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  • 301. Sweet orange genetic transformation with the attacin A gene under the control of phloem-specific promoters and inoculation with Candidatus Liberibacter asiaticus

    文献信息:da Rocha Tavano Eveline Carla, Erpen Ligia, Aluisi Bianca, Harakava Ricardo, Spotti Lopes Joao Roberto, Carneiro Vieira Maria Lucia, De Stefano Piedad等.  发表于【Journal of Horticultural Science & Biotechnology】 | 请登录

    简介:An alternative approach to control Huanglongbing disease in Citrus is the development of transgenic plants expressing genes that may influence pathogen development. We report herein the production of sweet orange transgenic plants bearing gene constructs containing the antimicrobial gene attacin A (attA) under the control of phloem-specific promoters. Transcripts of the attA gene were accumulated ...

    302. Triploid frequency of sexual hybridization and pollen and ovary development in mandarins

    文献信息:Costa S N, Cortez P A, da Hora Almeida L A, Martins F M, Soares Filho W d S, Coelho Filho M A, Gesteira A d S等.  发表于【 Brazilian Journal of Botany】 | 请登录

    简介:This work aimed to ascertain the triploid frequency of sexual hybridization and the pollen and ovary development in Citrus. The Fortune' mandarin (Citrus clementina Hort. ex Tan.xC. tangerina Hort. ex Tan.) accession was selected as female parent for this study based on its reproductive characteristics, mainly the monoembryony and self-incompatibility. The male parents used were the Cravo' (C. ret...

    303. Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in An Air-Lift Bioreactor Culture

    文献信息:Agisimanto Dita, Normah M. N., Ibrahim Rusli等.  发表于【Agrivita】 | 请登录

    简介:Embryogenic cell (EC) growth and proliferation as well as somatic embryo induction were examined in a bioreactor culture using Murashige and Skoog basal medium particularly for the influence of 6-benzyladenine (BA) concentration, initial cell density and aeration rate. Embryogenic callus was induced from seeds of Citrus reticulata Blanco cv. Madu. The cell suspension in a 3-L bioreactor exhibited ...

    304. Gene Expression in Citrus Plant Cells Using Helios Gene Gun System for Particle Bombardment

    文献信息: Acanda Yosvanis, Wang Chunxia, Levy Amit等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

    简介:To understand how Citrus tristeza virus (CTV) replicates and moves inside the plant, it is critical to study the cellular interactions and localization of its encoded proteins. However, due to technical limitations, so far these studies have been limited to the nonnatural host Nicotiana benthamiana.Particle bombardment is a physical method to deliver nucleic acid and other biomolecules into the ce...

    305. Citrus rootstock breeding: response of four allotetraploid somatic hybrids to Citrus tristeza virus induced infections

    文献信息:Abbate L, Panno S, Mercati F, Davino S, Del Bosco S F等.  发表于【 European Journal of Plant Pathology】 | 请登录

    简介:Four allotetraploid somatic hybrids of citrus, with potential for rootstock improvement, have been evaluated for their response to Citrus tristeza virus (CTV) infection. CTV is the most important viral pathogen affecting citrus production worldwide. Somatic combinations of Milam' lemon (Citrus jambhiri Lush.)+Sour orange (C. aurantium L Osb.), Calamondin (C. madurensis Lour.)+Keen' sour orange (C....

    306. Citrus Breeding 2.0: A Novel Approach Integrating Deciphered Parentage and Genomics-assisted Selection

    文献信息:Shimizu Tokurou等.  发表于【Jarq-Japan Agricultural Research Quarterly201】 | 请登录

    简介:Current citrus breeding programs have three objectives: developing diverse varieties that satisfy assorted commercial needs; improving overall fruit quality; and releasing varieties quickly. However, these objectives may conflict with each other owing to the limited available orchard space, and conventional breeding systems have not progressed rapidly enough to resolve this problem. "Citrus Breedi...

