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  • 161. Engineered Orange Ectopically Expressing the Arabidopsis β-Caryophyllene Synthase Is Not Attractive to Diaphorina citri, the Vector of the Bacterial Pathogen Associated to Huanglongbing

    文献信息:Berta Alquezar; Haroldo Xavier Linhares Volpe; Rodrigo Facchini Magnani; Marcelo Pedreira de Miranda; Mateus de Almeida Santos; Viviani Vieira Marques等.  请登录

    简介:Huanglongbing (HLB) is a destructive disease, associated with psyllid-transmitted phloem-restricted pathogenic bacteria, which is seriously endangering citriculture worldwide. It affects all citrus species and cultivars regardless of the rootstock used, and despite intensive research in the last decades, there is no effective cure to control either the bacterial species (Candidatus Liberibacter sp...

    162. Engineering of citrus to obtain huanglongbing resistance

    文献信息:Alquezar, Berta; Carmona, Lourdes; Bennici, Stefania; Pena, Leandro等.  请登录

    简介:Huanglongbing (HLB) disease is threatening the sustainability of citriculture in affected regions because of its rapid spread and the severity of the symptoms it induces. Herein, we summarise the main research findings that can be exploited to develop HLB-resistant cultivars. A major bottleneck has been the lack of a system for the ex vivo cultivation of HLB-associated bacteria (CLs) in true plant...

    163. Genome-wide identification and expression analysis of Polyamine Uptake Transporter gene family in sweet orange (Citrus sinensis)

    文献信息:Alhag, A.; Song, J.; Dahro, B.; Wu, H.; Khan, M.; Salih, H.; Liu, J. H.等.  请登录

    简介:Polyamine uptake transporter (PUT) plays important roles in polyamine homeostasis, but knowledge regarding PUT family genes in sweet orange (Citrus sinensis) remains elusive. Herein, our study aimed to perform a genome-wide identification of the PUT gene family in C. sinensis. A total of eight putative PUT genes (CsPUT1-CsPUT8) were identified in the sweet orange genome and distributed on three ch...

    164. Microspore Embryogenesis in Citrus

    文献信息:Yahyaoui; Emna; Germana; Maria Antonietta等.  请登录

    简介:This chapter deals with microspore embryogenesis in Citrus. Microspore embryogenesis allows to induce immature gametes (microspores) and to deviate them, in this case, the male one, from the normal gametophytic developmental route in the direction of the sporophytic one, yielding homozygous organisms (embryos and plants).

    165. CsLOB1 regulates susceptibility to citrus canker through promoting cell proliferation in citrus

    文献信息:Zou, X; Du, M; Liu, Y; Wu, L; Xu, L; Long, Q; Peng, A; He, Y; Andrade, M; Chen, S.等.  请登录

    简介:Citrus sinensis lateral organ boundary 1 (CsLOB1) was previously identified as a critical disease susceptibility gene for citrus bacterial canker, which is caused by Xanthomonas citri subsp. citri (Xcc). However, the molecular mechanisms of CsLOB1 in citrus response to Xcc are still elusive. Here, we constructed transgenic plants overexpressing and RNAi-silencing of CsLOB1 using the canker-disease...

    166. Overexpression of Salicylic Acid Carboxyl Methyltransferase (CsSAMT1) Enhances Tolerance to Huanglongbing Disease in Wanjincheng Orange (Citrus sinensis (L.) Osbeck)

    文献信息:Zou, Xiuping; Zhao, Ke; Liu, Yunuo; Du, Meixia; Zheng, Lin; Wang, Shuai; Xu, Lanzhen; Peng, Aihong; He, Yongrui; Long, Qin; Chen, Shanchun等.  请登录

    简介:Citrus Huanglongbing (HLB) disease or citrus greening is caused by Candidatus Liberibacter asiaticus (Las) and is the most devastating disease in the global citrus industry. Salicylic acid (SA) plays a central role in regulating plant defenses against pathogenic attack. SA methyltransferase (SAMT) modulates SA homeostasis by converting SA to methyl salicylate (MeSA). Here, we report on the functio...

