文献信息：Pascale Rossignol , Vladimir Orbovic , Vivian F. Irish 等.  2014-06-09 发表于【Scientia Horticulturae】 |  , 请登录

简介：Inducible gene expression systems, in which the level or timing of activity of a gene of interest can be controlled exogenously, are an effective means to assess gene function. However, such systems have not been widely employed in crop plants. Here we show that the glucocorticoid receptor (GR)-based inducible gene expression system functions in Citrange (Citrus sinensis x C. trifoliata) plants. W...

文献信息：Hongge Jia, Nian Wang*等.  2014-04-07 发表于【Plos One】 |  , 请登录

简介：Genetic modification, including plant breeding, has been widely used to improve crop yield and quality, as well as to increase disease resistance. Targeted genome engineering is expected to contribute significantly to future varietal improvement, and genome editing technologies using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly int...

文献信息：Mohamed, El-Sawy A.Gomaa, Amina,Danial, Nancy等.  发表于【Plant Tissue Culture & Biotechnology】 |  , 请登录

简介：Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT-PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV-free. RAPD analysis was used to asses the genetic ...

文献信息：Thaísa Tessutti Pinheiro, Antonio Figueira and Rodrigo Rocha Latado等.  2014-08-10 发表于【Pinheiro et al. BMC Research Notes】 |  , 请登录

简介：Background: There had been many reports on genetic transformation of Citrus for functional genomic studies but
<br />few included genes associated with flower or fruit traits. A major reason for this might derive from the extensive
<br />juvenile stage of Citrus plants when regenerated from juvenile explants (epicotyls, cotyledon or calli), which delays
<br />the observation of the resulting pheno...

文献信息：Xiuping Zou &#8226; Erling Song &#8226; Aihong Peng &#8226;Yongrui He &#8226; Lanzhen Xu &#8226; Tiangang Lei &#8226;Lixiao Ya等.  2014-01-07 发表于【Plant Cell Tiss Organ Cult】 | 请登录

简介：To improve the resistance of citrus to canker
<br />disease caused by Xanthomonas axonopodis pv. citri (Xac),
<br />it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated
<br />the functionality of PPP1 and hsr203J (Nicotiana tabacum
<br />L.) and gst1 (potato) pathogen-inducible promoters to drive
<br />expression of the b-glucuronidase ...

文献信息：Divanilde Guerra, Maria Teresa Schifino Wittmann, Sergio Francisco Schwarz ,Paulo Vitor Dutra de Souza , Mateus Pereira Gonzatto ,Roberto Luis Weiler 等.  发表于【Bragantia】 |  , 请登录

简介：Tetraploid citrus rootstocks may present different morphological characteristics and growth when compared to diploid ones.
<br />This worked aimed at comparing morphological characteristics and height growth of diploid and tetraploid plants from the
<br />rootstocks ‘Swingle’ citrumelo [C. paradise Macf. x Poncirus trifoliate (L.) Raf], citrange ‘Troyer’ (C. sinensis (L.) Osb. x P. trifoliata)...

文献信息：Shi-Xiao Xu &#8226; Dong-Fang Cai &#8226; Feng-Quan Tan &#8226;Yan-Ni Fang &#8226; Kai-Dong Xie &#8226;, Jude W. Grosser &#822等.  2014-07-01 发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Citrus somatic hybrids produced in the past years provide a novel opportunity to study the immediate effects of allopolyploidization on genome structure and methylation. Here, we present a first attempt to investigate the alterations in genome structure and methylation in three sets of citrus somatic allotetraploids and their diploid parents using amplified fragment length polymorphism (AFLP) and ...

文献信息：Hazarika, T. K.,Hazarika, B. N.,Shukla, A. C.等.  2014-10-31 发表于【Genetic Resources and Crop Evolution】 |  , 请登录

简介：Application of molecular markers, have now been increasingly adopted to address the problems in Citrus taxonomy. Compared to morphological data, molecular tools provide abundant information, highly efficient and are insensitive to environmental factors. Molecular markers has provided an ideal means for identifying genotypes, estimation of relatedness between different accessions and following inhe...

文献信息：Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

简介：Transgenic Citrus sinensis 'Hamlin' and 'Valencia' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detection po...

文献信息：Yahya Abdul-Halim，Chong Gun-Hean，Tan Chin-Ping等.  发表于【Scienceasia】 |  , 请登录

简介：The Merdeka lime is a new citrus hybrid with strong resistance against pests and disease. This work aims to provide information about the total phenolic content (TPC), total flavonoid (TF) content and antioxidant potential of the leaves, peel and pulp of this new hybrid along with those of its parent plants (Citrus hystrix and C. microcarpa). The TPC and TF contents were determined based on a colo...

