文献信息：童娅华等.  请登录

简介：铁(Fe)是植物生长发育必需的矿质营养,其参与植物新陈代谢、蛋白合成等多种生理生化过程,柑橘等嫁接植物主要通过地下部砧木吸收Fe营养。在柑橘生产中,枳因具有耐寒、抗脚腐病等诸多优良特性,普遍被用作柑橘砧木。但是,枳砧也存在不耐碱的缺点,以枳为砧木的柑橘园在碱性土上缺Fe严重,导致柑橘树体衰弱、产量锐减、品质下降,生产中也难以通过施肥等措施进行有效矫治。ZIP家族蛋白被认为是Zn、Fe、Mn、Cu等多种二价金属离子的重要转运子...

文献信息：胡思凡等.  请登录

简介：柑橘的童期较长,是阻碍柑橘育种的一个难题。童期结束植物方能由营养生长向生殖生长转变(即成花转变),才能正常进行开花结果,因此童期研究具有重要的生物学意义。植物在实现由营养生长向生殖生长转变的过程中,需感受一系列外界环境(温度、光照、激素等)的变化,存在着复杂的基因调控网络。有研究报道表明在模式植物拟南芥中FRI基因主要通过低温春化途径影响开花的。本实验室前期研究发现,枳花芽分化需要经历冬季低温才能正常进行,因此...

文献信息：侯康等.  请登录

简介：<br />Abstract: 柑橘是我国栽培面积最大的果树,其果实品质深受柑橘病害的影响,为减少化学药剂的使用,培育柑橘的抗病品种成为了柑橘病虫害防治的最有效最直接的选择。随着基因工程技术的发展,基因育种越来越受到人们的重视,对于柑橘抗病基因的研究也愈发深入。柑橘真菌性病害如柑橘褐斑病、炭疽病等对树体、果实都有巨大的影响,对我国柑橘产业造成了严重影响。Mlo基因是一类新发现的植物抗病基因家族,不同于传统抗病基因R基因的“基...

文献信息： 耿晶晶等.  请登录

简介：碱性螺旋-环-螺旋(b HLH)转录因子(TF)是植物最大的基因家族之一,广泛参与植物生长发育的多种生理过程,包括生物和非生物胁迫应答。目前,虽然在一些植物中已经鉴定出了b HLH基因并进行了研究,但主要集中在模式植物;大多数非模式植物,尤其是多年生果树b HLH转录因子所发挥的生理作用以及调控功能仍然不清楚。基于甜橙(Citrus sinensis)全基因组数据库信息,我们在全基因组水平上发掘甜橙b HLH家族基因,筛选低温响应的成员,并对受低温诱...

文献信息：白晓晶等.  请登录

简介：柑橘黄龙病(Huanglongbing,HLB)是世界性的柑橘检疫性病害,其病原菌为韧皮杆菌属(Liberibacter)的革兰氏阴性细菌,其中亚洲种(‘Candidatus Liberibacter asiaticus’,CLas)是黄龙病的主要致病变种。植物应对病原菌侵染防御机制中关键步骤是通过激活植物局部获得性抗性(Local acquired resistance,LAR)进一步引起系统获得性抗性(Systemic acquired resistance,SAR),这一过程的核心激活信号为水杨酸(Salicylic acid,SA)。植株产生SAR需...

文献信息：Duan Shuo, Jia Hongge, Pang Zhiqian, Teper Doron, White Frank, Jones Jeffrey, Zhou Changyong, Wang Nian等.  2018-08-01 发表于【 Molecular Plant Pathology】 | 请登录

简介： Xanthomonas citri ssp. citri (Xcc) is an important plant-pathogenic bacterium that causes citrus canker disease worldwide. PthA, a transcriptional activator-like (TAL) effector, directs the expression of the canker susceptibility gene CsLOB1. Here, we report our recent progress in the functional characterization of CsLOB1. Subcellular localization analysis of CsLOB1 protein in citrus protoplast r...

