文献信息：Chen, A. Y. S., Spigolon, G., Scipioni, L. and Ng, J. C. K.等.  请登录

简介：The emergence of nanobodies (Nbs) has kindled an avid interest for their use in genetic engineering and plant biotechnology. In planta expression of Nbs has relied on either stable or transient transformation approaches that are lengthy and cannot support systemic expression, respectively. In addition, there is no precedence for studies on tissue-specific expression of Nbs. To address these issues...

文献信息：张成磊, 夏泽文, 徐鑫严, 刘高平, 潘丽芹, 伍小萌, 郭文武 and 解凯东等.  请登录

简介：【目的】本地早橘是浙江“黄岩蜜橘”区域品牌的代表品种，但由于果实有核和品质下降，其在黄岩乃至浙江栽培面积大幅下降。针对上述问题，采用细胞工程高效育种技术创制或发掘本地早橘多倍体无核/少核新种质，为“黄岩蜜橘”品牌健康发展提供优质资源储备。【方法】基于实生幼苗形态初选，发掘本地早橘疑似四倍体新种质；以本地早橘为母本与四倍体为父本倍性杂交，利用幼胚离体挽救培养创制本地早橘三倍体新种质；用流式细胞仪结合根...

文献信息：徐天予, 张迟, 王刚, 叶潇玲, 陈翔, 叶生月, 张小琴 and 张敏等.  请登录

简介：【目的】柑橘三倍体具有无核或少核的特点，通过4x×2x杂交方式创制三倍体种质，以弥补鸡尾葡萄柚种子多、抗寒性差的缺点。【方法】以四倍体（双二倍体）胡柚为母本与二倍体鸡尾葡萄柚杂交，果实成熟后对种子大小进行分类并进行组织培养，采用流式细胞仪、SSR分子标记鉴定再生子代的倍性及遗传组成。【结果】共授粉40朵花，坐果19个，坐果率为47.5%。获得的230粒种子中，大种子占56粒，再生49株，2株为三倍体（占大种子再生后代的4....

文献信息：宋放, 羊林鑫, 王策, 蒋迎春, 何利刚, 王志静, 马小方, 张豫, 宋鑫 and 吴黎明等.  请登录

简介：【目的】利用分子标记检测技术进行杂种鉴定和单多胚性检测，为柑橘新品种选育提供种质资源。【方法】利用分子标记技术对130株清见×鄂柑一号的杂交后代进行鉴定和亲缘关系分析，并结合形态学观察对杂交后代进行单多胚鉴定。【结果】引物CHR2-2、CHR8-3和CHR8-4能够对99.23%的杂种后代进行鉴定。通过聚类分析，大多数杂交后代与母本清见的亲缘关系比父本鄂柑一号亲缘关系近。通过对130株清见×鄂柑一号杂交后代进行单多胚鉴定分析，...

文献信息：黄伟, 柴爽, 张犇, 龚玲, 王双友, 李大志, 龙桂友 and 李娜等.  请登录

简介：【目的】利用重测序数据和表型鉴定信息解析宽皮柑橘种质资源遗传变异和群体结构情况，挖掘优异种质资源，促进品种改良朝优质多样化方向发展。【方法】2021、2022年连续2年对473份宽皮柑橘种质果实与花的25个表型进行鉴定，采其嫩叶进行DNA提取并建库测序。利用变异系数和Shannon-Wiener指数对表型数据进行遗传多样性分析，结合相关性分析、聚类分析、主成分分析等方法对宽皮柑橘种质资源进行综合评价，筛选优异宽皮柑橘种质。基于重...

文献信息：胡宇, 曹宗洪, 孙怡, 刘聪, 文豪, 刘慧敏, 郑鑫, 徐梦梦, 蒋祥东, 张友和, 李光浩, 叶俊丽, 邓秀新, 谢宗周 and 柴利军等.  发表于【https://link.cnki.net/doi/10.16420/j.issn.0513-353x.2024-0246】 | 请登录

简介：利用形态学和细胞学观察等手段探究了‘贡水白柚’的无核成因。结果表明，‘贡水白柚’雌、雄配子发育正常且可育；自交授粉后，花粉管生长停止在柱头1/3处，表现为不亲和；未授粉花朵去雄套袋后，7、14、21和49 d的坐果率分别为60%、32%、14%与9%，可发育成无核果实，表明其具有单性结实能力。此外，对‘贡水白柚’前期胚珠组织发育进行切片观察，发现其去雄后21d胚囊组织开始萎缩。其与‘沙田柚’和‘琯溪蜜柚’相互杂交表现为亲和...

