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  • 681. 柚柠檬苦素葡萄糖基转移酶基因的克隆、表达及植物表达载体的构建

    文献信息:马鑫.等.  请登录

    简介:柠檬苦素类似物是引起柑橘苦味的主要原因之一,柑橘的苦味对柑橘果实的鲜食和加工品质有明显不利影响。此外,柠檬苦素类似物具有抗肿瘤、抗炎、镇痛、昆虫拒食等很多药理活性。由于柠檬苦素类似物具有这些功能,所以柠檬苦素类似物的需求日益增加,但是苦味的存在限制了人们对它的摄取。柠檬苦素类似物配糖体具有水溶性且无苦味等特性,其生物学活性和柠檬苦素类似物是相似的。因而将其作为替代性食品添加剂,有很好的市场前景。 本研究...

    682. 农杆菌介导的MdSPDS1、AhBADH、PtrICE1基因转化柑橘及转基因植株抗性机理研究

    文献信息:付行政等.  请登录

    简介:柑橘是世界最重要的果树之一,其产业的发展对促进地区经济和人类健康具有重要意义。在过去的十年中,柑橘生产取得了巨大进展,但是生物和非生物胁迫一直是制约柑橘生产的重要因素,其中溃疡病、盐害以及低温对柑橘产业造成的经济损失十分惨重。因此培育抗性强的柑橘品种是解决这些问题的关键。由于传统育种手段在柑橘上存在的局限性,利用基因工程技术将一些重要的抗性基因转入柑橘是获得抗性种质最为快捷高效的途径。已有研究表明,多胺...

    683. In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis

    文献信息:Rattanpal HS, Kaur G, Gupta M.等.  发表于【African Journal of Biotechnology】 | 请登录

    简介:This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and hig...

    684. Resistance to Citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-RNA silencing

    文献信息:Reyes CA, Francesco AD, Peña EJ, Costa N, Plata MI, Sendin L, Castagnaro AP, García ML.等.  发表于【Journal of Biotechnology】 |  , 请登录

    简介:The lack of naturally occurring resistance to Citrus psorosis virus (CPsV) has demanded exploitation of a transgenic approach for the development of CPsV-resistant sweet orange plants. Transgenic sweet orange plants producing intron-hairpin RNA transcripts (ihpRNA) corresponding to viral cp, 54K or 24K genes were generated and analyzed at the molecular and phenotypic levels. Two independent CPsV c...

    685. In vitro organogenesis from internodal segments of adult sweet orange plants

    文献信息:Bassan MM, Mourao FDA, Miyata LY, Mendes BMJ.等.  发表于【Pesquisa Agropecuaria Brasileira】 | 请登录

    686. Agrobacterium-mediated transformation using embryogenic calli in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa Wase. Hort

    文献信息:Bachchu MAA, Jin SB, Park JW, Sun HJ, Yun SH, Lee HY, Lee DS, Hong QC, Kim YW, Riu KZ, Kim JH.等.  发表于【Horticulture, Environment, and Biotechnology】 |  , 请登录

    简介:Agrobacterium-mediated transformation in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase was achieved with reasonable transformation efficiency of about 22%, which was the percentage of transgenic plantlets confirmed by genomic PCR (37 plantlets/168 hygromycin-resistant calli). Embryogenic calli of Miyagawa wase were infected with Agrobacterium tumefaciens strain EHA105 harboring binary v...

    687. Functional Expression of Miraculin, a Taste-modifying Protein, in Transgenic Miyagawa Wase Satsuma Mandarin (Citrus unshiu Marc.)

    文献信息:Al Bachchu MA, Jin SB, Park JW, Boo KH, Sun HJ, Kim YW, Lee HY, Riu KZ, Kim JH.等.  发表于【Journal of the Korean Society for Applied Biological Chemistry】 | 请登录

    简介:Miraculin is a taste modifying plant protein, which displays a peculiar property of being able to modify sour taste into sweet taste. Thus, there has been an increasing interest in miraculin due to this amazing property. In the present study, miraculin gene was introduced into the Miyagawa Wase Satsuma mandarin (Citrus unshiu Marc.) callus by Agrobacterum-mediated transformation to produce a citru...

    688. Pollen Competition as a Reproductive Isolation Barrier Represses Transgene Flow between Compatible and Co-Flowering Citrus Genotypes

    文献信息:Pons E, Navarro A, Ollitrault P, Peña L.等.  发表于【PLoS ONE】 |  , 请登录

    简介:Background/Objective: Despite potential benefits granted by genetically modified (GM) fruit trees, their release and commercialization raises concerns about their potential environmental impact, and the transfer via pollen of transgenes to cross-compatible cultivars is deemed to be the greatest source for environmental exposure. Information compiled from field trials on GM trees is essential to pr...

