文献信息: Jin, S. B., Lee, D. H., Park, S. M., Park, J. S. and Moon, Y. E.等. 请登录
简介:Citrus peels are rich in antioxidants, such as carotenoids. The genus Fortunella, including varieties, such as 'Meiwa,' allows for whole-fruit consumption, securing important genetic resources for producing high-quality and seedless citrus fruits by breeding and developing intergeneric fusion plants through protoplast fusion. Therefore, somatic cell fusion was performed between calli capable of so...
文献信息:Hu, Y., Li, F., Lu, N., Bao, K., Li, T., Zuo, X., Li, R., Wang, N. and Huang, G.等. 请登录
简介:Citrus Huanglongbing (HLB), caused by "Candidatus Liberibacter asiaticus" (CLas), is a devastating citrus disease worldwide, and its pathogenesis remains poorly understood. Here, we investigated the virulence function of a Sec-dependent effector, metal-dependent hydrolase 1 (MDH1), which is significantly upregulated in citrus during CLas infection compared to in psyllids. Overexpression of MDH1 in...
文献信息:Jin, L. F., Yue, Y., Liu, F., Wen, M. X., Huang, B. and Wang, P.等. 请登录
简介:Citric acid serves as the principal organic acid in citrus fruits, with its concentration critically determining fruit flavor and market acceptability. Isocitrate dehydrogenase (IDH), a key enzyme in citric acid metabolism, mediates the conversion of citrate to alpha-ketoglutarate. This study cloned six candidate genes encoding IDH from grapefruit (Citrus paradisi). Bioinformatics analysis showed ...
文献信息:He, X., Wang, H., Wei, W., Han, Z., Zuo, J. and He, Q.等. 请登录
简介:The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by 'Candidatus Liberibacter asiaticus' (CaLas). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to CaLas infection induction were screened, and one gene cloned with higher differential expression level was selected and named CsCESA1. we verified ...
文献信息:He, J. J., Hu, G., Shen, M. Y., Fan, Y. J., Shi, X. S., Wu, X. M., Guo, W. W., Xia, Q. M. and Xie, K. D.等. 请登录
简介:Interploidy hybridization between diploid and tetraploid has been an important approach to develop triploid hybrids in horticultural plants including citrus for seedless breeding. However, dysregulation of imprinted genes can lead to the failure of endosperm cellularization, resulting in abortion of triploid embryos before seed maturity, thereby impeding the efficiency of triploid generation. The ...
文献信息: He, Q. and He, X.等. 请登录
简介: Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to citrus disease-resistant breeding. Through the analysis of comparative transcriptome data, we identified six cell wall degradation genes that respond to citrus infection with CaLas. We selected one of the genes with high differential expression levels and cloned it, naming it CsPG23. The subcellular local...
文献信息: Guo, X. R., Su, J., Xue, H., Sun, Y. J., Lian, M. Y., Ma, J. J., Lei, T. G., He, Y. R., Li, Q., Chen, S. C. and Yao, L. X.等. 请登录
简介: Huanglongbing (HLB) and citrus canker are two major destructive bacterial diseases in the citrus industry caused by Candidatus Liberibacter asiaticus (CLas) and Xanthomonas citri subsp. citri (Xcc), respectively. ABI5 transcription factors are crucial for plant growth and development as well as for responses to various abiotic and biotic stresses, including viruses and fungi. This study aimed to ...
文献信息:Glusberger, Paula Rios, Merritt, Benjamin, Liu, Cheng, Wang, Yu, Zale, Janice, Wu, Hao, Canton, Michel, Braverman, Michael, Triplett, Eric W. and Mou,等. 请登录
简介:Citrus greening disease, or Huanglongbing (HLB), has caused devastating losses to citrus production in Florida, with yields declining by over 90% since 2005. Despite extensive efforts, no sustainable solution has been widely effective. Here, transgenic 'Hamlin' sweet orange lines engineered to constitutively express the Arabidopsis NPR1 (AtNPR1) gene, a key regulator of systemic acquired resistanc...
文献信息:Fan, Y. J., Du, Z. Z., He, X. Y., Liu, Z. A., Zhuang, J. X., Xiao, G. A., Duan, Y. Y., Tan, F. Q., Xie, K. D., Jiao, W. B., Zhang, F., Yang, C., Guo, 等. 请登录
简介:Seedlessness is a most valuable trait in fruit crops for fresh consumption and processing. The mutations in essential meiosis genes are known to confer sterility and seed abortion in plants. However, defects in meiosis have rarely been reported in fruit crops. Here, we found meiosis defects caused sterility in a seedless citrus bud sport cultivar, with massive unpaired univalents during diakinesis...
文献信息:Fan, Y. J., Tan, F. Q., He, X. Y., Liu, Z. A., Bai, H. Z., Jiang, N., Jia, H. H., Yang, W. H., Song, X., Zhang, M., Xie, K. D., Guo, W. W. and Wu, X. 等. 请登录
简介:Male sterility (MS) holds great potential and value for breeding seedless cultivars of fruit trees. However, functional characterization of MS-related genes has been difficult in perennial fruit crops, because of the extended juvenile period and difficulties in genetic transformation. In this study, we compared anther development of 'Qianyang Seedless' (QS), a natural seedless bud sport (somatic m...
