文献信息:McCue, K. F., Shao, M., Huynh, J., Minh, T., Chan, M. Y., Moy, Y. and Thomson, J. G.等. 请登录
简介: Efficient selection of transgenic plants is essential in plant biotechnology, and development of effective selectable marker systems plays a crucial role, especially as multiple rounds of transformation and gene stacking may require multiple selectable markers. The present study aims to develop and evaluate an enhanced spectinomycin resistance construct to provide robust selection for transformat...
文献信息:Mei, Y. L., Shen, P., Yang, Z. Y., Fu, S. M., Shi, Y. Q., Cui, X. J., Wang, J. J., Zhang, S. S., Zhou, C. Y. and Wang, X. F.等. 请登录
简介: Ubiquitin-proteasome is a conserved mechanism that regulates cellular responses and disease resistance in plants. However, the regulation role of ubiquitin-proteasome in the pathogenicity of "Candidatus Liberibacter asiaticus" (CLas), the causal agent of citrus Huanglongbing, one of the most serious citrus diseases, remains poorly defined. In this study, we identified a CLas effector, SDE5640 (CL...
文献信息:Mao, Z. L., Li, M. D., Gao, S. Y., Zhao, Z. Q., Zhai, X. W., Liu, J. H., Cheng, L. L. and Li, C. L.等. 请登录
简介:Abscisic acid (ABA) and calcium respectively work as crucial plant hormones and second signalling molecules in the regulation of fruit development and quality formation, including the sugar content and flavour quality. However, the regulatory mechanisms of fruit sugar accumulation arising from the interaction between ABA and calcium have not yet been fully elucidated. Here, we show that the applic...
文献信息:Mahmoud, L. M., Jones, S. E., Gonzalez-Blanco, P., Fahong, Y., Dutt, M. and Killiny, N.等. 请登录
简介:Background'Parson Brown' sweet orange is an early-maturing variety and is considered a resilient tree in the face of Huanglongbing (Citrus Greening) disease. Its ability to maintain productivity under endemic HLB conditions has demonstrated its value for growers battling this devastating disease. This study compared the metabolomic profile, transcriptomic analysis, and physiological responses of t...
文献信息:Mahmoud, L. M. and Dutt, M.等. 请登录
简介:Citrus greening disease, or Huanglongbing (HLB), is the most destructive disease affecting citrus crops worldwide. All commercially cultivated citrus varieties are highly susceptible to HLB, and currently, no effective treatments exist. Several Australian lime species have demonstrated significant HLB tolerance, making them promising candidates for developing HLB-tolerant rootstocks and scions thr...
文献信息:Mahmoud, L. M., Deol, J. K., Grosser, J. W., Killiny, N. and Dutt, M.等. 请登录
简介: BackgroundHuanglongbing (HLB) is a devastating bacterial disease caused by the bacterium Candidatus Liberibacter asiaticus (CaLas) that affects the citrus industry worldwide. This study investigated the response of two pummelo x finger lime hybrid siblings to natural infection with CaLas. The hybrids were identified primarily using leaf morphology and molecular marker assessments and were selecte...
文献信息: Ma, Juanjuan, Jiang, Renhao, Tao, Xiaolong, Guo, Xingru, Xu, Lanzhen and Zou, Xiuping等. 请登录
简介: Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), represents a significant threat to the global citrus industry. LATERAL ORGAN BOUNDARIES 1 (LOB1) has been identified as a key gene involved in the development of citrus canker in susceptible varieties. However, the role and molecular mechanisms of LOB1 in disease-resistant varieties, such as Kumquat (Fortunella spp.), remain poorly un...
文献信息:Liu, X. Y., Chen, B. B., Luo, L., Zhong, Q., Teo, C. H. and Huang, S. J.等. 请登录
简介:Flavonoid synthases (FNSs) are key enzymes catalyzing the conversion of flavanones to flavonoids, yet their functions in citrus remain functionally uncharacterized. In this study, we identified three FNSII genes in the citrus genome. Phylogenetic analysis revealed that citrus FNSII genes share the closest evolutionary distance with apple FNSII genes. Chromosomal localization demonstrated that the ...
文献信息: Luro, F., Marchi, E., Costantino, G., Paoli, M. and Tomi, F.等. 请登录
简介:Pummelo (Citrus maxima) is an ancestral species that has given rise to several major citrus varieties, such as sweet orange (C. x aurantium var. sinensis) and grapefruit (C. x aurantium var. paradisi). This species is also cultivated and its fruit consumed, particularly in Asia. Over the course of evolution, the allogamous reproduction of pummelos and the absence of asexual multiplication have con...
文献信息:Liu, F. J., Long, S. Y., Hu, J. H., Qiao, X. H., Chen, L., Zhou, Y. and Zou, X. P.等. 请登录
简介:Citrus target spot disease poses significant threats to the citrus industry. However, the molecular mechanisms underlying citrus resistance to Pseudofabraea citricarpa remain unexplored. In this study, we identified the LBD family gene CsLOB1 and investigated its molecular responses to pathogen infection. Following P. citricarpa inoculation, exogenous methyl jasmonate (MeJA) and ethylene (ET), CsL...
