文献信息：Dutt M, Barthe G.A, Orbovic V, Irey M, Grosser J.W等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Transgenic citrus scion and rootstock cultivars were transformed with gene(s) encoding antimicrobial peptides or systemic acquired resistance (SAR) proteins. Each gene was under control of an enhanced CaMV 35S promoter. Several genes were also under control of a phloem specific Arabidopsis SUC2 (AtSUC2) promoter. A number of clones of each transgenic line were evaluated for resistance to Huanglong...

文献信息：Oliveri C, Distefano G, La Malfa S, La Rosa R, Deng Z, Gentile A.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents an efficient strategy to confer genetic resistance against a broad range of phytopathogenic fungi. In lemon (Citrus limon), fungal diseases cause economic losses in the field as well as in postharvest management. In this study, fruits collected from two different transgenic lemon cl...

文献信息：Castro LM, Mendes BMJ, Mourão Filho FAA等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：The use of mature tissue for citrus genetic transformation is important for the precocious evaluation of the genetically modified characteristics. However, an efficient organogenesis protocol with high rates of plant regeneration is necessary. This work aimed to optimize a protocol for in vitro organogenesis of ‘Hamlin’, ‘Pera’, and ‘Valencia’ sweet orange cultivars (Citrus sinensis) using a...

文献信息：Tallón CI, Porras I, Pérez-Tornero O.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：The influence of the basal medium and different plant growth regulators on the efficient micropropagation of nodal explants from mature trees of sour orange and ‘Cleopatra’ mandarin citrus rootstocks was studied. The preference of all two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed. Several combinations of BA and AD, KIN or GA were made in an attempt to opti...

文献信息：Xie YM, Yang L, Hu W, Li Y, Deng ZN.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：High level of contamination, low morphogenetic potential and recalcitrance to Agrobacterium tumefaciens infection are the main problems in the transformation of adult citrus tissues. A series of experiments was performed to solve these problems. ‘Newhall’, ‘Succari’ and ‘Bingtang’ sweet orange cultivars were tested. Adult shoots from the 3 cultivars were grafted and re-grafted on Poncirus tr...

文献信息：Yang L, Hu W, Xie YM, Li Y, Deng ZN.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Internodal stem segments from citrus seedlings have been widely used as explants in transformation research, due to their ability of easy regeneration. Since 1990, genetic transformation has been applied on different citrus species including sweet oranges (Citrus sinensis), mandarins (C. reticulata), trifoliate orange (Poncirus trifoliata), sour orange (C. aurantium), pummelo (C. grandis), citrang...

文献信息：Jensen SP, Febres VJ, Moore GA.等.  请登录

简介：Citrus transformation is typically Agrobacterium tumefaciens-mediated, in which citrus tissues are cultivated with the bacterium and regenerated on selection media. Part of the bacterial plasmid, with the gene of interest and resistance genes, is then inserted into the citrus genome. Due to the slow growth of citrus, this long process must be optimized for each cultivar and ultimately the transfor...

文献信息：Pons E, Navarro A, Ollitrault P, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Despite potential benefits granted by genetically modified (GM) citrus trees, their release and commercialization raises concerns about their potential environmental impact. The transfer via pollen of transgenes to crosscompatible cultivars is deemed to be the greatest source for environmental exposure. In this work, three different citrus genotypes carrying the uidA (GUS) tracer marker gene (poll...

文献信息：Zou XP, Peng AH, Liu XF, He YR, Xu LZ, Chen SC.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Selectable marker genes conferring antibiotic- or herbicide- resistance are in general required to efficiently recover transgenic plants from transformed cells during plant transformation. However, the presence of marker genes raises public concerns regarding the field release of transgenic plants. Removal of marker genes prevents the risk of its release into the environment and hastens the public...

文献信息：Soler N, Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña L. 等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus tristeza virus (CTV) is phloem-restricted in natural citrus hosts. The 23 kDa protein (p23) encoded by the virus is a RNA silencing suppressor and an important pathogenicity factor. Constitutive expression of p23 in transgenic citrus plants under the control of the 35S promoter incites, in addition to aberrations resembling CTV symptoms, others likely related to the ectopic expression of th...

文献信息：Marques VV, Miller AM, Bagio TZ, Souza GV, Meneguin L, Vieira LGE, Pereira LFP, Leite JRRP.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus Canker caused by Xanthomonas citri subsp. citri (Xcc) is an important disease for citrus production around the world. Several highly valuable commercial citrus cultivars are moderately to highly susceptible to this disease. Development of transgenic plants for disease resistance in economically important citrus cultivars may represent a unique and efficient strategy for control of Citrus Ca...