文献信息： Dutt Manjul, Erpen Ligia, Grosser Jude W.等.  2018-12-19 发表于【Scientia Horticulturae】 | 请登录

简介： Genetic transformation of citrus has been mainly conducted via Agrobacterium tumefaciens using juvenile tissues of the epicotyl segments obtained from the in vitro germination of seeds. Transformation of the stem segments obtained from the mature material is an alternative to overcome or reduce the juvenile phase reported in perennial species such as citrus. However, some citrus species, includin...

文献信息： Dutt M, Zambon F. T., Erpen L, Soriano L, Grosser J等.  发表于【Plos One】 | 请登录

简介：Abstract The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embry...

文献信息： Geng J., Liu J. H.等.  请登录

简介：CsbHLH18, a bHLH transcription factor ofCitrus sinensis,functions as a positive regulator of cold tolerance due to promoted ROS scavenging, and directly targets aPODgene. The basic helix–loop–helix (bHLH) transcription factors (TFs) comprise one of the largest gene families in plants, and participate in various physiological processes, but the physiological role and regulatory function of the ma...

文献信息：Romero-Romero J L, Inostroza-Blancheteau C, Orellana D, Aquea F, Reyes-Diaz M, Gil P M, Pablo Matte J, Arce-Johnson P等.  2018-09-01 发表于【Plant Physiology and Biochemistry】 | 请登录

简介：Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region,...

文献信息：Omar A A, Murata M M, El-Shamy H A, Graham J H, Grosser J W等.  2018-04-02 发表于【Transgenic Research】 | 请登录

简介：Genetic engineering approaches offer an alternative method to the conventional breeding of Citrus sp. 'W. Murcott' mandarin (a hybrid of 'Murcott' and an unknown pollen parent) is one of the most commercially important cultivars grown in many regions around the world. Transformation of 'W. Murcott' mandarin was achieved by direct DNA uptake using a protoplast transformation system. DNA construct (...

文献信息： Lu Pengjun, Wang Shasha, Grierson Don, Xu Changjie等.  发表于【Planta】 | 请登录

简介：Carotenoids are important plant pigments and their accumulation can be affected by biosynthesis inhibitors, but the genes involved were largely unknown. Here, application of norflurazon (NFZ), 2-(4-chlorophenylthio)-triethylamine hydrochloride (CPTA) and clomazone for 30 days to in vitro cultured sweet orange juice vesicles caused over-accumulation of phytoene (over 1000-fold), lycopene (2.92 μg ...

文献信息：Liu Z, Ge X-X, Qiu W-M, Long J-M, Jia H-H, Yang W, Dutt M, Wu X-M, Guo W-W等.  2018-12-01 发表于【Plant Science】 | 请登录

简介：In citrus, genetic improvement via biotechnology is challenging due to insufficient understanding of molecular barriers that prevent regeneration by somatic embryogenesis (SE). Our previous study indicated that LEC genes were involved in SE in citrus, but their regulatory roles remain to be elucidated. Here, we cloned one of the LEC genes, CsFUS3, and show that it is preferentially expressed durin...

文献信息： 张梦原, 谢幸男, 李盈, 葛晓霞等.  发表于【园艺学报】 | 请登录

简介：以‘伏令夏橙’叶片基因组DNA为模板,克隆了CsHB1启动子5′端转录起始位点上游分别为1 741 bp和1 613 bp的区域序列。序列分析表明这两个启动子区序列相似度为90.7%,存在141 bp的差异片段,该差异片段中含有2个Box4元件。通过PlantCare数据库对启动子序列的顺式作用元件进行预测,结果表明CsHB1的不同启动子序列含有多种响应元件,如胚乳特异性元件、分生组织表达元件、光反应元件、热应激反应元件、MYB结合位点等。为进一步分析CsHB1启...

