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  • 21. Gene silencing of cathepsins B and L using CTV-based, plant-mediated RNAi interferes with ovarial development in Asian citrus psyllid (ACP), Diaphorina citri Journal(期刊名称): Frontiers in plant science

    文献信息:Ibanez Freddy, Vieira Rocha S mara, Dawson William O,El-Mohtar Choaa,Robertson Cecile,Stelinski Lukasz L,Soares-Costa Andrea等.  请登录

    简介:Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a vector of the bacteria Candidatus Liberibacter americanus (CLam) and Candidatus Liberibacter asiaticus (CLas), which are phloem-restricted and associated with the most important and destructive worldwide citrus disease, Huanglongbing (HLB). Currently, no cure for HLB has been described. Therefore, measures have focused on reducing D. citri popul...

    22. Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy

    文献信息:Huang, Xiaoen, Jia, Hongge, Xu, Jin, Wang, Yuanchun, Wen, Jiawen and Wang, Nian等.  请登录

    简介:Transgene-free plant genome editing in the T0 generation is highly desirable but challenging1,2. Here we achieved such a goal using a co-editing strategy via Agrobacterium-mediated transient expression of cytosine base editor to edit ALS encoding acetolactate synthase to confer herbicide chlorsulfuron resistance as a selection marker, Cas12a/CRISPR RNA for editing gene(s) of interest, and green fl...

    23. Transposable elements cause the loss of self-incompatibility in citrus

    文献信息:Hu Jianbing, Liu Chenchen, Du Zezhen,Guo Furong,Song Dan,Wang Nan,Wei Zhuangmin,Jiang Jingdong,Cao Zonghong,Shi Chunmei,Zhang Siqi,Zhu Chenqiao,Chen P等.  请登录

    简介:Self-incompatibility (SI) is a widespread prezygotic mechanism for flowering plants to avoid inbreeding depression and promote genetic diversity. Citrus has an S-RNase-based SI system, which was frequently lost during evolution. We previously identified a single nucleotide mutation in Sm -RNase, which is responsible for the loss of SI in mandarin and its hybrids. However, little is known about oth...

    24. Identification of Late Ripening Citrus Mutant, Ara-unshiu (Citrus unshiu), and Its Selectable Marker

    文献信息:Heo Ji-Man, Eun Chang-Ho, Kim In-Jung等.  请登录

    简介:Miyagawa-wase mandarin (Citrus unshiu Marc. cv. Miyagawa-wase early) is one of the most widely cultivated citrus varieties on Jeju Island in Korea. Mutation breeding is a useful tool for the induction of genetic diversity for the rapid creation of new plant variants. We previously reported the use of gamma irradiation for the development of new citrus varieties. Here, we report a new mutant, Ara-u...

    25. Production of marker-free transgenic plants from mature tIssue(期)s of navel orange using a Cre/loxP site-recombination system

    文献信息:He, Yongrui, Xu, Lanzhen, Peng, Aihong, Lei, Tiangang, Li, Qiang, Yao, Lixiao, Jiang, Guojin, Chen, Shanchun, Li, Zhengguo and Zou, Xiuping等.  请登录

    简介: Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor. Here, we constructed an Agrobacterium tumefaciens-mediated transformation for generation of marker-free transgenic plants from navel orange (Citrus sinensis Osbeck) mature stems usi...

    26. Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line

    文献信息:Fang, Hong, Culver, James N., Niedz, Randall P. and Qi, Yiping等.  请登录

    简介:Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a powerful genome editing tool. Recently developed CRISPR-Cas12a system confers several advantages over CRISPR-Cas9, making it ideal for use in plant genome editing and crop improvement. While traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and off-targe...

    27. The Expansin Gene CsLIEXP1 Is a Direct Target of CsLOB1 in Citrus

    文献信息:de Souza-Neto, Reinaldo Rodrigues, da Costa Vasconcelos, Fernanda Nogales, Teper, Doron, Barbosa Carvalho, Isis Gabriela, Takita, Marco Aurelio, Bened等.  请登录

    简介:Transcription activator-like effectors are key virulence factors of Xanthomonas. They are secreted into host plant cells and mimic transcription factors inducing the expression of host susceptibility (S) genes. In citrus, CsLOB1 is a direct target of PthA4, the primary effector associated with citrus canker symptoms. CsLOB1 is a transcription factor, and its expression is required for canker sympt...

