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  • 321. Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

    文献信息:Peng A, Chen S, Lei T, Xu L, He Y, Wu L, Yao L, Zou X等.  2017-12-01 发表于【Plant Biotechnology Journal】 | 请登录

    简介:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR/Cas9-targeted modification of the susceptibility gene CsLOB1 pro...

    322. 柑橘转录因子CsCBF1和PtrNAC72在调控精氨酸脱羧酶基因表达及抗逆中的功能鉴定

    文献信息: 吴昊等.  请登录

    简介:柑橘是世界上栽培最广、产量最高的水果作物,其中甜橙所占的比例最大,年产量约为柑橘类水果总产量的60%。甜橙喜温不耐寒,因此低温是限制柑橘产量的主要环境因素之一。多胺是含有两个或两个以上氨基的脂肪族化合物,参与植物生长发育、组织分化、果实成熟与衰老以及抵抗生物和非生物胁迫等重要生理过程,在一定程度内,多胺在细胞中的积累量越多,其胁迫抗性越强。而在逆境胁迫下多胺合成酶基因表达量的上调是多胺在细胞中积累的前提,因此...

    323. Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing

    文献信息:Xu, X., Xu, X., Zhou, Y., Zeng, S. and Kong, W.等.  发表于【Plos One】 | 请登录

    简介:Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts. MicroRNAs (miRNAs) play important roles in plant growth, development and stress responses. However, the underlying mechanism of miRNAs involved in the protoplast totipotency remains uncle...

    324. Optimization of an in vitro Regeneration Protocol for Rough Lemon Rootstock (Citrus jambhiri L.) via Direct Organogenesis

    文献信息:Taye, Molla Gereme, Debesay, Brhanu, Tesfahun, Yikunoamilak and Brhanu, Assefa等.  请登录

    简介:Optimization of an in vitro Regeneration Protocol for Rough Lemon Rootstock (Citrus jambhiri L.) via Direct Organogenesis Abstract.

    325. Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange ( Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease

    文献信息:Sendín, Lorena Noelia, Orce, Ingrid Georgina, Gómez, Rocío Liliana, Enrique, Ramón, Noguera, Aldo Sergio, Vojnov, Adrián Alberto, Marano, María Rosa, 等.  发表于【Plant Molecular Biology】 | 请登录

    简介:: Abstract Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc)...

    326. Tetraploid Carrizo citrange rootstock ( Citrus sinensis Osb. × Poncirus trifoliata L. Raf.) enhances natural chilling stress tolerance of common clementine ( Citrus clementina Hort. ex Tan)

    文献信息:Oustric, Julie, Morillon, Raphaël, Luro, François, Herbette, Stéphane, Lourkisti, Radia, Giannettini, Jean, Berti, Liliane and Santini, Jéré等.  发表于【Journal of Plant Physiology Volume: 214】 | 请登录

    简介: Low temperatures can disturb the development, growth and geographic distribution of plants, particularly cold-sensitive plants in the Mediterranean area, where temperatures can reach seasonally low levels. In citrus crops, scion/rootstock combinations are used to improve fruit production and quality, and increase tolerance to biotic and abiotic stresses. In the last decade, several studies have s...

    327. Genetic identification and inference on genetic relationships of important Citrus rootstocks with microsatellite markers

    文献信息: Naliath, Ritu, Kumar, Krishan, Arora, Parshotam Kumar, Kaur, Satinder, Kaur, Dasmeet and Singh, Kuldeep等.  发表于【Fruits】 | 请登录

    简介:Twenty-seven (27) grape genotypes representing different grape groups namely table, wine, juice grapes and rootstocks with present or potential future importance for sub-tropical viticulture were characterized with 29 microsatellite markers spanning over 15 linkage groups of ‘Syrah’  ‘Grenache’ linkage map. The objective of the investigation was to establish the unique molecular identi...

    328. Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect

    文献信息:Martinezcuenca, M. R., Primocapella, A., Quiñones, A., Bermejo, A. and Fornerginer, M. A.等.  发表于【Peerj】 | 请登录

    简介:Abstract Background and aims This work evaluates the regulation of iron uptake responses in Citrus leaves and their involvement in the Fe paradox effect. Methods Experiments were performed in field-grown ‘Navelina’ trees grafted onto two Cleopatra mandarin × Poncirus trifoliata (L.) Raf. hybrids with different Fe-chlorosis symptoms: 030146 (non-chlorotic) and 030122 (chlorotic). Results Chlorot...