    167. 冰糖橙新品种‘锦秀’的选育

    文献信息:张春苗; 胡成; 曾亮; 柴爽; 龙桂友; 孙太安; 李大志; 张玲; 邓子牛; 李娜等.  请登录

    简介:‘锦秀’是从普通冰糖橙芽变中选育出的冰糖橙新品种。果实扁圆,果面橙色,果面光滑,果肉浅橙色,果实平均纵径62.99 mm,横径72.33 mm,果形指数为1.08,单果质量188 g,果皮厚5.57 mm,每果实含种子0.2粒,出汁率64.52%,可溶性固形物含量(w,后同)为12.0%,总糖含量10.89%,可滴定酸含量0.2%,固酸比60,维生素C含量(ρ)为58.08 mg·100 mL~(-1),可食率为76.53%。果实生育期210 d,在湖南省麻阳县11月下旬成熟。抗寒、抗旱和抗病性与普通冰糖橙类...

    168. 甜橙CsHSFA2基因克隆及其耐寒性研究

    文献信息:张亦武等.  请登录

    简介:柑橘为世界第一大果树,分布广泛,江苏苏南一带也有种植,而低温胁迫严重制约了柑橘的产量与品质。热激转录因子HSFA2在逆境响应中充当正调控因子,是抗逆基因工程的理想候选基因。但HSFA2基因在柑橘抗寒中的作用尚不明确。因此,本研究拟克隆甜橙HSFA2基因及其启动子,分析其各种胁迫下表达情况,并通过转基因等手段对其抗寒功能进行初步探究。主要结果如下:1、根据柑橘基因组数据库(http://citrus.hzau.cn/orange/),从甜橙中扩增获得1155...

    169. Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures

    文献信息:Dutt, Manjul, Mou, Zhonglin, Zhang, Xudong, Tanwir, Sameena E. and Grosser, Jude W.等.  请登录

    简介:Background Development of precise genome editing strategies is a prerequisite for producing edited plants that can aid in the study of gene function and help understand the genetic traits in a cultivar. Citrus embryogenic cell cultures can be used to rapidly produce a large population of genome edited transformed citrus lines. The ability to introduce specific mutations in the genome of these cell...

    170. Knockout or Silencing of the NPR3 Homolog Enhances Pathogenesis-Related Gene 1 (PR1) Expression in Citrus

    文献信息:Dutt, Manjul, Tanwir, Sameena E., Mahmoud, Lamiaa M., Grosser, Jude W. and Mou, Zhonglin等.  请登录

    171. CsWAKL08, a pathogen-induced wall-associated receptor-like kinase in sweet orange, confers resistance to citrus bacterial canker via ROS control and JA signalin

    文献信息:Qiang Li, Anhua Hu, Jingjing Qi, Wanfu Dou, Xiujuan Qin, Xiuping Zou, Lanzhen Xu, Shanchun Chen等.  请登录

    172. CsPrx25, a class III peroxidase in Citrus sinensis, confers resistance to citrus bacterial canker through the maintenance of ROS homeostasis and cell wall lignification

    文献信息:Qiang Li, Xiujuan Qin, Jingjing Qi, Wanfu Dou, Christophe Dunand, Shanchun Chen, Yongrui He等.  请登录

    173. Expression of Hexokinase in Stomata of Citrus Fruit Reduces Fruit Transpiration and Affects Seed Development

    文献信息:Lugassi, N., Kelly, G., Arad, T., Farkash, C., Yaniv, Y., Yeselson, Y., Schaffer, A. A., Raveh, E., Granot, D. and Carmi, N.等.  请登录

    简介:The temporal formation and spatial distribution of stomata on the surface of citrus floral organs and, specifically, on the ovule from which the fruit develops, were analyzed using citrus plants that express green fluorescent protein (GFP) under the guard cell-specific KST1 promoter. Stomata are found on the style, sepal, and anther of the closed flower and on ovules from the stage of anthesis. It...

    174. The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions

    文献信息:Soares, J. M.; Weber, K. C.; Qiu, W.; Stanton, D.; Mahmoud, L. M.; Wu, H.; Huyck, P.; Zale, J.; Al Jasim, K.; Grosser, J. W.; Dutt, M.等.  请登录

    简介:Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo citrange rootstocks expressing either Citrus clementina FT1 or FT3 genes under the control of the Arabidopsis th...

    175. Optimizing Recovery of Hybrid Embryos from Interspecific Citrus Crosses of Polyembryonic Rough Lemon (Citrus jambhiri Lush.)