    28. Comparative Analysis of Physicochemical Properties and Storability of a New Citrus Variety, Yellowball, and Its Parent

    文献信息:Comparative Analysis of Physicochemical Properties and Storability of a New Citrus Variety, Yellowball, and Its Parent等.  请登录

    简介:Although numerous citrus varieties have recently been developed to enhance their quality, information on their quality characteristics is limited. We assessed the quality characteristics of Yellowball, a novel citrus variety, by evaluating its appearance, storability, sensory properties, functionality, and metabolite profiles and then comparing these characteristics with those of its parent variet...

    29. Small RNAs contribute to citrus Huanglongbing tolerance by manipulating methyl salicylate signaling and exogenous methyl salicylate primes citrus groves from emerging infection

    文献信息:Cheng, Baoping, Xu, Le,Bilal, Muhammad Saqib,Huang, Qing,Niu, Dongdong,Ma, Hongyu,Zhou, Shaoxia,Peng, Aitian,Wei, Guo,Chen, Feng,Zeng, 等.  请登录

    简介: Citrus production is severely threatened by the devastating Huanglongbing (HLB) disease globally. By studying and analyzing the defensive behaviors of an HLB-tolerant citrus cultivar Shatangju, we discovered that citrus can sense Candidatus Liberibacter asiaticus (CLas) infection and induce immune responses against HLB, which can be further strengthened by both endogenously produced and exogenous...

    30. Influence of Anthocyanin Expression on the Performance of Photosynthesis in Sweet Orange, Citrus sinensis (L.) Osbeck

    文献信息:Cheaib Alissar, Mahmoud Lamiaa M, Vincent Christopher,Killiny Nabil,Dutt Manjul等.  请登录

    简介:Anthocyanins are a class of natural pigments that accumulate transiently or permanently in plant tIssue(期)s, often in response to abiotic and biotic stresses. They play a photoprotective role by attenuating the irradiance incident on the photochemical apparatus and quenching oxyradicals through their powerful anti-oxidative function. The objective of the current study is to understand the impac...

    31. Suppression of citrus canker disease mediated by flagellin perception

    文献信息:Andrade, Maxuel de Oliveira, da Silva, Jaqueline Cristina, Soprano, Adriana Santos, Shimo, Hugo Massayoshi, Leme, Adriana Franco Paes and Benedetti, C等.  请登录

    简介:Citrus cancer, caused by strains of Xanthomonas citri (Xc) and Xanthomonas aurantifolii (Xa), is one of the most economically important citrus diseases. Although our understanding of the molecular mechanisms underlying citrus canker development has advanced remarkably in recent years, exactly how citrus plants fight against these pathogens remains largely unclear. Using a Xa pathotype C strain tha...

    32. Discovery and characterization of magnesium transporter (MGT) gene family in Citrus sinensis and their role in magnesium deficiency stress

    文献信息: Bin, Minliang, Yi, Ganjun and Zhang, Xinxin等.  请登录

    简介:The divalent cation Magnesium (Mg2+) is necessary for healthy plant development, growth, and defense. Plants acquire Mg2+ from the soil and transport it throughout their body tissues with Mg2+ transporter (MGT) proteins. Although several MGT genes have been discovered and described in rice, maize, and Arabidopsis, far less information is available regarding their diversity and function in other pl...

    33. A novel bud mutant of navel orange (Citrus sinensis) shows tolerance to chlorosis in acidic and magnesium-deficient soils

    文献信息:Chaudhry, Ahmad Hassan, Hussain, Syed Bilal, Du, Wei, Liu, Yongzhong, Peng, Shu-Ang, Deng, Xiuxin and Pan, Zhiyong等.  请登录

    简介:Interveinal chlorosis in old leaves is a common occurrence in citrus orchards in southern China. The present study investigates the 'Langfeng' navel orange (LF, Citrus sinensis) grafted onto a Trifoliate orange (TO, Poncirus trifoliata) rootstock, which exhibits healthy green leaves, and the 'Newhall' navel orange (NHE, C. sinensis) grafted onto TO, which has typical magnesium (Mg) deficiency-indu...

