文献信息：Soler N, Plomer M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L. 等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus tristeza virus (CTV), the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins acting at intra- (p23 and p20) and inter-cellular level (p20 and p25) to overcome host antiviral defense. RNA interference (RNAi), an approach based on using dsRNA to trigger RNA silencing, has been widely used for generating transgenic plant resistant agai...

文献信息：Pons E, Alquézar B, Rodríguez A, Martorell P, Genovés S, Llopis S, González N, Ramón D, Rodrigo M.J, Zacarías L, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：β-Carotene is one of the most efficient carotenoid singlet oxygen quenchers and may interfere in free radical oxidation, typical of many degenerative diseases. Besides its activity as protective agent against oxidative stress, βヽarotene also plays a nutritional role as precursor of vitamin A with twice the efficiency of any other pro-vitamin A carotenoid. Orange fruit is an excellent natural di...

文献信息：Rodríguez A, Shimada T, Cervera M, Alquézar B, Gadea J, Gómez-Cadenas A, de Ollas C, Rodrigo M.J, Zacarías L, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Volatile organic compounds, most of them being terpenes, are signals emitted by plants for communication with the environment. Orange fruit accumulates mainly terpenes in mature peel oil glands, D-limonene accounting for approximately 97% of terpene content. We have generated transgenic orange plants carrying a D-limonene synthase gene in antisense (AS) configuration. Transgenic expression caused ...

文献信息：Grosser JW, Gmitter Jr FG, Ling P, Castle WS.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：A comprehensive citrus breeding and genetics program was established at the UF/CREC during the mid-1980’s combining conventional breeding with tools provided by tissue culture, emerging biotechnologies, molecular genetics and genomics. Scion Improvement: The Florida citrus industry is 90% processing sweet oranges. A major goal has been to develop new cultivars for Not From Concentrate juice produ...

文献信息：王军政等.  请登录

简介：柑桔是世界最重要的商品水果之一,在所有水果种类中,其种植面积和产量均居第一位,是第五大国际贸易农产品。在全球138个国家和地区栽培柑桔,是我国南方农村的经济支柱。然而柑桔产业正面临着各种病虫害的威胁,其中,溃疡病和黄龙病是柑桔产业中具有毁灭性危害的细菌病害,往往给柑桔产业造成巨大的经济损失。因此,解决柑桔病害成为柑桔产业可持续发展的关键性因素,但目前世界柑桔生产中溃疡病的危害仍然没有克服,严重影响果实的品质和经...

文献信息：谭彬, 李鼎立, 徐世晓, 郭文武.等.  发表于【果树学报】 | 请登录

简介：【目的】为了快速有效地利用基因工程的方法获得柑橘无核新种质,【方法】以我国特色多核柑橘优良品种锦橙实生苗上胚轴切段为外植体,采用根癌农杆菌介导法进行能导致种子败育基因CG1-400-RNase的转化;为快速有效地筛选出转化子,在实生苗上胚轴切段转化再生过程中,根据不同发育阶段的组织或器官对抗生素的敏感程度不同采用不同的选择压。【结果】结果表明,在抗性芽再生过程中卡那霉素质量浓度设定为50 mg.L-1,获得的362个抗性芽转入卡...

文献信息：李政利, 彭爱红, 邹修平, 何永睿, 姚利晓, 陈善春.等.  发表于【安徽农业科学】 | 请登录

简介：[目的]建立柑橘转基因成分的多重PCR检测体系。[方法]根据GenBank中pBI121质粒序列和柑橘(Citrus.)Actin基因序列,分别设计CaMV35S启动子、NOS启动子、NOS终止子特异引物和Actin基因的特异引物,建立能同时检测出4种序列的多重PCR检测体系,同时通过正交试验确定该体系的最佳引物浓度和比例及PCR反应体系中各因素的浓度及反应程序,并对该方法的灵敏度进行验证。[结果]试验得到的最佳MPCR反应体系为:10×buffer 2.5μl,25 mmol/L MgCl22...

文献信息：李政利等.  请登录

简介：农业生物技术应用国际服务组织(ISAAA)的统计资料显示,截至2011年,种植转基因作物的国家和地区的数量增加至29个,全球转基因作物年种植面积达到1.6亿hm2,比1996年增长了94倍。目前,国内外上市的转基因生物产品也愈来愈多如：转基因大豆、转基因棉花、转基因玉米、转基因油菜和转基因番茄等,由此引发的安全性问题也引起了人们的广泛关注,如何准确检测和识别转基因作物及其产品,也成为人们关注的热点。 柑桔是世界第一大水果,橙汁是世...

文献信息：Wen L, Tan B, Guo WW.等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic plants, so transgene copy number analysis is identified as one most important task after obtaining transgenic plants. In this paper, TaqMan real-time PCR was used to estimate the copy number of exogenous MAC12.2 and NPTII genes in transgenic precocious trifoliate orange (Poncirus trifolia...

