文献信息：Zhou Junhui, Li Dongdong, Wang Guoming, Wang Fuxi, Kunjal Merixia, Joldersma Dirk, Liu Zhongchi等.  发表于【Journal of Integrative Plant Biology】 | 请登录

简介：Fruit crops, including apple, orange, grape, banana, strawberry, watermelon, kiwifruit and tomato, not only provide essential nutrients for human life but also contribute to the major agricultural output and economic growth of many countries and regions in the world. Recent advancements in genome editing provides an unprecedented opportunity for the genetic improvement of these agronomically impor...

文献信息：谢幸男,杨莉,刘范,田娜,车婧如,靳三鹏,张永艳,程春振等.  发表于【园艺学报】 | 请登录

简介：以‘伏令夏橙’实生苗为材料进行原位转化体系建立及优化。首先,研究侵染部位对原位转化效率的影响:使用带有PBI121载体的农杆菌分别侵染5周龄‘伏令夏橙’实生苗上胚轴切口、顶芽切口(保留/去除真叶)2次,共培养3d后进行抗性筛选及暗处理,7周后提取叶片DNA用于转基因植株PCR鉴定;之后,以上胚轴切口作为侵染部位进一步研究苗龄对原位转化效率的影响。研究结果表明,5周龄上胚轴切口再生率为93.10%,转化率为20.68%;保留/去除真叶后侵染顶...

文献信息：孙立方,柯甫志,聂振朋,王平,孙建华,徐建国等.  发表于【浙江农业科学】 | 请登录

简介：柑橘病害严重影响其外观、品质和产量,给生产带来损失。本研究利用LP4/2A作为连接肽,构建AtNPR1和抗菌肽Cecropin B基因的共表达载体,为后期转化柑橘获得抗病材料做准备。以实验室前期构建的质粒为模板扩增AtNPR1基因,并人工合成连接肽LP4/2A和抗菌肽Cecropin B基因的编码序列。利用重叠PCR将AtNPR1和LP4/2A-Cecropin B重组,并连接至表达载体pBI121,获得以35S为启动子的35S:AtNPR1-LP4/2A-Cecropin B的共表达载体。同时,本研究还进一...

文献信息：戴文珊,王敏,刘继红等.  发表于【园艺学报】 | 请登录

简介：为了探明印度酸橘(Citrus reshni)脱落酸合成关键基因CrNCED1的功能,分析了CrNCED1的表达特性,并将其在柠檬中超表达获得转基因植株,对转基因植株进行脱水抗性分析。结果表明,CrNCED1在印度酸橘叶片中表达量最高;并且其表达受脱水、低温和ABA诱导,但不受盐诱导,其中ABA诱导上调作用强烈。超表达CrNCED1的转基因柠檬植株ABA含量显著高于野生型,脱水处理后叶片萎蔫程度比野生型轻,其相对失水率、MDA和活性氧含量均显著低于野生型,叶片气...

文献信息：Aljasim, Kawther, Dutt, Manjul, Grosser, Jude W. and Soares, Juliana M.等.  请登录

文献信息：Calovic, Milica, Chen, Chunxian, Yu, Qibin, Orbovic, Vladimir, Gmitter, Frederick G., Jr. and Grosser, Jude W.等.  请登录

简介：Six mandarin cultivars, Ponkan (Citrus reticulata), Willowleaf (Citrus deliciosa), Kinnow (Citrus nobilis x C. deliciosa), Murcott (purported C. reticulata x Citrus sinensis), W. Murcott [purported (C. reticulata x C. sinensis) x C. reticulata)], and Snack (purported C. reticulata hybrid), were used in protoplast fusion with different parental combinations to generate somatic hybrids. Sixty-five s...

文献信息：Qiang Li, Anhua Hu, Wanfu Dou, Jingjing Qi, Qin Long, Xiuping Zou, Tiangang Lei, Lixiao Yao, Yongrui He, Shanchun Chen等.  请登录

文献信息：Qiang Li, Ruirui Jia, Wanfu Dou, Jingjing Qi, Xiujuan Qin, Yongyao Fu, Yongrui He*, Shanchun Chen等.  请登录

文献信息：Sandhu, J. S., Nayyar, S., Kaur, A., Kaur, R., Kalia, A., Arora, A., Kaur, Y., Thind, S. K. and Chhabra, G.等.  2019-05-16 请登录

文献信息：Soares, Juliana M., Qiu, Wenming, Grosser, Jude W. and Dutt, Manjul等.  请登录

文献信息：Zou Xiuping, Long Junhong, Zhao Ke, Peng Aihong, Chen Min, Long Qin, He Yongrui, Chen Shanchun等.  2019-12-12 发表于【Plos One】 | 请登录

简介：The auxin early response gene Gretchen Hagen3 (GH3) plays dual roles in plant development and responses to biotic or abiotic stress. It functions in regulating hormone homeostasis through the conjugation of free auxin to amino acids. In citrus, GH3.1 and GH3.1L play important roles in responding to Xanthomonas citri subsp. citri (Xcc). Here, in Wanjingcheng orange (Citrus sinensis Osbeck), the ove...