    307. The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker

    文献信息:Sendin Lorena Noelia, Filippone Maria Paula等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

    简介:Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to t...

    308. Improved protocol for the transformation of adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues

    文献信息:Peng A, Zou X, Xu L, He Y, Lei T, Yao L, Li Q, Chen S等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 | 请登录

    简介:The production of transgenic citrus plants from adult tissues is difficult because of low regeneration and transformation rates. To increase the transformation efficiency of adult citrus tissues, an improved protocol involving adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues was developed. Explants were pre-incubated in a liquid medium prior to infection by Agrobacterium tumefaciens. ...

    309. Novel Plastid-Nuclear Genome Combinations Enhance Resistance to Cirtrus Canker in Cybrid Grapefruit

    文献信息:Murata M M, Omar A A, Mou Z, Chase C D, Grosser J W, Graham J H等.  2019-01-07 发表于【Frontiers in Plant Science】 | 请登录

    简介:Host disease resistance is the most desirable strategy for control of cirtus canker, a disease caused by a gram-negative bacterium Xanthomonas cirti subsp. citri. However, no resistant commerical citrus has been identified. Cybribization, a somatic hybribization approach that combines the organelle and nuclear genomes from different species, was used to create cybrids between citrus canker resista...

    310. Functional study of Csrbohs in defence response against Xanthomonas citri ssp. citri

    文献信息:Mei P, Song Z, Li Z a, Zhou C等.  请登录

    简介:NADPH oxidases, encoded by rbohs (respiratory burst oxidase homologues), transfer electrons from NADPH to molecular oxygen (O-2) to generate superoxide anion (O-2(center dot-)), which is the first step in the formation of hydrogen peroxide (H2O2) in the plant-pathogen interaction system. In the present work, six citrus rbohs (Csrbohs) genes were identified in citrus, and their possible involvement...

    311. Transcriptomic changes triggered by carotenoid biosynthesis inhibitors and role of Citrus sinensis phosphate transporter 4;2 (CsPHT4;2) in enhancing carotenoid accumulation

    文献信息:Lu Pengjun, Wang Shasha, Grierson Don, Xu Changjie等.  2019-01-01 发表于【Planta】 | 请登录

    简介: Main conclusion Carotenoid accumulation and chromoplast development in orange were perturbed by carotenoid inhibitors, and candidate genes were identified via transcriptomic analysis. The role of CsPHT4;2 in enhancing carotenoid accumulation was revealed. Carotenoids are important plant pigments and their accumulation can be affected by biosynthesis inhibitors, but the genes involved were largely...

    312. Two Genes (ClS1 and ClF-box) Involved the Self-Incompatibility of "Xiangshui" Lemon (Citrus limon (L.) Burm. f.)

    文献信息:Lin W, Zhang S, Ding F, He X, Luo C, Huang G, Do M, Wang Q, Yang Z, Su L, Hu G等.  2019-04-01 发表于【 Plant Molecular Biology Reporter】 | 请登录

    简介:Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in Xiangshui lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the Xiangshui lemon, designated as ...

    313. Potential use of the DREB/ERF, MYB, NAC and WRKY transcription factors to improve abiotic and biotic stress in transgenic plants

    文献信息:Erpen, Ligia, Devi, H. Sunitibala, Grosser, Jude W. and Dutt, Manjul等.  请登录

    简介:Abiotic and biotic stresses adversely affect the growth and productivity of plants and constitute the major yield-limiting factor in agriculture. In recent years, several transcription factors (TF) have been identified that can function as switches to control the expression of potential stress-related genes. These transcription factors play crucial roles in governing transcriptional reprogramming ...

    314. Localized expression of antimicrobial proteins mitigates huanglongbing symptoms in Mexican lime

    文献信息:Guerra-Lupian, M. A., Ruiz-Medrano, R., Ramirez-Pool, J. A., Ramirez-Ortega, F. A., Lopez-Buenfil, J. A., Loeza-Kuk, E., Morales-Galvan, O., Chavarin-等.  请登录

    简介: Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and beta-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Chris...