文献信息:Liu, S. J., Bei, X. J., Li, Y. W., Gao, X., Wang, F. S., An, X. Y., Wu, Q. J., Huang, J. M., Lu, L. X., Liu, H. M., Long, C. R., Xu, Y. T., Wang, X., 等. 请登录
简介:Pummelo (Citrus maxima) is a fundamental species of Citrus which contributes to most of the cultivated citrus, including sweet orange, lemon and etc. The fruit neck is a structural feature of pummelo, and a long fruit neck reduces the edible rate of the fruit. In this study, we assembled a telomere-to-telomere (T2T) gap-free reference genome for the typical short fruit neck cultivar, 'Pingshan' pu...
文献信息:Li, Y. J., Hu, W., Ganie, S. A., Liu, Z. R., Cheng, B. P., Duan, H. and Li, Y.等. 请登录
简介:Agrobacterium-mediated transient gene expression is a powerful technique for rapidly evaluating gene expression in higher plants. This study aimed to improve transient expression levels of T-DNA genes in citrus by investigating the effects of various factors, including seedling age, tissue treatments, Agrobacterium incubation medium and duration, hormone combinations, methylation inhibitors, antio...
文献信息: Liao, P., Dan, X., Ge, W., Zhang, Q., Zhao, J. F., Zhou, C. Y. and Zhou, Y.等. 请登录
简介: Glycerophosphodiester phosphodiesterase 1 (GDPD1) plays an important function in the abiotic stress responses and participates in the accumulation of sn-glycerol-3-phosphate (G3P) in plants, which is key to plant systemic acquired resistance (SAR). However, the role of GDPD1 in plant responses to biotic stress remains poorly understood. This study characterized the antivirus function of the GDPD1...
文献信息:Li, Y., Lou, H. J., Fu, H. Y., Su, H. Y., Hao, C. X., Luo, J. M., Cai, N., Jin, Y., Han, J., Deng, Z. N., Cao, Y. L. and Ma, X. F.等. 请登录
简介:Citrus canker is a devastating disease caused by Xanthomonas citri subsp. citri (Xcc), which secretes the effector PthA4 into host plants to trigger transcription of the susceptibility gene CsLOB1, resulting in pustule formation. However, the molecular mechanism underlying CsLOB1-mediated susceptibility to Xcc remains elusive. This study identified CsCEL20 as a target gene positively regulated by ...
文献信息: Li, Y., Liu, Z., Gmitter, F. G., Jr., Deng, Z., Cheng, B., Duan, H. and Li, Y.等. 请登录
简介: Citrus production is threatened by biotic and abiotic stresses, particularly Huanglongbing (HLB), creating an urgent need for efficient engineering of citrus for disease resistance. Gene editing, especially transgene-free approaches, offers a promising alternative to traditional breeding, which is slow and constrained by citrus' long juvenile phase. However, producing transgene-free, genome-edite...
文献信息:Killiny, N., Kishk, A., Mahmoud, L. M., Dutt, M. and Stelinski, L. L.等. 请登录
简介:BACKGROUNDCitrus greening, or Huanglongbing (HLB), one of the most devastating citrus diseases worldwide, is caused by the phloem-limited bacterium 'Candidatus Liberibacter asiaticus' and transmitted by the Asian citrus psyllid (Diaphorina citri). Current disease control strategies rely primarily on insecticide applications to suppress vector populations, leading to environmental concerns and limi...
文献信息:Kelly, Gilor, Plesser, Elena, Bdolach, Eyal, Arroyave, Maria, Belausov, Eduard, Doron‐\Faigenboim, Adi, Rozen, Ada, Zemach, Hanita, Zach, Yair Yehoshu等. 请登录
简介:Recent advances in genome editing offer transformative potential for fruit tree breeding, yet conventional methods relying on tissue culture remain laborious and prone to somaclonal variation. Here, we present *in planta* genome editing in citrus (IPGEC), a novel Agrobacterium-mediated system enabling direct genome editing in soil-grown seedlings without tissue culture. IPGEC integrates transient ...
文献信息:Khadgi, A., Zayed, O., Sagawa, C. H. D., Zhang, F., Seymour, D. K. and Irish, V. F.等. 请登录
简介:Bacterial diseases like huanglongbing (HLB) and citrus canker severely impact citrus production. HLB, caused by "Candidatus Liberibacter asiaticus" (CLas), leads to tree decline, while citrus canker, caused by Xanthomonas citri pv. citri (Xcc) causes necrotic lesions on leaves and fruit. Many bacterial pathogens secrete effector proteins that suppress host plant immunity and promote pathogenesis t...
文献信息:Kelly, G., Plesser, E., Bdolach, E., Arroyave, M., Belausov, E., Doron-Faigenboim, A., Rozen, A., Zemach, H., Zach, Y. Y., Goldenberg, L., Arad, T., Y等. 请登录
简介: Recent advances in the field of genome editing offer a promising avenue for targeted trait improvements in fruit trees. However, the predominant method taken for genome editing in citrus (and other fruit trees) involves the time-consuming tissue culture approach, thereby prolonging the overall citrus breeding process and subjecting it to the drawbacks associated with somaclonal variation. In this...
文献信息:Jin, S. B., Park, S. M., Park, J. S., Moon, Y. E. and Lee, D. H.等. 请登录
简介:'Minihyang', a novel citrus cultivar, was developed through the hybridization of Citrus kinokuni ex Tanaka 'Kishu mikan' and Citrus reticulata 'Ootaponkan'. This study aimed to establish a tissue culture system for 'Minihyang' using cells from unfertilized and undeveloped seeds of mature fruits. Somatic embryogenic cells were induced on somatic embryogenesis induction modified Murashige and Tucker...