    文献信息:Singh, Jagveer; Dhaliwal, Harvinder Singh; Thakur, Anirudh; Sidhu, Gurupkar Singh; Chhuneja, Parveen; Gmitter, Frederick G.等.  请登录

    简介:The prevalence of rough lemon rootstock in India is presently threatened by the spread of Phytophthora root rot. Developing an alternative Phytophthora-resistant rootstock that also withstands other abiotic constraints is a priority for a sustainable citrus industry in India. Complementary progenitors can be found in citrus germplasm to combine the desired traits, particularly between Citrus and P...

    176. New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

    文献信息:Salonia, Fabrizio; Ciacciulli, Angelo; Poles, Lara; Pappalardo, Helena Domenica; La Malfa, Stefano; Licciardello, Concetta等.  请登录

    简介:New plant breeding techniques (NPBTs) aim to overcome traditional breeding limits for fruit tree species, in order to obtain new varieties with improved organoleptic traits and resistance to biotic and abiotic stress, and to maintain fruit quality achieved over centuries by (clonal) selection. Knowledge on the gene(s) controlling a specific trait is essential for the use of NPBTs, such as genome e...

    177. Expression of Hexokinase in Stomata of Citrus Fruit Reduces Fruit Transpiration and Affects Seed Development

    文献信息:Lugassi Nitsan; Kelly Gilor; Arad Tal; Farkash Chagai; Yaniv Yossi; Yeselson Yelena; Schaffer Arthur A; Raveh Eran; Granot David; Carmi Nir等.  请登录

    简介:The temporal formation and spatial distribution of stomata on the surface of citrus floral organs and, specifically, on the ovule from which the fruit develops, were analyzed using citrus plants that express green fluorescent protein (GFP) under the guard cell-specific KST1 promoter. Stomata are found on the style, sepal, and anther of the closed flower and on ovules from the stage of anthesis. It...

    178. Systematic Analysis and Functional Validation of Citrus Pectin Acetylesterases (CsPAEs) Reveals that CsPAE2 Negatively Regulates Citrus Bacterial Canker Development

    文献信息:Li, Qiang; Fu, Jia; Qin, Xiujuan; Yang, Wen; Qi, Jingjing; Li, Zhengguo; Chen, Shanchun; He, Yongrui等.  请登录

    简介:The present study was designed to serve as a comprehensive analysis of Citrus sinensis (C. sinensis) pectin acetylesterases (CsPAEs), and to assess the roles of these PAEs involved in the development of citrus bacterial canker (CBC) caused by Xanthomonas citri subsp. citri (Xcc) infection. A total of six CsPAEs were identified in the genome of C. sinensis, with these genes being unevenly distribut...

    179. Microsatellites assisted rapid identification of mandarin hybrids and assessment of their phenotypic variability

    文献信息:Kaur, Kirandeep; Kumar, Krishan; Kaur, Kamaljit; Arora, Parshotam Kumar; Singh, Kuldeep等.  请登录

    简介:Kinnow ( Citrus nobilis x C. deliciosa) is a commercially important mandarin but contains large number of seeds. Mukaku Kishu (C. kinokuni), a seedless mandarin can be used to transfer seedlessness in Kinnow through hybridization. But, the polyembryony in Kinnow and absence of any heritable morphological trait in Mukaku Kishu are limiting factors in early identification of hybrids. SSR markers due...

    180. Assembly of Satsuma mandarin mitochondrial genome and identification of cytoplasmic male sterility-specific ORFs in a somatic cybrid of pummelo

    文献信息:Zhang, Shuai; Yin, Zhao-Ping; Wu, Xiao-Meng; Li, Chao-Chao; Xie, Kai-Dong; Deng, Xiu-Xin; Grosser, Jude W.; Guo, Wen-Wu等.  请登录

    简介:Cytoplasmic male sterility (CMS) and seedlessness are highly desirable traits for citrus. However, a high-quality reference mitochondrial genome of citrus is unavailable, and the mechanisms of CMS remain to be elucidated in citrus. We previously generated a male sterile somatic cybrid of pummelo (G1+HBP) by fusing protoplasts from CMS 'Guoqing No. 1' Satsuma mandarin (G1) and fertile 'Hirado Bunta...

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