文献信息: 祁静静等. 请登录
简介:柑橘溃疡病是由地毯黄单胞杆菌柑橘致病变种(Xanthomonas citri subsp.Citri,Xcc)引起的细菌性病害,是危害全球柑橘产业的最严重病害之一。筛选抗病基因,培育抗病柑橘品种的分子育种已成为防治柑橘溃疡病的主要方法之一。本研究前期通过转录组数据结合溃疡病菌诱导柑橘抗、感品种的实时定量PCR(Quantitative reverse transcription polymerase chain reaction,q RT-PCR)结果筛选得到可能与柑橘溃疡病相关的CsLYK6基因,通过反向遗...
文献信息:崔璐璐, 党江波, 韩国辉, 郭启高, 向素琼, 梁国鲁等. 请登录
简介:多倍体植株具有少核、有效成分含量高、树形矮化、抗逆性强等特点,在大田作物和果树育种中均有应用。梨橙是我国自主选育的优质甜橙品种,种子单胚,也有少量多胚种子出现。至今未见关于梨橙多倍体及梨橙单胚种子杂种性的报道。本研究以梨橙为材料,开展多倍体发掘和遗传鉴定研究。流式细胞术筛选与染色体显微观察相结合,对其自然产生的单胚种子萌发植株进行筛选,获得了三倍体2株、四倍体1株;InDel标记分析证实这些多倍体均为有性胚产生...
文献信息:彭爱红等. 请登录
简介:柑橘黄龙病(Citrus Huanglongbing,HLB)是柑橘生产上最具毁灭性的细菌病害,已对全球的柑橘产业造成了巨大的经济损失。其主要致病菌为韧皮部杆菌属亚洲种Candidatus Liberibacter asiaticus(CLas)。目前在柑橘属植物中没有发现抗HLB的种质,也没有从感病植株中彻底根除黄龙病菌的有效方法。培育抗病品种是控制黄龙病最根本的方法,对柑橘产业的可持续健康发展有至关重要的作用。诱导植物产生系统获得抗性,以及在植物中表达对病原菌...
文献信息:Wang, Wenjun; Li, Ting; Chen, Qi; Deng, Bing; Deng, Lili; Zeng, Kaifang等. 请登录
简介:Penicillium digitatum is the primary pathogen that causes serious yield losses worldwide. In our previous study, CsWRKY transcription factors (TFs) and some genes associated with immunity were identified in citrus fruits after P. digitatum infection, but little information is available in the literature on the mechanisms of TFs in citrus disease resistance. In this study, the possible mechanisms o...
文献信息:Wang, Nian; Jia, Hongge等. 请登录
简介:The invention pertains to a plant cell or a plant having one or more mutations in the promoters of both the alleles for CsLOB1 gene, wherein the one or more mutations are in the promoter binding sites for PthA4 protein from Xanthomonas spp., and wherein the one or more mutations reduce or abolish the binding of the Xanthomonas spp. PthA4 protein on to the binding sites in the promoters of the CsLO...
文献信息:Wang, Lun; Huang, Yue; Liu, ZiAng; He, Jiaxian; Jiang, Xiaolin; He, Fa; Lu, Zhihao; Yang, Shuizhi; Chen, Peng; Yu, Huiwen; Zeng, Bin; Ke, Lingjun; Xie等. 请登录
简介:An improved reference genome of sweet orange and newly sequenced genomes of its somatic mutants uncover the global pattern of somatic variations, the diversification and dispersal history of sweet orange and candidate genes controlling fruit taste and flavour. Somatic variations are a major source of genetic diversification in asexual plants, and underpin clonal evolution and the breeding of asexu...
文献信息:Wang, Fu; Chen, Lin; Chen, Shiwei; Chen, Hongping; Liu, Youping等. 请登录
简介:Citrus cultivars are widely spread worldwide, and some of them only differ by specific mutations along the genome. It is difficult to distinguish them by traditional morphological identification. To accurately identify such similar cultivars, the subtle differences between them must be detected. In this study, UPLC-ESI-MS/MS-based widely targeted metabolomics analysis was conducted to study the ch...
文献信息:Tupke, A. H.; Akhare, A. A.; Gahukar, S. J.; Bahatkar, B. P.; Zadokar, A. R.; Gaikwad, P. N.; Katkade, R. R.等. 请登录
简介:In the present investigation, seven different species specific SCAR markers were used for discriminating Galgal rootstock from other citrus species under study. The PDKV-1, PDKV-2, PDKV-3, PDKV-4, PDKV-5, PDKV-6 and PDKV-7 show the band size of 283 bp, 159 bp, 172 bp, 347 bp, 150 bp, 137 bp and 347 bp, respectively. The PIC value ranged from 0.38 to 0.88. The highest PIC value was observed in PDKV...
文献信息:Tang, Xiaomei; Wang, Xia; Huang, Yue; Ma, Ling; Jiang, Xiaolin; Rao, Muhammad Junaid; Xu, Yuantao; Yin, Ping; Yuan, Meng; Deng, Xiuxin; Xu, Qiang等. 请登录
简介:Author summary It has been well documented that most citrus cultivars are susceptible to canker disease, while little is known about the resistance or susceptibility of primitive or wild citrus to canker disease. This study reveals that primitive citrus (Atalantia buxifolia) is highly resistant to citrus canker. Transcriptome data demonstrated that Atalantia had an active resistance response to th...
