deletor)以及“果实无核化”基因导入金柑和糖橙橙中,同时将YFP基因导入柠檬中。主要研究结果如下： 1金柑再生及遗传转化技术体系的建立 以35d苗龄...

文献信息：杜发秀等.   , 请登录

简介：类胡萝卜素是一类含有异戊二烯结构单位的化合物的总称，分为胡萝卜素和叶黄素两大类。各种类胡萝卜素都有着其独特的生理功能，对人体的健康有着非常重要的作用。香蕉(MusaSPP.)是继水稻、小麦和玉米之后的世界第四大粮食作物。是我国华南地区农民的主要经济来源，对农村社会和热区经济的发展有着与日俱增的重要性。通过遗传转化提高香蕉中类胡萝卜素的含量，具有重要的理论和实践意义。 本研究主要利用农杆菌介导法，对柑橘八氢番...

文献信息：朱世平,江东,洪棋斌,龚桂芝,刘小丰,赵晓春等.  发表于【中国南方果树】 |  , 请登录

简介：柑桔主要通过嫁接进行繁殖。柑桔砧木对接穗生长势、产量、果实大小、品质和抗性等都有直接的影响。砧木遗传改良是保持柑桔产业具有较强竞争力和较高生产力的重要步骤,世界各国都在致力于柑桔优良砧木的培育。本文就近几十年来柑桔砧木育种进展进行简要综述。

文献信息：刘真真等.   , 请登录

简介：柑橘是我国第二大果树，浙江省是我国柑橘主产区。面对激烈的市场竞争，提高柑橘果实品质，培育优良品种成为发展柑橘产业的关键。目前，关于柑橘果实品质形成的研究主要集中在糖酸代谢、色泽、贮藏保鲜、以及类胡萝卜素代谢等方面，对于果实成熟相关基因的研究报道并不多。研究果实成熟相关基因的表达及调控，对揭示果实成熟的分子机制及利用生物技术来提高果实品质均有十分重要的意义。前期研究获得了一个柑橘果实成熟特异表达基因...

文献信息：姚利晓,何永睿,邹修平,雷天刚,许兰珍,彭爱红,陈善春等.  发表于【果树学报】 |  , 请登录

简介：由于柑橘具有童期长、基因高度杂合、多胚性、远缘杂交不亲和及性器官败育等生物学特性,在很大程度上限制了传统育种的发展,基因工程育种能高效地对植物进行定向改良,将成为柑橘定向改良育种研究工作的重要手段。近十余年来,已成功利用农杆菌侵染法将外源基因转入到越来越多的柑橘品种,并建立柑橘成年态材料的遗传转化体系和标记基因删除系统。对近年来柑橘成年态材料转化、标记基因的更替和转基因柑橘安全性研究等方面的成果进行了总...

文献信息：杨莉,胡威,谢玉明,李义,邓子牛等.  发表于【中国园艺学会2013年学术年会】 |  , 请登录

简介：截至2012年,全球转基因作物种植面积达1.7亿hm~2,转基因作物带来巨大经济和社会效益的同时也引起了人们对转基因食品和环境安全的担忧,阻碍转基因技术的进一步发展。外源基因清除技术‘Gene-deletor'由美国康涅狄格大学李义教授发明,此技术利用器官或组织特异启动子、重组酶及融合识别位点构建基因表达元件,能将转基因植物中的全部外源基因在完成其功能作用后自动地从花粉、种子和果实中彻底清除。在本研究中,将外源基因清除技术应用...