    315. Metabolically engineered anthocyanin-producing lime provides additional nutritional value and antioxidant potential to juice

    文献信息:Hijaz, Faraj, Nehela, Yasser, Jones, Shelley E., Dutt, Manjul, Grosser, Jude W., Manthey, John A. and Killiny, Nabil等.  请登录

    简介:Anthocyanins are synthesized via the flavonoid pathway through a complex expression of several genes such as MYB transcription factors. Anthocyanins protect plants against biotic and abiotic stresses. Herein, we studied the effect of expression of MYB (VvmybA1 cloned from the red grape and Ruby cloned from Moro' blood orange) transcription factors in Mexican lime on juice quality and leaf pigments...

    316. Localized expression of antimicrobial proteins mitigates huanglongbing symptoms in Mexican lime

    文献信息:Miguel-Angel, Guerra-Lupián, Roberto, Ruiz Medrano, José-Abrahán, Ramírez-Pool, Francisco-Arturo, Ramírez-Ortega, José-Abel, López-Buenfil, Emiliano, 等.  请登录

    简介:Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and β-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Christm....

    317. Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata plus Poncirus trifoliata) Revealed by Molecular Marker Inheritance

    文献信息: Kamiri Mourad, Stift Marc, Costantino Gilles, Dambier Dominique, Kabbage Tariq, Ollitrault Patrick, Froelicher Yann等.  2018-11-02 发表于【Frontiers in Plant Science】 | 请登录

    简介:The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop k...

    318. Citrus sinensis MYB transcription factors CsMYB330 and CsMYB308 regulate fruit juice sac lignification through fine-tuning expression of the Cs4CL1 gene

    文献信息:Jia Ning, Liu Jiqin, Sun Yufeng, Tan Penghui, Cao Hao, Xie Yingying, Wen Boting, Gu Tianyi, Liu Jiameng, Li Minmin, Huang Yatao, Lu Jia, Jin Nuo, Sun 等.  发表于【Plant Science】 | 请登录

    简介:Lignin is one of the most important components of the plant cell wall, and the expression and transcriptional regulation of lignin biosynthesis-related genes have been studied widely in Arabidopsis and other plants. Citrus fruit juice sacs often undergo lignification, particularly during fruit ripening and storage periods; however, the underlying genetic mechanisms have been little investigated. I...

    319. 柑橘原生质体瞬时转化体系优化及利用CRISPR/Cas9技术定点突变山金柑基因

    文献信息:徐旋等.  请登录

    简介:柑橘是世界产量第一的水果,由于童期长、种子多胚、某些重要的商业品种育性低甚至不育等特点,利用传统的育种方法改良柑橘进展缓慢。CRISPR/Cas9系统作为近几年快速发展的基因组编辑技术可以精确有效地诱导特定的突变,特异地改造基因组,已成为植物基因功能研究和作物遗传改良的重要手段之一。利用植物原生质体瞬时转化CRISPR/Cas9系统突变得到的植株,属于非转基因种质资源,能够省去漫长的后代筛选步骤;同时相对于农杆菌介导转化柑橘上...

    320. 柑橘MIR156和MIR172基因家族成员在成花调控中的功能验证

    文献信息:王晨阳等.  请登录

    简介:microRNA(miRNA)是一类由内源基因编码的长度为21-23nt的非编码单链小RNA分子,在调控植物发育方面发挥着重要的作用。柑橘是世界第一大果树,其童期长、结果晚的特性严重影响了其经济效益和育种进程。已有大量研究报道表明,microRNA 156(miR156)和microRNA 172(miR172)参与调控植物成花转变。本研究前期通过转录组测序,共在枳中获得miR156家族3个成员,分别是PtmiR156a,PtmiR156b,PtmiR156c;以及miR172家族4个成员,分别是PtmiR172-03,P...

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