文献信息：何礼, 张国艳, 李文婷, 关斌, 陈克玲, 刘建军 and 何建等.  发表于【https://link.cnki.net/doi/10.16213/j.cnki.scjas.2025.1.005】 | 请登录

简介：【目的】创制甜柠檬(Citrus lumia Risso)多倍体种质，创建其分子和细胞遗传组成鉴定新体系，为种质资源创新和鉴定利用提供新方法体系。【方法】通过实生苗根尖染色体计数筛选甜柠檬多倍体材料，与二倍体对比分析多倍体的叶形、气孔等植物学性状。利用47对分布于柑橘9条染色体上的SSR标记，分析多倍体甜柠檬的亲本来源。利用柑橘全部9条染色体特异的寡核苷酸荧光原位杂交(Oligo-FISH)涂染探针，精准鉴定多倍体甜柠檬的染色体组成。【...

文献信息：程瀚远, 郑杰荣, 徐宸宇, 伊华林 and 吴巨勋等.  发表于【 https://link.cnki.net/doi/10.13925/j.cnki.gsxb.20240334】 | 请登录

简介：【目的】探讨以HB柚为母本、华柑4号为父本的杂交后代群体果实相关性状的遗传规律，为杂交育种亲本的选配提供理论依据。【方法】测定该组合53个杂交后代果实的单果质量、纵径、横径、果皮厚度及维生素C、可滴定酸、可溶性固形物含量等性状指标，分析杂交后代的遗传规律。【结果】HB柚×华柑4号组合后代果实形状出现了4种类型，以高扁圆形、圆形或近圆形、椭圆形为主，比例分别为41.51%、39.62%和13.21%；果皮颜色在杂交群体后代中出...

文献信息：Zhao, M. M., Ye, R. C., Li, Y., Liu, L., Su, H. Y., Ma, X. F. and Deng, Z. N.等.  请登录

简介：Citrus bacterial canker disease, caused by Xanthomonas citri subsp. citri (Xcc), poses a significant global threat to the citrus industry. Lateral organ boundaries 1 (Lob1) is confirmed as a citrus susceptibility gene that induces pathogenesis by interaction with the PthA4 effector of Xcc. Citron C-05 (Citrus medica) is a Citrus genotype resistant to Xcc. However, there is little information avail...

文献信息：Zhang, F., Wang, Q. G., Luo, J. M., Gentile, A., Long, G. Y., Deng, Z. N., Li, D. Z. and Li, N.等.  请登录

简介：Seedless is a highly valued commercial characteristic in the citrus industry, both for fresh consumption and for processed markets. In this study, the 'Succari Sweet Orange' (WT) and its seedless mutant 'Juxiangyuan' (MT), which originated from a bud mutation, were selected to study the formation of a citrus seedless phenotype. Microscopic analysis of MT's floral organs, including anther and ovary...

文献信息：Xu, Y. H., Tian, W. X., Yin, M. Q., Cai, Z. M., Zhang, L., Yuan, D. Y., Yi, H. L. and Wu, J. X.等.  请登录

简介：Achieving seedlessness in citrus varieties is one of the important objectives of citrus breeding. Male sterility associated with abnormal pollen development is an important factor in seedlessness. However, our understanding of the regulatory mechanism underlying the seedlessness phenotype in citrus is still limited. Here, we determined that the miR159a-DUO1 module played an important role in regul...

文献信息：Yamamoto, M., Arita, S. and Kozai, N.等.  请登录

简介： Simple and easy methods for producing autotetraploids in polyembryonic citrus were developed. These methods require only colchicine treatment at the time of sowing and do not require special skills or equipment, such as in vitro culture. The ploidy of all growing plants was analyzed by flow cytometry. The percentage of tetraploid induction per seed in five accessions at optimal treatment was 11.0...