    689. The use of citrus tristeza virus (CTV) containing a green fluorescent protein gene as a tool to evaluate resistance/tolerance of transgenic citrus plants

    文献信息:Orbović V, Soria P, Moore GA, Grosser JW.等.  发表于【Crop Protection】 |  , 请登录

    简介:The T36 strain of citrus tristeza virus (CTV) expressing green fluorescent protein gene (CTVeGFP) was used to detect replication of CTV in wild-type (WT) ‘Hamlin’ sweet orange plants and those transformed with the use of Agrobacterium tumefaciens strain harboring a binary vector with a coat protein gene from either T36 or T30 isolate of CTV. Soil-adapted WT and transgenic plants were challenged ...

    690. Seasonal effects of seed age on regeneration potential and transformation success rate in three citrus cultivars

    文献信息:Orbovic V, Dutt M, Grosser JW.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

    691. Mixture screening and mixture-amount designs to determine plant growth regulator effects on shoot regeneration from grapefruit (Citrus paradisi macf.) epicotyls

    文献信息:Niedz RP, Evens TJ.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 | 请登录

    简介:The objective of this study was to improve shoot regeneration from grapefruit. Because many commercially grown citrus types are apomictic, important in vitro applications such as Agrobacterium-mediated transformation commonly use epicotyl explants from in vitro seedlings; thus, adequate adventitious shoot production is an important prerequisite for efficient use of these applications. Eight plant ...

    692. Genetic transformation efficiency of \'carrizo\' citrange with two gene constructions

    文献信息:Miyata LY, Mourão Filho FAA, Scarpare Filho JA, Zambon F, Bassan MM, Mendes BMJ, Harakava R.等.  发表于【Revista Brasileira de Fruticultura】 |  , 请登录

    简介:Genetic transformation is considered an important tool to be applied in citrus genetic improvement. However, transformation efficiency may vary according to several factors, including the genetic construction utilized. This study was carried out to evaluate the efficiency of genetic transformation of ‘Carrizo’ citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck], containing two...

    693. Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H2O2 production and transcriptional alteration

    文献信息:Fu XZ, Chen CW, Wang Y, Liu JH, Moriguchi T.等.  发表于【BMC Plant Biology】 | 请登录

    简介:Background: Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.
    Results: In the present work, an apple spermidine synthase gene (MdSPDS1) was introduced into sweet orange (Citrus sinensis Osbeck ‘Anliucheng’) via Agr...

    694. Overexpression of the betaine aldehyde dehydrogenase gene from Atriplex hortensis enhances salt tolerance in the transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

    文献信息:Fu XZ, Khan EU, Hu SS, Fan QJ, Liu JH.等.  发表于【Environmental and Experimental Botany】 |  , 请登录

    简介:Trifoliate orange (Poncirus trifoliata L. Raf.), a rootstock widely used for citrus species, is salt-sensitive. Worldwide, salinity is a major abiotic stress affecting citrus growth and yield. Glycinebetaine (GB) is an important osmoprotectant involved in responses to salt stress. However, current evidence regarding the effect of salt stress on GB accumulation in trifoliate orange is limited, and ...

    695. Agrobacterium-mediated transient expression in citrus leaves: a rapid tool for gene expression and functional gene assay

    文献信息:Figueiredo JFL, Römer P, Lahaye T, Graham JH, White FF, Jones JB.等.  发表于【Plant Cell Reports】 |  , 请登录

    简介:In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain Aw in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binary vectors pGWB2 and pGWB5. Infiltration of grapefruit leaves with A. tumefaciens ca...

    696. T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange

    文献信息:Fan J, Liu X, Xu SX, Xu Q, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

    简介:In transgenic plants, multiple T-DNA copies and cytosine methylation usually correlate with a loss of transgene expression while methylation of promoter is associated with transgene silencing. Here, six independent GFP transgenic ‘Bingtang’ sweet orange (Citrus sinensis (L.) Osb.) plants were analyzed with copy number, T-DNA repeat, cytosine methylation and transgene expression. The fluorescence...

    697. Enhanced resistance to canker disease and tolerances to biotic and abiotic stresses in terf1 transgenic sweet orange

    文献信息:Qin L, Luo SL, Deng ZN, Yan JW, Li N, Yuan FR.等.  发表于【Acta Horticulturae】 | 请登录

    698. The sucrose synthase-1 promoter from Citrus sinensis directs expression of the beta-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants

    文献信息:Singer SD, Hily JM, Cox KD.等.  发表于【Planta】 | 请登录

    699. Agrobacterium tumefaciens-mediated transformation of citrus embryonic cells

    文献信息:Dutt M, Grosser JW.等.  发表于【Acta Horticulturae】 | 请登录

    700. Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle)

    文献信息:Dutt M, Vasconcellos M, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

    简介:Four antioxidants including glycine betaine, glutathione, lipoic acid, and polyvinylpyrrolidone were evaluated to improve transformation efficiency of Mexican lime, a precocious but recalcitrant citrus cultivar to Agrobacterium mediated transformation. Lipoic acid substantially improved the transformation efficiency of Mexican lime by aiding in callus development and improving shoot growth from cu...

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