文献信息：Lin W, Zhang S, Ding F, He X, Luo C, Huang G, Do M, Wang Q, Yang Z, Su L, Hu G等.  2019-04-01 发表于【 Plant Molecular Biology Reporter】 | 请登录

简介：Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in Xiangshui lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the Xiangshui lemon, designated as ...

文献信息：Erpen, Ligia, Devi, H. Sunitibala, Grosser, Jude W. and Dutt, Manjul等.  请登录

简介：Abiotic and biotic stresses adversely affect the growth and productivity of plants and constitute the major yield-limiting factor in agriculture. In recent years, several transcription factors (TF) have been identified that can function as switches to control the expression of potential stress-related genes. These transcription factors play crucial roles in governing transcriptional reprogramming ...

文献信息：Guerra-Lupian, M. A., Ruiz-Medrano, R., Ramirez-Pool, J. A., Ramirez-Ortega, F. A., Lopez-Buenfil, J. A., Loeza-Kuk, E., Morales-Galvan, O., Chavarin-等.  请登录

简介： Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and beta-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Chris...

文献信息：Hijaz, Faraj, Nehela, Yasser, Jones, Shelley E., Dutt, Manjul, Grosser, Jude W., Manthey, John A. and Killiny, Nabil等.  请登录

简介：Anthocyanins are synthesized via the flavonoid pathway through a complex expression of several genes such as MYB transcription factors. Anthocyanins protect plants against biotic and abiotic stresses. Herein, we studied the effect of expression of MYB (VvmybA1 cloned from the red grape and Ruby cloned from Moro' blood orange) transcription factors in Mexican lime on juice quality and leaf pigments...

文献信息：Miguel-Angel, Guerra-Lupián, Roberto, Ruiz Medrano, José-Abrahán, Ramírez-Pool, Francisco-Arturo, Ramírez-Ortega, José-Abel, López-Buenfil, Emiliano, 等.  请登录

简介：Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and β-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Christm....

文献信息： Kamiri Mourad, Stift Marc, Costantino Gilles, Dambier Dominique, Kabbage Tariq, Ollitrault Patrick, Froelicher Yann等.  2018-11-02 发表于【Frontiers in Plant Science】 | 请登录

简介：The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop k...

文献信息：Jia Ning, Liu Jiqin, Sun Yufeng, Tan Penghui, Cao Hao, Xie Yingying, Wen Boting, Gu Tianyi, Liu Jiameng, Li Minmin, Huang Yatao, Lu Jia, Jin Nuo, Sun 等.  发表于【Plant Science】 | 请登录

简介：Lignin is one of the most important components of the plant cell wall, and the expression and transcriptional regulation of lignin biosynthesis-related genes have been studied widely in Arabidopsis and other plants. Citrus fruit juice sacs often undergo lignification, particularly during fruit ripening and storage periods; however, the underlying genetic mechanisms have been little investigated. I...

文献信息：徐旋等.  请登录

简介：柑橘是世界产量第一的水果,由于童期长、种子多胚、某些重要的商业品种育性低甚至不育等特点,利用传统的育种方法改良柑橘进展缓慢。CRISPR/Cas9系统作为近几年快速发展的基因组编辑技术可以精确有效地诱导特定的突变,特异地改造基因组,已成为植物基因功能研究和作物遗传改良的重要手段之一。利用植物原生质体瞬时转化CRISPR/Cas9系统突变得到的植株,属于非转基因种质资源,能够省去漫长的后代筛选步骤;同时相对于农杆菌介导转化柑橘上...

文献信息：王晨阳等.  请登录

简介：microRNA(miRNA)是一类由内源基因编码的长度为21-23nt的非编码单链小RNA分子,在调控植物发育方面发挥着重要的作用。柑橘是世界第一大果树,其童期长、结果晚的特性严重影响了其经济效益和育种进程。已有大量研究报道表明,microRNA 156(miR156)和microRNA 172(miR172)参与调控植物成花转变。本研究前期通过转录组测序,共在枳中获得miR156家族3个成员,分别是PtmiR156a,PtmiR156b,PtmiR156c;以及miR172家族4个成员,分别是PtmiR172-03,P...

文献信息：童娅华等.  请登录

简介：铁(Fe)是植物生长发育必需的矿质营养,其参与植物新陈代谢、蛋白合成等多种生理生化过程,柑橘等嫁接植物主要通过地下部砧木吸收Fe营养。在柑橘生产中,枳因具有耐寒、抗脚腐病等诸多优良特性,普遍被用作柑橘砧木。但是,枳砧也存在不耐碱的缺点,以枳为砧木的柑橘园在碱性土上缺Fe严重,导致柑橘树体衰弱、产量锐减、品质下降,生产中也难以通过施肥等措施进行有效矫治。ZIP家族蛋白被认为是Zn、Fe、Mn、Cu等多种二价金属离子的重要转运子...