文献信息：Wang, R., Shi, Y. C., Xie, K. D., Xie, Z. Z., Wu, X. M. and Guo, W. W.等.  请登录

简介：To breed seedless varieties, cytoplasmic male sterile (CMS) mitochondria from the Satsuma mandarin Guoqing No. 1 (Citrus unshiu Marc. cv. G1) have been transferred to elite seedy cultivars via somatic hybridization. However, the question remains: can the CMS mitochondria from G1 effectively induce male sterility in diverse cultivated citrus species, such as pummelo, sweet orange, and mandarin, and...

文献信息：Wang, S., Zhao, S. L., Du, M. X., Mo, K. Q., Pang, S. W. and Zou, X. P.等.  请登录

简介：Conventional breeding techniques have been effectively utilized for the enhancement of citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross- or self-incompatibility, high genetic heterozygosity, and polyembryony have posed significant challenges and limitations to these methods. The clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has pro...

文献信息：Wang, Jiajun, Yu, Ling, Zhao, Jinfa, Fu, Shimin, Mei, Yalin, Lou, Binghai and Zhou, Yan等.  请登录

简介：Autophagy, one of the most widespread and highly conserved protein degradation systems in eukaryotic cells, plays an important role in plant growth, development and stress response. Beclin 1 is a core component of the phosphatidylinositol 3-kinase (PI3K) autophagy complex and positively regulates plant immunity against viruses. The upregulation of Eureka lemon ClBeclin1 was observed in response to...

文献信息：Wan, S. G., Liang, B. B., Yang, L., Hu, W., Kuang, L. Q., Song, J., Xie, J. H., Huang, Y. J., Liu, D. C. and Liu, Y.等.  请登录

简介：Citrus quality and yield are severely declined under drought stress. To date, the effects of MADS-box family transcription factors (TFs) on plant drought resistance have made some progress. However, whether MADS-box family TFs are associated with citrus drought response has remained unclear. The current paper identified a MADS-box family gene PtrANR1 encoding anthocyanidin reductase from trifoliat...

文献信息： Wang, H. X., Ren, J., Zhou, S. Y., Duan, Y. Y., Zhu, C. Q., Chen, C. W., Liu, Z. Y., Zheng, Q. Y., Xiang, S., Xie, Z. Z., Wang, X., Chai, L. J., Ye, 等.  请登录

简介：Secretory structures in terrestrial plants serve as reservoirs for a variety of secondary metabolites. Among these, the secretory cavity of the Rutaceae family is notable for containing essential oils with a wide range of applications. However, the molecular basis underlying secretory cavity development is unknown. Here, we reveal a molecular framework for Citrus oil gland formation. Using genetic...

文献信息：Tian, Y. Z., Wang, X. Z., Huang, H. Q., Deng, X., Zhang, B. H., Meng, Y. X., Wu, L. B., Chen, H., Zhong, Y. and Chen, W. L.等.  请登录

简介：Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide. The etiological agent responsible for this disease is "Candidatus Liberibacter asiaticus" (CLas), a phloem-restricted bacterium transmitted by psyllid vectors. To date, effective practical strategies for curing Citrus HLB remain elusive. Additionally, no susceptibility genes associated with HLB have been identified in Cit...

文献信息：Tian, S. L., Yang, Y. Y., Fang, B., Uddin, S. and Liu, X. G.等.  请登录

简介：Citrus, cultivated extensively across the globe, possesses considerable economic importance and nutritional value. With the degradation of chlorophyll and accumulation of carotenoids, mature citrus fruits develop an orange-yellow peel, enhancing fruit value and consumer preference. MYB transcription factors (TFs) exert a significant role in diverse plant developmental processes and investigating t...

文献信息：Sun, Q., He, Z. C., Feng, D., Wei, R. R., Zhang, Y. Z., Ye, J. L., Chai, L. J., Xu, J., Cheng, Y. J., Xu, Q. and Deng, X. X.等.  请登录

简介：Carotenoid biosynthesis is closely associated with abscisic acid (ABA) during the ripening process of nonclimacteric fruits, but the regulatory mechanism that links ABA signaling to carotenoid metabolism remains largely unclear. Here, we identified two master regulators of ABA-mediated citrus fruit coloration, CsERF110 and CsERF53, which activate the expression of carotenoid metabolism genes (CsGG...