文献信息：Soler N, Plomer M, Fagoaga C, Moreno P, Navarro L, Flores R, Pena L.等.  发表于【Plant Biotechnology Journal】 | 请登录

简介：Citrus tristeza virus (CTV), the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins acting at intra- (p23 and p20) and/or intercellular level (p20 and p25) to overcome host antiviral defence. Previously, we showed that Mexican lime transformed with an intron-hairpin construct including part of the gene p23 and the adjacent 3' untranslated ...

文献信息：Pons E, Peris JE, Pena L.等.  发表于【Bmc Biotechnology】 | 请登录

简介：Background: The future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown GM lines. In this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes beta-glucuronidase (uidA) and neomycin phosphotransferase...

文献信息：Mondal SN, Dutt M, Grosser JW, Dewdney MM.等.  发表于【European Journal of Plant Pathology】 | 请登录

简介：Citrus scab, caused by ElsinoA << fawcettii (anamorph Sphaceloma fawcettii), is a common foliar fungal disease affecting many citrus cultivars, including grapefruit. No commercial grapefruit cultivar is resistant to scab, and the disease results in severely blemished fruit which reduces its marketability. Transgenic 'Duncan' grapefruit trees expressing the antimicrobial attE gene were produced via...

文献信息：Khan EU, Fu XZ, Liu JH.等.  发表于【 Plant Cell Tissue and Organ Culture】 | 请登录

简介：In this study, attempts were made to develop a protocol for regeneration of transgenic plants via Agrobacterium tumefaciens-mediated transformation of leaf segments from 'Valencia' sweet orange (Citrus sinensis L. Osbeck) using gfp (green fluorescence protein) as a vital marker. Sensitivity of the leaf segments regeneration to kanamycin was evaluated, which showed that 50 mg l(-1) was the best amo...

文献信息：Favero P, Mourao FDA, Stipp LCL, Mendes BMJ.等.  发表于【Acta Physiologiae Plantarum】 | 请登录

简介：The difficulty in adult tissue genetic transformation in woody species is still an obstacle to be overcome, including in most sweet orange cultivars of the Brazilian citrus industry. This work reports that, after in vitro culture adjustments, transgenic adventitious buds of 'Hamlin', 'Pra', and 'Valencia' sweet oranges (Citrus sinensis L. Osbeck) were recovered using adult material as explant sour...

文献信息：Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.等.  发表于【Tree Physiology】 | 请登录

简介：'Mexican' lime (Citrus aurantifolia Swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolC promoter of Agrobacterium rhizogenes, Arabidopsis thaliana sucrose-H+ symporter (AtSUC2) gene promoter of Arabidopsis thaliana, rice tungro bacilliform virus (RTBV) promoter and sucrose synthase l (RSs1) gene promoter of Oryza sativa (rice). Histoch...

文献信息：Curtis IS, Mirkov TE.等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：The development of a reliable shoot regeneration system for mature tissue of citrus is of major importance to accelerate the evaluation of commercial traits. Three non-ionic surfactants were evaluated independently in terms of their affects on the growth and regeneration of mature internodal stem segments of sweet orange cv. Hamlin in culture. Growth and shoot development of explants were influenc...

文献信息：Cevik B, Lee RF, Niblett CL.等.  发表于【Turkish Journal of Agriculture and Forestry】 | 请登录

简介：Citrus paradisi Macfad. 'Duncan' was transformed with constructs coding for the wild-type and mutant RNA-dependent RNA polymerase (RdRp) of Citrus tristeza virus (CTV) for exploring replicase-mediated pathogen-derived resistance (RM-PDR). The RdRp gene was amplified from a CTV genome and used to generate the wild-type and 2 mutant RdRp constructs for plant transformation. One mutant had the key am...

文献信息：Caruso P, Baldoni E, Mattana M, Paolo DP, Genga A, Coraggio I, Russo G, Picchi V, Recupero GR, Locatelli F.等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Oxygen deficit, which occurs in flooded or poorly drained soils, can limit plant growth and development. Low-oxygen environmental conditions also limit the distribution of many woody plants, such as citrus trees, which are considered flood-sensitive crops, although tolerance to this stress varies among genotypes and rootstocks. In this study, the rice transcription factor Mybleu was inserted into ...

文献信息：Muniz FR, De Souza AJ, Stipp LCL, Schinor E, Freitas W, Harakava R, Stach-Machado DR, Rezende JAM, Mourao FAA, Mendes BMJ.等.  发表于【Biologia Plantarum】 |  , 请登录

简介：Transgenic Citrus sinensis (L.) Osb. plants, cvs. Valencia and Hamlin, expressing Citrus tristeza virus (CTV) derived sequences were obtained by genetic transformation. The gene constructs were pCTV-CP containing the 25 kDa major capsid protein gene (CTV-CP), pCTV-dsCP containing the same CTV-CP gene in an intron-spliced hairpin construct, and pCTV-CS containing a 559 nt conserved region of the CT...

文献信息：MARCOS A. MACHADO , MARI&#194;NGELA CRISTOFANI-YALY , MARINêS BASTIANEL 等.  发表于【Revista Brasileira De Fruticultura】 |  , 请登录

简介：Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus bree...