文献信息：胡思凡等.  请登录

简介：柑橘的童期较长,是阻碍柑橘育种的一个难题。童期结束植物方能由营养生长向生殖生长转变(即成花转变),才能正常进行开花结果,因此童期研究具有重要的生物学意义。植物在实现由营养生长向生殖生长转变的过程中,需感受一系列外界环境(温度、光照、激素等)的变化,存在着复杂的基因调控网络。有研究报道表明在模式植物拟南芥中FRI基因主要通过低温春化途径影响开花的。本实验室前期研究发现,枳花芽分化需要经历冬季低温才能正常进行,因此...

文献信息：侯康等.  请登录

简介：<br />Abstract: 柑橘是我国栽培面积最大的果树,其果实品质深受柑橘病害的影响,为减少化学药剂的使用,培育柑橘的抗病品种成为了柑橘病虫害防治的最有效最直接的选择。随着基因工程技术的发展,基因育种越来越受到人们的重视,对于柑橘抗病基因的研究也愈发深入。柑橘真菌性病害如柑橘褐斑病、炭疽病等对树体、果实都有巨大的影响,对我国柑橘产业造成了严重影响。Mlo基因是一类新发现的植物抗病基因家族,不同于传统抗病基因R基因的“基...

文献信息： 耿晶晶等.  请登录

简介：碱性螺旋-环-螺旋(b HLH)转录因子(TF)是植物最大的基因家族之一,广泛参与植物生长发育的多种生理过程,包括生物和非生物胁迫应答。目前,虽然在一些植物中已经鉴定出了b HLH基因并进行了研究,但主要集中在模式植物;大多数非模式植物,尤其是多年生果树b HLH转录因子所发挥的生理作用以及调控功能仍然不清楚。基于甜橙(Citrus sinensis)全基因组数据库信息,我们在全基因组水平上发掘甜橙b HLH家族基因,筛选低温响应的成员,并对受低温诱...

文献信息：白晓晶等.  请登录

简介：柑橘黄龙病(Huanglongbing,HLB)是世界性的柑橘检疫性病害,其病原菌为韧皮杆菌属(Liberibacter)的革兰氏阴性细菌,其中亚洲种(‘Candidatus Liberibacter asiaticus’,CLas)是黄龙病的主要致病变种。植物应对病原菌侵染防御机制中关键步骤是通过激活植物局部获得性抗性(Local acquired resistance,LAR)进一步引起系统获得性抗性(Systemic acquired resistance,SAR),这一过程的核心激活信号为水杨酸(Salicylic acid,SA)。植株产生SAR需...

文献信息：Duan Shuo, Jia Hongge, Pang Zhiqian, Teper Doron, White Frank, Jones Jeffrey, Zhou Changyong, Wang Nian等.  2018-08-01 发表于【 Molecular Plant Pathology】 | 请登录

简介： Xanthomonas citri ssp. citri (Xcc) is an important plant-pathogenic bacterium that causes citrus canker disease worldwide. PthA, a transcriptional activator-like (TAL) effector, directs the expression of the canker susceptibility gene CsLOB1. Here, we report our recent progress in the functional characterization of CsLOB1. Subcellular localization analysis of CsLOB1 protein in citrus protoplast r...

文献信息： Dutt Manjul, Erpen Ligia, Grosser Jude W.等.  2018-12-19 发表于【Scientia Horticulturae】 | 请登录

简介： Genetic transformation of citrus has been mainly conducted via Agrobacterium tumefaciens using juvenile tissues of the epicotyl segments obtained from the in vitro germination of seeds. Transformation of the stem segments obtained from the mature material is an alternative to overcome or reduce the juvenile phase reported in perennial species such as citrus. However, some citrus species, includin...

文献信息： Dutt M, Zambon F. T., Erpen L, Soriano L, Grosser J等.  发表于【Plos One】 | 请登录

简介：Abstract The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embry...

文献信息： Geng J., Liu J. H.等.  请登录

简介：CsbHLH18, a bHLH transcription factor ofCitrus sinensis,functions as a positive regulator of cold tolerance due to promoted ROS scavenging, and directly targets aPODgene. The basic helix–loop–helix (bHLH) transcription factors (TFs) comprise one of the largest gene families in plants, and participate in various physiological processes, but the physiological role and regulatory function of the ma...

文献信息：Romero-Romero J L, Inostroza-Blancheteau C, Orellana D, Aquea F, Reyes-Diaz M, Gil P M, Pablo Matte J, Arce-Johnson P等.  2018-09-01 发表于【Plant Physiology and Biochemistry】 | 请登录

简介：Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region,...

文献信息：Omar A A, Murata M M, El-Shamy H A, Graham J H, Grosser J W等.  2018-04-02 发表于【Transgenic Research】 | 请登录

简介：Genetic engineering approaches offer an alternative method to the conventional breeding of Citrus sp. 'W. Murcott' mandarin (a hybrid of 'Murcott' and an unknown pollen parent) is one of the most commercially important cultivars grown in many regions around the world. Transformation of 'W. Murcott' mandarin was achieved by direct DNA uptake using a protoplast transformation system. DNA construct (...