    329. Transcriptome Profiling to Understand the Effect of Citrus Rootstocks on the Growth of ‘Shatangju’ Mandarin

    文献信息:Liu, X. Y., Li, J., Liu, M. M., Yao, Q. and Chen, J. Z.等.  发表于【Plos One】 | 请登录

    简介:To obtain insight into potential mechanisms underlying the influence of rootstock on scion growth, we performed a comparative analysis of ‘Shatangju’ mandarin grafted onto 5 rootstocks: Fragrant orange (Citrus junonsSieb. ex. Tanaka), Red tangerine (Citrus reticulataBlanco), ‘Shatangju’ mandarin (Citrus reticulataBlanco), Rough lemon (Citrus jambhiriLush) and Canton lemon (Citrus limoniaOsbeck...

    330. \'Candidatus Liberibacter asiaticus\' Encodes a Functional Salicylic Acid (SA) Hydroxylase That Degrades SA to Suppress Plant Defenses

    文献信息: Li, J., Pang, Z., Trivedi, P, Zhou, X., Ying, X., Jia, H. and Wang, N.等.  发表于【Molecular plant】 | 请登录

    简介:Abstract Pathogens from the fastidious, phloem-restricted \'Candidatus Liberibacter\' species cause the devastating Huanglongbing (HLB) disease in citrus worldwide and cause diseases on many solanaceous crops and plants in the Apiaceae family. However, little is known about the pathogenic mechanisms due to the difficulty in culturing the corresponding Liberibacters. Here we report that the citrus ...

    331. Rootstocks for the grapefruit hybrid “Sweetie” (‘Oroblanco’) under organic and conventional management

    文献信息:Klein, Joshua D., Shalev, Yonit Raz, Cohen, Shlomo and Sachs, Moshe等.  发表于【 Scientia Horticulturae】 | 请登录

    简介:The yield (kg ha −1 ) and quality (weight, total soluble solids (TSS%), TSS:acid ratio, antioxidative activity in juice and peel) of “Sweetie” ( Citrus maxima × C. paradisi , also known as oroblanco) fruit grown on the non-traditional ‘Poorman’s Orange’ rootstock were similar to and often better than that of “Sweetie” grown on the commercial standard sour orange rootstock, which is...

    332. Ectopic expression of Xylella fastidiosa rpfF conferring production of diffusible signal factor in transgenic tobacco and citrus alters pathogen behavior and reduces disease severity

    文献信息:Somatic embryogenesis is an important in vitro technique used to obtain Citrus sinensis(L.) Osbeck (sweet orange) plantlets for conservation, sanitati等.  发表于【Molecular plant】 | 请登录

    简介:react-text: 177 Investigations of biofilm resistance response rarely focus on plant-pathogenic bacteria. Since Xylella fastidiosa is a multihost plant-pathogenic bacterium that forms biofilm in the xylem, the behavior of its biofilm in response to antimicrobial compounds needs to be better investigated. We analyzed here the transcriptional profile of X. fastidiosa subsp. pauca in response to inhib...

    333. Somatic embryogenesis of a seedless sweet orange ( Citrus sinensis (L.) Osbeck) Journal: In Vitro Cellular & Developmental Biology - Plant

    文献信息:Cardoso, Jean C., Curtolo, Maisa, Latado, Rodrigo R. and Martinelli, Adriana P.等.  请登录

    简介:Somatic embryogenesis is an important in vitro technique used to obtain Citrus sinensis(L.) Osbeck (sweet orange) plantlets for conservation, sanitation, propagation, and breeding. The induction of...

    334. Efficient propagation of citrus rootstocks by stem cuttings

    文献信息: Bowman, K. D. and Albrecht, U.等.  发表于【Scientia Horticulturae】 | 请登录

    简介: A simple multicomponent system is described that is effective for rapid propagation of a diversity of citrus rootstock genotypes by single node cuttings, including new hybrids and those that are most commonly used as rootstocks. Efficiency of this system for rooting shoot explants of six important rootstock cultivars, Swingle, Cleopatra, US-802, US-812, US-897, and US-942 is compared in a repeate...

