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  • 101. Citrus sinensis CBF1 Functions in Cold Tolerance by Modulating Putrescine Biosynthesis through Regulation of Arginine Decarboxylase

    文献信息:Song, Jie, Wu, Hao, He, Feng, Qu, Jing, Wang, Yue, Li, Chunlong and Liu, Ji-Hong等.  请登录

    简介:C-repeat (CRT) binding factors (CBFs) are well known to act as crucial transcription factors that function in cold stress response. Arginine decarboxylase (ADC)- mediated putrescine (Put) biosynthesis has been reported to be activated in plants exposed to cold conditions, but it remains elusive whether CBFs can regulate ADC expression and Put accumulation. In this study, we show that cold upregula...

    102. Biallelic Editing of the LOB1 YPromoter via CRISPR/Cas9 Creates Canker-Resistant 'Duncan' Grapefruit

    文献信息:Jia, Hongge, Omar, Ahmad A., Orbovic, Vladimir and Wang, Nian等.  请登录

    简介: Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus diseases worldwide. Generating disease-resistant citrus varieties is considered one of the most efficient and environmentally friendly measures for controlling canker. X. citri subsp. citri causes canker symptoms by inducing the expression of canker susceptibility gene LOB1 via PthA4, a transcription acti...

    103. Two AT-Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata

    文献信息:Dahro, Bachar, Wang, Yue, Khan, Madiha, Zhang, Yang, Fang, Tian, Ming, Ruhong, Li, Chunlong and Liu, Ji-Hong等.  请登录

    简介: Invertase (INV)-mediated sucrose (Suc) hydrolysis, leading to the irreversible production of glucose (Glc) and fructose (Frc), plays an essential role in abiotic stress tolerance of plants. However, the regulatory network associated with the Suc catabolism in response to cold environment remains largely elusive. Herein, the cold-induced alkaline/neutral INV gene PtrA/NINV7 of trifoliate orange (P...

    104. Reversion of fruit-dependent inhibition of flowering in Citrus requires sprouting of buds with epigenetically silenced CcMADS19

    文献信息: Mesejo, Carlos, Marzal, Andres, Martinez-Fuentes, Amparo, Reig, Carmina, de Lucas, Miguel, Iglesias, Domingo J., Primo-Millo, Eduardo, Blazquez, Migu等.  请登录

    简介:In Citrus, the response to environmental floral inductive signals is inhibited by the presence of developing fruits. The mechanism involves epigenetic activation of the CcMADS19 locus (FLC orthologue), encoding a floral repressor. To understand how this epigenetic regulation is reverted to allow flowering in the following season, we have forced precocious sprouting of axillary buds in fruit-bearin...

    105. Metabolic Profiling and Transcriptional Analysis of Carotenoid Accumulation in a Red-Fleshed Mutant of Pummelo (Citrus grandis)

    文献信息:Zhu, Congyi, Peng, Cheng, Qiu, Diyang and Zeng, Jiwu等.  请登录

    简介:Citrus grandis 'Tomentosa', commonly known as 'Huajuhong' pummelo (HJH), is used in traditional Chinese medicine and can moisten the lungs, resolve phlegm, and relieve coughs. A spontaneous bud mutant, named R-HJH, had a visually attractive phenotype with red albedo tissue and red juice sacs. In this study, the content and composition of carotenoids were investigated and compared between R-HJH and...

    106. Recovering triploid citrus hybrids from 2x x 2x sexual crosses with the aid of embryo rescue and flow cytometry in Turkey

    文献信息:Cimen, Berken, Yesiloglu, Turgut, Donmez, Dicle, Kacar, Yildiz Aka and Ercisli, Sezai等.  请登录

    简介:Background Turkey is one of the major exporters of mandarins in the Mediterranean region. Seedlessness in citrus, which is one of the most desired fruit quality traits, especially in fresh mandarin export markets, can be obtained via triploidy as in many fruit species. Triploid plants can be recovered by 2x x 2x hybridizations in citrus, as well as 2x x 4x and 4x x 2x crosses. Hence, we aimed to d...

    107. Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid

    文献信息: Hajeri, Subhas, Ng, James, Grosser, Jude and Vidalakis, Georgios等.  请登录

    简介:Viroids are RNA-based infectious agents that are single-stranded, covalently closed circular, non-coding, and naked. Unlike RNA viruses, which at least encode proteins for replication, encapsidation, and movement, lack of protein-coding capacity of viroids makes them completely reliant on host for replication and movement. The high genetic diversity in viroids is believed to be due to the absence ...