文献信息:Sun, Yong-Duo; Zhang, Lei; Folimonova, Svetlana Y.等. 请登录
简介:To defend against pathogens, plants have developed a complex immune system, which recognizes the pathogen effectors and mounts defence responses. In this study, the p33 protein of Citrus tristeza virus (CTV), a viral membrane-associated effector, was used as a molecular bait to explore virus interactions with host immunity. We discovered that Citrus macrophylla miraculin-like protein 2 (CmMLP2), a...
文献信息:Samarina, L. S.; Kulyan, R. V.; Koninskaya, N. G.; Gorshkov, V. M.; Ryndin, A. V.; Hanke, M. V.; Flachowsky, H.; Reim, S.等. 请登录
简介:Characterization of genetic diversity in germplasm collections is fundamental for an efficient management and utilization. The citrus germplasm in the Western Caucasus represent one of the most northerly citrus collections. The collection contains numerous cultivars specifically selected for frost-tolerance, which are valuable as genetic resources for citrus breeding programs worldwide. Currently,...
文献信息:Lourkisti Radia; Oustric Julie; Quilichini Yann; Froelicher Yann; Herbette Stéphane; Morillon Raphael; Berti Liliane; Santini Jérémie等. 请登录
简介:Polyploidy plays a major role in citrus plant breeding to improve the adaptation of polyploid rootstocks as well as scions to adverse conditions and to enhance agronomic characteristics. In Citrus breeding programs, triploidy could be a useful tool to react to environmental issues and consumer demands because the produced fruits are seedless. In this study, we compared the physiological, biochemic...
文献信息:Peng, A.; Zou, X.; He, Y.; Chen, S.; Liu, X.; Zhang, J.; Zhang, Q.; Xie, Z.; Long, J.; Zhao, X.等. 请登录
简介:KEY MESSAGE: Overexpression of CiNPR4 enhanced resistance of transgenic citrus plants to Huanglongbing by perceiving the salicylic acid and jasmonic acid signals and up-regulating the transcriptional activities of plant-pathogen interaction genes. Developing transgenic citrus plants with enhanced immunity is an efficient strategy to control citrus Huanglongbing (HLB). Here, a nonexpressor of patho...
文献信息:Orbovic, Vladimir; Ravanfar, Seyed Ali; Acanda, Yosvanis; Narvaez, Javier; Merritt, Benjamin A.; Levy, Amit; Lovatt, Carol J.等. 请登录
简介:Transgenic 'Duncan' grapefruit (Citrus paradisi Macf.) and 'Valencia' sweet orange (Citrus sinensis [L.] Osbeck) plants ectopically expressing C. sinensis (cv. Washington navel orange) APETALA1 (CsAP1) or LEAFY (CsLFY) genes under control of the Arabidopsis thaliana stress-inducible promoter AtRD29A flowered under non-inductive (warm temperature, well-watered) greenhouse conditions, whereas their ...
文献信息:Moniruzzaman, M.; Zhong, Yun; Huang, Zhifeng; Yan, Huaxue; Yuanda, Lv; Jiang, Bo; Zhong, Guangyan等. 请登录
简介:Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this stud...
文献信息:Mitre, L. K.; Teixeira-Silva, N. S.; Rybak, K.; Magalhaes, D. M.; de Souza-Neto, R. R.; Robatzek, S.; Zipfel, C.; de Souza, A. A.等. 请登录
文献信息:Mercuri, Frank; Mercuri, Domenic; Nardi, Frank; Nardi, Michael; Nardi, Joe等. 请登录
简介:A new orange tree named 'Villa11' characterized by its late-maturing fruit.
文献信息:Lu, Suwen; Ye, Junli; Zhu, Kaijie; Zhang, Yin; Zhang, Mengwei; Xu, Qiang; Deng, Xiuxin等. 请登录
简介:Carotenoids in citrus contribute to the quality of the fruit, but the mechanism of its transcriptional regulation is fairly unknown. Here, we characterized a citrus FRUITFULL sub-clade MADS gene, CsMADS5, that was ripening-inducible and acted as a nucleus-localized trans-activator. Transient overexpression of CsMADS5 in citrus induced fruit coloration and enhanced carotenoid concentrations. The ex...
文献信息:Louzada, Eliezer S.; Ramadugu, Chandrika等. 请登录
简介:Grapefruit [Citrus xaurantium (synonym C. xparadisi)] is an important citrus commodity that originated in Barbados in the 17th century. Grapefruit is the youngest member of the genus Citrus. Most commercially important grapefruit cultivars arose through natural and induced mutations, not traditional breeding, of the white-fleshed and seedy Duncan grapefruit. Now, cultivars with a range of flesh co...
文献信息:Long, Q.; Du, M.; Long, J.; Xie, Y.; Zhang, J.; Xu, L.; He, Y.; Li, Q.; Chen, S.; Zou, X.等. 请登录
简介:Pathological hypertrophy (cell enlargement) plays an important role in the development of citrus canker, but its regulators are largely unknown. Although WRKY22 is known to be involved in pathogen-triggered immunity and positively regulates resistance to bacterial pathogens in Arabidopsis, rice and pepper, the CRISPR/Cas9-mediated partial knockout of CsWRKY22 improves resistance to Xanthomonas cit...