文献信息：王淑明,曹洪波,王晓培,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：纯系材料在植物育种及基因组学研究中发挥着重要的作用,尤其在全基因组测序中有着明显的优越性,同时纯系材料还可用于物理图谱基因图谱分析、遗传转化等研究。柑橘类植物具有高度杂合、童期长、多胚性、树体高大等特性,很难通过常规育种获得纯系材料。花药培养是获得柑橘纯系材料快速、有效的途径。本研究以4种不同培养基,分别对红夏橙、早金甜橙、红橘、鄂柑1号椪柑、华农本地早橘、华农红抽、HB抽等7个品种的处于单核靠边期的花药进...

文献信息：曹洪波等.  请登录

简介：类胡萝卜素是植物生存必需的次生代谢类物质,参与众多的生物学过程,如光合作用、抗氧化保护、激素合成以及传粉等。类胡萝卜素也是人类健康所必需的生物活性成份,如提供维生素A前体,抗氧化和衰老,降低心血管疾病风险,以及参与人体免疫、胚胎发育和生殖等。植物是自然界中类胡萝卜素合成的主要生物体,也是人体中类胡萝卜素的最主要来源。因此,研究植物类胡萝卜素代谢调控机制是有效利用类胡萝卜素的重要基础。 柑橘中含有丰富多样的类...

文献信息：肖诗鑫,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：我国作为柑橘起源地,地方良种多但多数是有籽品种,影响了其果实品质和商品价值。因此,实现我国地方特色品种的无核化具有重要的理论和实际意义。温州蜜柑(Citrus unshiu Marc)为细胞质雄性不育(cytoplasmic male sterility,CMS)类型,本研究旨在针对性地开展温州蜜柑与其它有籽柑橘品种的对称融合研究,实现温州蜜柑细胞质雄性不育性状的有效转移。将国庆1号温州蜜柑愈伤组织悬浮系原生质体,分别与桃叶橙、早金甜橙等多籽甜橙的叶肉原生...

文献信息：Marutani-Hert M, Bowman KD, McCollum GT, Mirkov TE, Evens TJ, Niedz RP.等.  发表于【Plos One】 |  , 请登录

简介：Background: Citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production ...

文献信息：Sendin LN, Filippone MP, Orce IG, Rigano L, Enrique R, Pena L, Vojnov AA, Marano MR, Castagnaro AP.等.  发表于【Plant Pathology】 | 请登录

简介：susceptible host is diminished, indicating that the avrBs2 gene product could confer an adaptive advantage to the pathogen. The avrBs2 of Xanthomonas citri subsp. citri (Xcc), cause of citrus canker, shares 96% homology with avrBs2 of Xcv. To evaluate if Bs2 could recognize avrBs2 of Xcc in citrus plants and thereby activate plant defence mechanisms to increase resistance to canker, transient expr...

文献信息：Dutt M, Barthe G.A, Orbovic V, Irey M, Grosser J.W等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Transgenic citrus scion and rootstock cultivars were transformed with gene(s) encoding antimicrobial peptides or systemic acquired resistance (SAR) proteins. Each gene was under control of an enhanced CaMV 35S promoter. Several genes were also under control of a phloem specific Arabidopsis SUC2 (AtSUC2) promoter. A number of clones of each transgenic line were evaluated for resistance to Huanglong...

文献信息：Oliveri C, Distefano G, La Malfa S, La Rosa R, Deng Z, Gentile A.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents an efficient strategy to confer genetic resistance against a broad range of phytopathogenic fungi. In lemon (Citrus limon), fungal diseases cause economic losses in the field as well as in postharvest management. In this study, fruits collected from two different transgenic lemon cl...

文献信息：Castro LM, Mendes BMJ, Mourão Filho FAA等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：The use of mature tissue for citrus genetic transformation is important for the precocious evaluation of the genetically modified characteristics. However, an efficient organogenesis protocol with high rates of plant regeneration is necessary. This work aimed to optimize a protocol for in vitro organogenesis of ‘Hamlin’, ‘Pera’, and ‘Valencia’ sweet orange cultivars (Citrus sinensis) using a...

文献信息：Tallón CI, Porras I, Pérez-Tornero O.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：The influence of the basal medium and different plant growth regulators on the efficient micropropagation of nodal explants from mature trees of sour orange and ‘Cleopatra’ mandarin citrus rootstocks was studied. The preference of all two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed. Several combinations of BA and AD, KIN or GA were made in an attempt to opti...