    335. Accurate measurement of transgene copy number in crop plants using droplet digital PCR

    文献信息:Collier, Ray Dasgupta, Kasturi Xing, Yan‐\Ping Hernandez, Bryan Tarape Shao, Min Rohozinski, Dominica Kovak, Emma Lin, Jeanie De Oliveira, Maria Luiza等.  2017-02-23 发表于【Plant Journal】 |  , 请登录

    简介:Technical abstract: Genetic transformation is a powerful means for the improvement of crop plants, but requires labor and resource intensive methods. An efficient method for identifying single copy transgene insertion events from a population of independent transgenic lines is desirable. Currently transgene copy number is estimated by either Southern blot hybridization analyses or quantitative pol...

    336. Ectopic accumulation of linalool confers resistance to Xanthomonas citri subsp citri in transgenic sweet orange plants

    文献信息:Shimada, Takehiko Endo, Tomoko Rodriguez, Ana Fujii, Hiroshi Goto, Shingo Matsuura, Takakazu Hojo, Yuko Ikeda, Yoko Mori, Izumi C. Fujikawa, Takashi P等.  2017-06-28 发表于【Tree Physiology】 |  , 请登录

    简介:In order to clarify whether high linalool content in citrus leaves alone induces strong field resistance to citrus canker caused by Xanthomonas citri subsp. citri (Xcc), and to assess whether this trait can be transferred to a citrus type highly sensitive to the bacterium, transgenic \'Hamlin\' sweet orange (Citrus sinensis L. Osbeck) plants over-expressing a linalool synthase gene (CuSTS3-1) were...

    337. Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

    文献信息:Peng, Aihong Chen, Shanchun Lei, Tiangang Xu, Lanzhen He, Yongrui Wu, Liu Yao, Lixiao Zou, Xiuping等.  2017-03-21 发表于【Plant biotechnology journal】 |  , 请登录

    简介:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR/Cas9-targeted modification of the susceptibility gene CsLOB1 pro...

    338. Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions

    文献信息:Martins, C. P. Neves, D. M. Cidade, L. C. Mendes, A. F. Silva, D. C. Almeida, A. F. Coelho-Filho, M. A. Gesteira, A. S. Soares-Filho, W. S. Costa, M. 等.  2017-01-21 发表于【Planta】 |  , 请登录

    简介:Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing di...

    339. 柑橘新品种‘桂野生山金柑’的选育

    文献信息:李果果, 麦彩胜 ,刘要鑫, 柴利军 ,陈香玲 ,赵小龙, 欧智涛, 张兰 ,赵洪涛,等.  2017-03-01 发表于【果树学报】 |  , 请登录

    简介:‘桂野生山金柑’是在广西壮族自治区东兴市发现的金柑属柑橘新品种。果实近圆形,成熟时果皮黄色,果面光滑,有蜡层,肉质紧密,风味显酸,平均单果质量4.5 g,果形指数0.98,种子1~7粒,卵形,深绿色,单胚。果实可食率87.1%,果实可溶性固形物含量10.6%,每L果汁中含可滴定酸45 g和维生素C 374 mg。该品种在广西东兴地区11月初成熟,花单生或簇生,萌芽力和成枝力弱,抗逆性和抗病性中等。适合广西地区栽培,幼树第2年可开花坐果。

    340. 柑橘砧木新品种‘蒲江香橙’的选育

    文献信息:伏晓科, 黄晓姣, 陈涛, 张静 ,王燕, 陈清, 雷清良 ,汤浩茹, 王小蓉等.  2017-05-01 发表于【果树学报】 |  , 请登录

    简介:蒲江香橙’是从四川省蒲江县白云乡实生野生香橙中经过多年观察和试验比较选择育成的柑橘砧木新品种。作为砧木,该品种树势强健,树冠圆头形,植株高大,枝条较硬、直立而密生、伸展。果实椭圆近扁圆形,果皮橙黄色,单果质量78.1~112.8 g,单果种子数32~43粒,平均37.5粒,种子质量占果实的10.1%左右,盛年期每株树可产种子3.5~5.5 kg。用作柑橘砧木,嫁接亲和性好,接穗品种早结、丰产、稳产,果实品质优良,遗传性状稳定。抗寒、抗涝、耐碱性土...

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