文献信息：唐艳, 陈传武, 付慧敏, 伊华林, 邓崇岭, 邓光宙, 刘冰浩 and 李丁凤等.  发表于【果树学报】 | 请登录

简介：‘桂夏橙1号’是从‘阿尔及利亚夏橙’芽变中选育的柑橘新品种。果实圆球形或椭圆形,果皮橙黄色,果面较粗糙,果顶圆,果肉橙黄色,味酸甜,多汁,有香气;果大,单果质量197.8~259.7 g,果实横径71.0~80.3 mm,纵径68.6~80.1 mm,果形指数0.94~1.03;种子数平均1.68粒,一般1~3粒。可食率70.28%~76.47%,果汁率44.99%~54.39%,可溶性固形物含量(ω)10.2%~13.3%,每100 m L果汁中含维生素C 45.96~55.04 mg,柠檬酸0.68~1.20 g,全糖7.18~9.65 g,固酸比...

文献信息：李益, 马先锋, 唐浩, 李娜, 江东, 龙桂友, 李大志, 牛英, 韩瑞玺 and 邓子牛等.  发表于【中国农业科学】 | 请登录

简介：【目的】筛选出一套SSR核心引物,用于构建柑橘品种的DNA指纹图谱库,为柑橘种苗认证、品种登记和植物新品种保护提供技术支撑。【方法】以柑橘属8个主要栽培种类为试材进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测,筛选出多态性丰富、扩增条带稳定的引物对部分参试品种进行PCR扩增,扩增产物通过变性聚丙烯酰胺凝胶电泳进行进一步的引物筛选;筛选得到的引物进行荧光标记,并选用部分柑橘品种进行PCR扩增,扩增产物利用基因分析仪检测,分别计...

文献信息：Yu, Jihua, Zhang, Minrong, Zhong, Liequan, Yang, Xiao, Li, Ping, Lu, Lu, Zhang, Ning and Tao, Jian等.  发表于【 Journal of Agriculture】 | 请登录

简介：This paper mainly reviewed some researches on prevention and control of Candidatus Liberibacter asiaticus and Diaphorina citri in Taizhou from 2002 to 2017. It was found that the population of citrus HLB was distributed evenly in citrus orchards at the early stage of invasion, but subsequently developed in the pattern of aggregation. Accordingly, the population of Diaphorina citri belonged to the ...

文献信息：Xiu-Li, X. I., Huang, Hai Bo, Lou, Bu Qing, Zhan, Ruo Ting and Wang, Hao Hang等.  发表于【 Chinese Traditional & Herbal Drugs】 | 请登录

简介： Objective To carry out genetic polymorphism analysis of Citrus reticulata Blanco cv. chachiensis Tanaka and its relatives by using SCoT molecular marker method. Methods Five factors of Mg2+, d NTPs, Taq DNA polymerase, primer, and template DNA concentration were used to screen the suitable SCoT-PCR reaction system for C. reticulata and its relatives by the method of orthogonal design. The optimum...

文献信息： Usman, Muhammad and Fatima, Bilquees等.  请登录

简介：The mandarin horticultural group is an important component of world citrus production for the fresh fruit market. This group formerly classified as C. reticulata is highly polymorphic and recent molecular studies have suggested that numerous cultivated mandarins were introgressed by C. maxima (the pummelos). C. maxima and C. reticulata are also the ancestors of sweet and sour oranges,... [Show ful...

文献信息：Shahid, Muhammad Adnan, Balal, Rashad Mukhtar, Khan, Naeem, Zotarelli, Lincoln, Liu, Guodong, Ghazanfar, Muhammad Usman, Rathinasabapathi, Bala, Matts等.  发表于【Environmental & Experimental Botany】 | 请登录

简介： This study compared the effects of chromium toxicity on the carbon and nitrogen metabolism in Kinnow mandarin ( Citrus nobilis Lour x Citrus deliciosa Ten) plants grafted on the diploid (2x) and tetraploid (4x) of Poncirus trifoliata [L.], Citrus reshni , and Citrus limonia Osbeck. Plants were grown under controlled conditions and fertigated with complete nutrient solution supplemented with chrom...