文献信息：Peris JE, Vicent A, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Phytophthora spp. causes foot rot and gummosis, the most serious soilborne diseases of citrus worldwide. Sweet orange (Citrus sinensis) might be an excellent rootstock but it cannot be used as such in many citrus areas due to its high sensitivity to Phytophthora spp. The development of sweet orange that possess durable genetic resistance provides the best prospect for full recovery of this citrus ...

文献信息：Jin SB, Sun HJ, Kim YW, Bachchu MAA, Yun SH, Riu KZ, Kim JH.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：An efficient system for the production of a recombinant miraculin protein in transgenic citrus plant cells was developed. Miraculin is a taste-modifying protein that can transform a sour taste into a sweet taste. A miraculin gene containing a N-terminal signal peptide was introduced into navel orange (Citrus sinensis) callus cells by Agrobacterium-mediated transformation. Transgenic green color so...

文献信息：Muniz FR, Harakava R, Rezende JAM, Moore GA, Febres VJ, Mourao Filho FAA, Mendes BMJ.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus tristeza virus (CTV) control relies mainly on the use of tolerant rootstocks and scion cross protection. The aim of this work was to evaluate transgenic C. sinensis lines for resistance to CTV, in order to offer another alternative for virus control. Transgenic lines of C. sinensis cv. ‘Valencia’ and ‘Hamlin’ containing one of 3 different genetic constructs (pCTV-CP: coat protein gene o...

文献信息：Kava-Cordeiro V, Rodríguez A, Vicent A, Glienke C, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus Black Spot (CBS) is a disease affecting sweet orange, lemons and mandarin varieties in Africa, Oceania and Americas. In Europe, CBS is a quarantine disease, limiting the import of fresh fruits from citrus-producing countries of those continents. CBS is caused by the fungus Phyllosticta citricarpa, which induces damages in fruit flavedo affecting their appearance and additionally causing pre...

文献信息：Tavano ECR, Harakava R, Vieira MLC, Mourão Filho FAA, Mendes BMJ.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Huanglongbing (HLB) associated with sieve tube-restricted bacteria Candidatus Liberibacter spp., is one of the most destructive disease affecting citrus. The aim of this study was to produce transgenic sweet orange plants (Citrus sinensis) expressing antimicrobial attacin A gene (attA) under the control of phloem tissue specific promoters, in order to obtain resistant plants to Candidatus Liberiba...

文献信息：Alquézar B, Pons E, Rodríguez A, Rodrigo M.J, Zacarías L, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Orange fruit is an excellent natural dietary source of phytochemicals and nutrients (as vitamin C, carotenoids, flavonoids and folic acid, among others) contributing to its quality, nutritional value and health benefits. In recent years, considerable advances in the understanding of the molecular basis regulating carotenoid biosynthesis have been accomplished and biotechnological strategies to mod...

文献信息：Rodríguez A, Cervera M, Peña L.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus trees have a long juvenile phase that delays their reproductive development by more than 6 years. This represents a major obstacle for citrus breeding. The key genes regulating floral transition have been studied extensively in species such Arabidopsis thaliana and poplar. In recent years, a growing number of genes related to flowering have been identified in crop species. With the aim of a...

文献信息：Cao HB, Zhang JC, Xu JD, Ye JL, Yun Z, Xu Q, Xu J, Deng XX.等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Citrus fruits, which exhibit extensive diversity of carotenoid patterns, can serve as a unique system to improve the understanding of carotenogenesis. Herein, we engineered embryogenic calli from four citrus genotypes as engineered cell models (ECMs) by overexpressing a bacterial phytoene synthase gene (CrtB). Ripe flavedos (the colored outer layer of citrus fruits) were offered as a comparative s...

文献信息：Carlos EF, Ariyoshi C, Felício MS, Shimizu P, Souza GV, Kudo SA, Silva LM, Silva AJM, Cação SMB, Vieira LGE等.  发表于【Ⅶ International Citrus Congress】 | 请登录

简介：Most of the citrus trees grown in Brazil are extensively managed with no irrigation, leaving trees more vulnerable to extended dry season, which may reach 90 days without rain in some areas. Thus, utilization of rootstocks more tolerant to drought, such as ‘Rangpur’ lime (Citrus limonia), is widely employed, while other rootstocks are not. Our laboratory has produced ‘Carrizo’ and ‘Swingle’ ...