文献信息：Xie YM, Yang L, Hu W, Li Y, Deng ZN.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：High level of contamination, low morphogenetic potential and recalcitrance to Agrobacterium tumefaciens infection are the main problems in the transformation of adult citrus tissues. A series of experiments was performed to solve these problems. ‘Newhall’, ‘Succari’ and ‘Bingtang’ sweet orange cultivars were tested. Adult shoots from the 3 cultivars were grafted and re-grafted on Poncirus tr...

文献信息：Yang L, Hu W, Xie YM, Li Y, Deng ZN.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Internodal stem segments from citrus seedlings have been widely used as explants in transformation research, due to their ability of easy regeneration. Since 1990, genetic transformation has been applied on different citrus species including sweet oranges (Citrus sinensis), mandarins (C. reticulata), trifoliate orange (Poncirus trifoliata), sour orange (C. aurantium), pummelo (C. grandis), citrang...

文献信息：Jensen SP, Febres VJ, Moore GA.等.  请登录

简介：Citrus transformation is typically Agrobacterium tumefaciens-mediated, in which citrus tissues are cultivated with the bacterium and regenerated on selection media. Part of the bacterial plasmid, with the gene of interest and resistance genes, is then inserted into the citrus genome. Due to the slow growth of citrus, this long process must be optimized for each cultivar and ultimately the transfor...

文献信息：Pons E, Navarro A, Ollitrault P, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Despite potential benefits granted by genetically modified (GM) citrus trees, their release and commercialization raises concerns about their potential environmental impact. The transfer via pollen of transgenes to crosscompatible cultivars is deemed to be the greatest source for environmental exposure. In this work, three different citrus genotypes carrying the uidA (GUS) tracer marker gene (poll...

文献信息：Zou XP, Peng AH, Liu XF, He YR, Xu LZ, Chen SC.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Selectable marker genes conferring antibiotic- or herbicide- resistance are in general required to efficiently recover transgenic plants from transformed cells during plant transformation. However, the presence of marker genes raises public concerns regarding the field release of transgenic plants. Removal of marker genes prevents the risk of its release into the environment and hastens the public...

文献信息：Soler N, Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña L. 等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus tristeza virus (CTV) is phloem-restricted in natural citrus hosts. The 23 kDa protein (p23) encoded by the virus is a RNA silencing suppressor and an important pathogenicity factor. Constitutive expression of p23 in transgenic citrus plants under the control of the 35S promoter incites, in addition to aberrations resembling CTV symptoms, others likely related to the ectopic expression of th...

文献信息：Marques VV, Miller AM, Bagio TZ, Souza GV, Meneguin L, Vieira LGE, Pereira LFP, Leite JRRP.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus Canker caused by Xanthomonas citri subsp. citri (Xcc) is an important disease for citrus production around the world. Several highly valuable commercial citrus cultivars are moderately to highly susceptible to this disease. Development of transgenic plants for disease resistance in economically important citrus cultivars may represent a unique and efficient strategy for control of Citrus Ca...

文献信息：Peris JE, Vicent A, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Phytophthora spp. causes foot rot and gummosis, the most serious soilborne diseases of citrus worldwide. Sweet orange (Citrus sinensis) might be an excellent rootstock but it cannot be used as such in many citrus areas due to its high sensitivity to Phytophthora spp. The development of sweet orange that possess durable genetic resistance provides the best prospect for full recovery of this citrus ...

文献信息：Jin SB, Sun HJ, Kim YW, Bachchu MAA, Yun SH, Riu KZ, Kim JH.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：An efficient system for the production of a recombinant miraculin protein in transgenic citrus plant cells was developed. Miraculin is a taste-modifying protein that can transform a sour taste into a sweet taste. A miraculin gene containing a N-terminal signal peptide was introduced into navel orange (Citrus sinensis) callus cells by Agrobacterium-mediated transformation. Transgenic green color so...

文献信息：Muniz FR, Harakava R, Rezende JAM, Moore GA, Febres VJ, Mourao Filho FAA, Mendes BMJ.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus tristeza virus (CTV) control relies mainly on the use of tolerant rootstocks and scion cross protection. The aim of this work was to evaluate transgenic C. sinensis lines for resistance to CTV, in order to offer another alternative for virus control. Transgenic lines of C. sinensis cv. ‘Valencia’ and ‘Hamlin’ containing one of 3 different genetic constructs (pCTV-CP: coat protein gene o...

文献信息：Kava-Cordeiro V, Rodríguez A, Vicent A, Glienke C, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus Black Spot (CBS) is a disease affecting sweet orange, lemons and mandarin varieties in Africa, Oceania and Americas. In Europe, CBS is a quarantine disease, limiting the import of fresh fruits from citrus-producing countries of those continents. CBS is caused by the fungus Phyllosticta citricarpa, which induces damages in fruit flavedo affecting their appearance and additionally causing pre...