文献信息：Shi Cai-Yun, Hussain Syed Bilal, Yang Huan, Bai Ying-Xin, Khan Muhammad Abbas ,Liu Yong-Zhong等.  发表于【Plant Science,<Go to ISI>://WOS:000494891900026】 | 请登录

简介：Citric acid homeostasis patterns and its content are diversified among the fruits of citrus cultivars, but the cause remained unclear. In this study we showed that changes of citric acid content were highly associated with the expression profiles of a P-type proton pump gene (CsPH8) in the fruits of six citrus cultivars; moreover, analysis of 21 different fruit samples indicated that the correlati...

文献信息：Liu Xiao, Guo Ling-Xia, Luo Li-Juan, Liu Yong-Zhong, Peng Shu-Ang等.  发表于【Plant Molecular Biology】 | 请登录

简介：Key message: At least eight MGT genes were identified in citrus and PtrMGT5 plays important role in maintaining Mg homeostasis in citrus by getting involved in the Mg absorption and transport. Abstract: Magnesium (Mg) is an essential macronutrient for plant growth and development, and the magnesium transporter (MGT) genes participate in mediate Mg2+ uptake, translocation and sequestration into cel...

文献信息：Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2020-07-19 发表于【Tree physiology】 | 请登录

简介：Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants ...

文献信息：Hasan Nazmul, Kamruzzaman Mohammad, Islam Shariful, Hoque Hammadul, Bhuiyan Fahmid Hossain, Prodhan Shamsul H.等.  2020-10-16 发表于【Journal of Genetic Engineering and Biotechnology】 | 请登录

简介：Background Recent studies indicate that farmers are facing several challenges due to biotic and abiotic stresses like diseases, drought, cold, and soil salinity which are causing declined Citrus production. Thus, it is essential to improve these varieties which would be resistant against biotic and abiotic stresses as well as high yielding. The transformation of abiotic stress tolerant genes in Ci...

文献信息：Huang Ding, Yuan Yue, Tang Zhouzhou, Huang Yue, Kang Chunying, Deng Xiuxin, Xu Qiang等.  发表于【Plant Cell and Environment】 | 请登录

简介：Blood orange is generally recognized to accumulate anthocyanins in its fruit pulp in a cold-inducible manner. We observed that the fruit peel of blood orange can also accumulate anthocyanins under ample light conditions. Interestingly, purple pummelo can accumulate anthocyanins only in its fruit peel but not in its pulp. The mechanism underlying the tissue specificity of anthocyanin accumulation i...

文献信息：Killiny Nabil, Nehela Yasser等.  发表于【Plant Cell Reports】 | 请登录

简介：Key message: In citrus, abscisic acid-deficiency was associated with a dwarfing phenotype, slow growth, small leaves, decreased fresh weight, and faster water loss. ABA supplementation reversed the dwarfing phenotype and enhanced growth.AbstractAbscisic acid (ABA) is a ubiquitously distributed phytohormone, which is almost produced by all living kingdoms. In plants, ABA plays pleiotropic physiolog...

文献信息：Xie Kai-Dong ,Yuan Dong-Ya, Wang Wei, Xia Qiang-Ming, Wu Xiao-Meng ,Chen Chuan-Wu, Chen Chun-Li ,Grosser Jude W, Guo Wen-Wu等.  2019-06-27 发表于【Scientia Horticulturae】 | 请登录

简介：Seedlessness is a primary breeding objective for citrus fresh fruit market, and triploids have been proven to have great value to produce seedless fruits. In this study, aiming to produce triploid plants for developing some seedless cultivars, four 2x x 4x interploid crosses were conducted using three elite but seedy cultivars as seed parents and one newly flowered doubled diploid and two allotetr...

文献信息：Xiongjie Zheng,Kaijie Zhu,Quan Sun,Weiyi Zhang,Xia Wang,Hongbo Cao,Meilian Tan,Zongzhou Xie,Yunliu Zeng,Junli Ye,Lijun Chai,Qiang Xu,Zhiyong Pan,Shuny等.  请登录

简介：Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference. Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments. In this study, using an integrated genetic approach we revealed that a 5' cis-regulatory change at CCD4b encoding CAROTE...

文献信息：邹修平,龙俊宏,彭爱红,陈敏,龙琴,陈善春等.  2019-11-01 发表于【中国农业科学】 | 请登录

简介：背景: 由柑橘黄单胞杆菌柑橘亚种（Xanthomonas citri subsp. citri）引起的柑橘溃疡病是柑橘生产上最具毁灭性的一种病害。植物生长素在调控柑橘溃疡病菌引起的寄主侵染部位脓疱形成中起重要作用。生长素早期响应基因GH3通过酰基化吲哚-3-乙酸（indole-3-acetic acid, IAA）调控植物激素动态平衡。前期研究发现柑橘CsGH3.6在调控生长素响应溃疡病侵染中起着重要作用。目的: 通过对超量表达CsGH3.6转基因晚锦橙的抗病性、植株表型、细...