文献信息：Mahadani, Pradosh,Ghosh, Sankar Kumar等.  2014-07-22 发表于【Molecular Biology Reports】 |  , 请登录

简介：The Consortium of Barcode of Life plant working group proposed to use the defined portion of plastid genes rbcL and matK either singly or in combination as the standard DNA barcode for plants. But DNA barcode based identification of biologically complex plant groups are always a challenging task due to the occurrence of natural hybridization. Here, we examined the use of indels polymorphism in trn...

文献信息：Jamilah Syafawati, Yaacob, Noraini, Mahmad,Rosna Mat, Taha,Normadiha, Mohamed,Anis Idayu, M. Y.,Azani, Saleh等.  发表于【The Scientific World Journal】 |  , 请登录

简介：Various explants (stem, leaf, and root) of Citrus assamensis were cultured on MS media supplemented with various combinations and concentrations (0.5-2.0 mgL -1) of NAA and BAP. Optimum shoot and root regeneration were obtained from stem cultures supplemented with 1.5 mgL -1 NAA and 2.0 mgL -1 BAP, respectively. Explant type affects the success of tissue culture of this species, whereby stem expla...

文献信息：Ramdan, R. Handaji, N. Beyahia, H. Ibriz, M.等.  发表于【Journal of Applied Biosciences】 |  , 请登录

简介：Objective: The aim of this work is to develop a protocol for callus induction of Moroccan Citrus rootstocks. Methodology and results: Callus cultures were initiated from embryos explants of five Citrus rootstocks ( Cleopatra mandarin, Rangpur lime, Citrus volkameriana, Trifoliate orange, Citrus aurantium) on Murashige and Tucker (MT) and Tucker (MT) basal media. Different concentrations of growth ...

文献信息：Jia Hongge,Wang Nian等.  2014-08-22 发表于【Plant Cell Reports】 |  , 请登录

简介：Key message Xanthomonas citri subsp. citri pretreatment before agroinfiltration could significantly promote transient expression in citrus leaves which were previously recalcitrant to agroinfiltration. Transient expression via agroinfiltration is widely used in biotechnology but remains problematic in many economically important plants. Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltra...

文献信息：Cardoso, Jean C.Cardoso, Jean C.Germana, Maria Antonieta,Latado, Rodrigo R.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina x C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. I...

文献信息：Balwinder, Singh等.  发表于【British Biotechnology Journal】 |  , 请登录

简介：The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon ( Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was maximum on MS IV media but maximum cultures...

文献信息：Zou, X. P.，Song, E. L，Peng, A. H.，He, Y. R.，Xu, L. Z.，Lei, T. G.，Yao, L. X.，Chen, S. C.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the beta-glucuronidase (GUS) reporter gene in tran...

文献信息：Xiao, S. X.Biswas, M. K.Li, M. Y.Deng, X. X.Xu, Q.Guo, W. W.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Seedlessness, an important economic trait for fresh fruit, is among the prior goal for all citrus breeding programs. Symmetric somatic hybridization provides a new strategy for citrus seedless breeding by creating cybrids transferring mitochondrial DNA (mtDNA) controlled cytoplasmic male sterility (CMS) from the callus parent Satsuma mandarin (C. unshiu Marc.) to seedy cultivars. In this study, pr...

文献信息：解凯东,王晓培,王惠芹,梁武军,谢宗周,郭大勇,伊华林,邓秀新,Grosser Jude W.,郭文武等.  发表于【园艺学报】 |  , 请登录

简介：2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,共培养幼嫩种子12 578颗,经生芽、生根诱导获得再生植株2 832个;以流式细胞仪对所有再生植株倍性检测,15个组合共获得三倍体401个,四倍体121个;移栽成活三倍体349个,四倍体98个。对W. 默科特 ×NH组合8...

文献信息：邹修平,彭爱红,刘琦琦,何永睿,王军政,许兰珍,雷天刚,姚利晓,陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为了利用CecropinB(CB)抗菌肽基因提高柑橘对溃疡病的抗性,人工合成了两个含有信号肽的Cecropin B抗菌肽基因PR1aCB和AATCB。洋葱表皮瞬时表达分析表明,与非分泌型CB抗菌肽相比,PR1aCB和AATCB抗菌肽在细胞间隙中优势积累。进一步构建了CaMV 35S调控 PR1aCB和AATCB基因的植物表达载体,转化塔罗科血橙(Citrus sinensis Osbeck)上胚轴,获得转基因植株。GUS组织化学染色、PCR和Southern blot分析表明外源基因已成功整合入柑橘基因组。Rea...