文献信息:Ezra, D., Carmi, N., Wang, X. L., Zhang, M. M., Li, X. Y., Zheng, S. H., Wang, F. S., Zhu, S. P. and Zhao, X. C.等. 请登录
简介:New plant breeding techniques are revolutionizing citrus improvement by accelerating trait enhancement and genetic gains. In recent years, technological advances have enabled more precise and accelerated breeding. This review discusses the state-of-the-art breeding technologies for citrus, including marker-assisted selection, genomic selection, genome editing (particularly CRISPR/Cas), somatic hyb...
文献信息:Docema, M. L., Moraes, T. D., Attilio, L. B., Piedade, S. M. D., Marques, J. P. R., Stipp, L. C. L., Harakava, R., Freitas-Astua, J. and Mourao, F. D.等. 请登录
简介:We evaluated the response of 'Hamlin' sweet orange plants (Citrus x sinensis L. Osbeck) transformed with the gene encoding the synthetic antimicrobial peptide d4e1, driven by the 35S promoter, or with the gene encoding copper-zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata and driven by the Ubiquitin 10 promoter, against Candidatus Liberibacter asiaticus (CLas). The experimenta...
文献信息:Deol, J. K., Ramekar, S. and Dutt, M.等. 请登录
简介: Genotyping by Sequencing (GBS) is an innovative technology that evaluates genotypes based on their nucleotide sequences, aiding in the identification of promising cultivars with desirable traits that can greatly influence plant breeding. GBS examines genetic variation at the sequence level, making it a valuable tool for addressing challenges in plant breeding. It has been widely used to study res...
文献信息:de Souza-Neto, R. R., Cavalcante, L. N., Carvalho, I. G. B., Curtolo, M., Benedetti, C. E., Takita, M. A., Wang, N. and de Souza, A. A.等. 请登录
简介:The Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. citri effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance to citrus canker disease. Previous studies revealed that the citrus CsLOB1-INDUCED EXPANSIN 1 gene (CsLIEXP1) is highl...
文献信息:Darolt, J. C., Raiol, L. L., Jr., Carvalho, E. V., Andrade, L. L., Oliveira, V. S. L., Gomes, P. A., Alves, M. N., Moreira, A. S., Volpe, H. X. L., Ci等. 请登录
简介:Genetic resistance to 'Candidatus Liberibacter asiaticus' (CLas) is considered a promising long-term solution to control Huanglongbing (HLB), given that all commercial citrus varieties are susceptible to the disease. Only a few citrus relatives have been reported as putatively CLas resistant; however, these were only evaluated as scion varieties. Here, we assessed CLas-induced damages and titer in...
文献信息: Cui, X. J., Ren, L., Yuan, Y. Z., Wang, Y. L., Li, X. Y., Ma, S. M., Fu, S. M., Zhou, C. Y. and Wang, X. F.等. 请登录
简介:Autophagy, a cellular process involved in the degradation and recycling of cellular components, has emerged as a pivotal mechanism for maintaining cellular homeostasis and combating pathogen invasion. Here, we provide evidence that the overexpression of CsATG8c inhibits CLas proliferation in citrus. CsATG8c directly interacts with the CLas effector protein SDE4040, leading to its degradation via t...
文献信息:Culver, J. N., Vallar, M., Burchard, E., Kamens, S., Lair, S., Qi, Y. P., Collum, T. D., Dardick, C., El-Mohtar, C. A. and Rogers, E. E.等. 请登录
简介: BackgroundThe analysis of translationally active mRNAs, or translatome, is a useful approach for monitoring cellular and plant physiological responses. One such method is the translating ribosome affinity purification (TRAP) system, which utilizes tagged ribosomal proteins to isolate ribosome-associated transcripts. This approach enables spatial and temporal gene expression analysis by driving th...
文献信息:Cui, X. J., Du, M. X., Zheng, X. Y., Ren, L., Yuan, Y. Z., Ma, S. M., Liu, C. F., Zou, X. P., Fu, S. M., Zhou, C. Y. and Wang, X. F等. 请登录
简介:Macroautophagy/autophagy functions in plant host immunity responses to pathogen infection. The molecular mechanisms and functions used by the citrus Huanglongbing (HLB)-associated intracellular bacterium "Candidatus Liberibacter asiaticus" (CLas) to manipulate autophagy are largely unknown. Here, we identified a CLas effector, SDE4580 (CLIBASIA_04580), which contributes to HLB progression. Transge...
文献信息:Chen, X. H., Fang, F., Chen, T. T., Wu, J. H., Zheng, Z. and Deng, X. L.等. 请登录
简介:Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), is the most devastating disease threatening global citrus production. Although no commercial citrus varieties exhibit complete HLB resistance, genotype-specific tolerance variations remain underexplored. This study conducted a comparative transcriptomic profiling of six commercially citrus cultivars in South China, four susce...
文献信息:Chen, M., Zhang, T. L., Zhang, W. B., Wen, Y. Z., Ma, Z. X., Xi, Z. P., Hu, C. G. and Zhang, J. Z.等. 请登录
简介:Plant height and optimal flowering time are key determinants of crop yield and economic value. However, the regulatory mechanisms governing these traits, particularly in woody plants, remain unclear. In this study, overexpression of a citrus microRNA156 (miR156) family member, Ci-miR156c, resulted in significant phenotypic changes in citrus, including reduced plant height and delayed flowering. mi...