    34. Citrus ACC synthase CiACS4 regulates plant height by inhibiting gibberellin biosynthesis

    文献信息:Chu, Le Le, Yan, Zhen, Sheng, Xing Xing, Liu, Hai Qiang, Wang, Qing Ye, Zeng, Ren Fang, Hu, Chun Gen and Zhang, Jin Zhi等.  请登录

    简介:Dwarfism is an agronomic trait that has substantial effects on crop yield, lodging resistance, planting density, and a high harvest index. Ethylene plays an important role in plant growth and development, including the determination of plant height. However, the mechanism by which ethylene regulates plant height, especially in woody plants, remains unclear. In this study, a 1-aminocyclopropane-1-c...

    35. Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina ci

    文献信息:de Oliveira Dorta, S., Borges Attilio, L., Zanuzo Zanardi, O., Spotti Lopes, J. R., Machado, M. A. and Freitas-Astua, J.等.  请登录

    简介:The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our grou...

    36. Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease

    文献信息:Docema, Matheus Luis, Moraes, Tatiana de Souza, Attilio, Lisia Borges, Rodrigues Marques, Joao Paulo, Liborio Stipp, Liliane Cristina, Harakava, Ricar等.  请登录

    简介: Currently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-t...

    37. A prophage-encoded effector from "Candidatus Liberibacter asiaticus" targets ASCORBATE PEROXIDASE6 in citrus to facilitate bacterial infection

    文献信息:Du, Jiao, Wang, Qiying, Shi, Hongwei, Zhou, Changyong, He, Jun and Wang, Xuefeng等.  请登录

    简介:Citrus huanglongbing (HLB), associated with the unculturable phloem-limited bacterium "Candidatus Liberibacter asiaticus" (CLas), is the most devastating disease in the citrus industry worldwide. However, the pathogenicity of CLas remains poorly understood. In this study, we show that AGH17488, a secreted protein encoded by the prophage region of the CLas genome, suppresses plant immunity via targ...

    38. Enhancing bioefficacy of Bacillus amyloliquefaciens SF14 with salicylic acid for the control of the postharvest citrus green mould

    文献信息:El Hamss, Hajar, Kajad, Nada, Belabess, Zineb and Lahlali, Rachid等.  请登录

    简介:Penicillium digitatum is a major post-harvest pathogen of citrus fruit causing tremendous economic losses. In this study, salicylic acid (SA) and antagonistic bacteria Bacillus amyloliquefaciens (SF14) were tested in controlling P. digitatum (green mould) infections as an alternative to chemical controls. Three concentrations of SA (0.5, 1, and 2%) were tested for their antifungal activity in both...

    39. Genome-wide genetic variation and comparative transcriptome analyses of citrus mutant Jedae-unshiu and wild-type Citrus unshiu

    文献信息:Eun, Chang-Ho and Kim, In-Jung等.  请登录

    简介:'Miyagawa-wase' mandarin (Citrus. unshiu Marc. cv. Miyagawa-wase) is one of the most widely cultivated citrus varieties in Korea. Previously, we used gamma-irradiation to develop new citrus varieties by mutation breeding, a useful tool for increasing genetic diversity in a short time. One of the new citrus varieties we generated, Jedae-unshiu, produces uniquely shaped fruit with vertical troughs o...

    40. The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit

    文献信息:Fang, Heting, Shi, Yanna, Liu, Shengchao, Jin, Rong, Sun, Jun, Grierson, Donald, Li, Shaojia and Chen, Kunsong等.  请登录

    简介:Sugars are fundamental to plant developmental processes. For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness. In citrus (Citrus reticulata Blanco.), we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents. Expression of a SuS (Sucrose Synthase) gene Cit...

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