    108. Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis

    文献信息:Soriano, Leonardo, Omar, Ahmad A. and Martinelli, Adriana Pinheiro等.  请登录

    简介:Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and direct genetic transformation. These delicate and responsive materials are subjected to cell proliferation induction and differentiation of somatic embryos which further regenerate into entire plants. The isolation of viable protoplasts followed by re...

    109. Somatic Embryogenesis in Citrus (Citrus spp.), var. Valencia

    文献信息:de Melo Souza, Joanne Moraes, de Oliveira, Carolina Rossi, da Rocha Tavano, Eveline Carla, Soriano, Leonardo and Martinelli, Adriana Pinheiro等.  请登录

    简介:Somatic embryogenesis has been obtained in many citrus cultivars; however, the efficiency of the system is genotype dependent and culture synchronization is important to reach more efficient systems. In this chapter we present a detailed protocol of somatic embryogenesis induction from nucellar tissue and the use of an alternative method of callus sieving for culture synchronization and embryo pro...

    110. CsMYB96 confers resistance to water loss in citrus fruit by simultaneous regulation of water transport and wax biosynthesis

    文献信息:Zhang, Mingfei, Wang, Jinqiu, Liu, Ruilian, Liu, Hai, Yang, Hongbin, Zhu, Zhifeng, Xu, Rangwei, Wang, Pengwei, Deng, Xiuxin, Xue, Shaowu, Zhu, Feng an等.  请登录

    简介:CsMYB96 plays a role in regulating both APQ-mediated water movement and synthesis of cuticular wax to increase tolerance of citrus fruit to water loss. A Citrus sinensis R2R3 MYB transcription factor (CsMYB96) has previously been shown to be strongly associated with the expression of many genes related to wax biosynthesis in the fruit. In this study, CsMYB96 was found to alleviate water loss by si...

    111. Fast and eco-friendly method using atmospheric solids analysis probe mass spectrometry to characterize orange varieties

    文献信息: Pizzo, Jessica Santos, Silva, Jiuliane Martins, Silva Santos, Patricia Daniele, Visentainer, Jesui Vergilio and Santos, Oscar Oliveira等.  请登录

    简介:Orange fruit is one of the most popular types of fruit in the world, and its juice is the main product of its processing. This study aimed to evaluate a simple, fast, and eco-friendly methodology, atmospheric solids analysis probe mass spectrometry (ASAP-MS), to assess the chemical profile of four oranges varieties (Valencia, Folha Murcha, Pera, and Iapar). The oranges' varieties were evaluated fo...

    112. TRIPTYCHON-LIKE regulates aspects of both fruit flavor and color in citrus

    文献信息:He, Jiaxian, Xu, Yuantao, Huang, Ding, Fu, Jialing, Liu, Ziang, Wang, Lun, Zhang, Yin, Xu, Rangwei, Li, Li, Deng, Xiuxin and Xu, Qiang等.  请登录

    简介:The citrus transcription factor TRIPTYCHON-LIKE connects regulation of fruit flavor and color by competing with MYB activators; it forms a regulatory loop balancing citric acid and proanthocyanidin accumulation. Deciphering the genetic basis of organoleptic traits is critical for improving the quality of fruits, which greatly shapes their appeal to consumers. Here, we characterize the citrus R3-MY...

    113. miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus

    文献信息:Feng, Meng-Qi, Lu, Meng-Di, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Wang, Peng-Bo, Liu, Yue, Guo, Wen-Wu and Wu, Xiao-Meng等.  请登录

    简介:Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remai...

    114. Transgenic Sweet Orange Expressing the Sarcotoxin IA Gene Produces High-Quality Fruit and Shows Tolerance to 'Candidatus Liberibacter asiaticus'

    文献信息:Longhi, Talita Vigo, de Carvalho, Deived Uilian, Duin, Izabela Moura, da Cruz, Maria Aparecida and Leite Junior, Rui Pereira等.  请登录

    简介:Huanglongbing (otherwise known as HLB or greening) is currently the most devastating citrus disease worldwide. HLB is primarily associated with the phloem-inhabiting bacterium 'Candidatus Liberibacter asiaticus' (CLas). Currently, there are no citrus species resistant to CLas. Genetic transformation is one of the most effective approaches used to induce resistance against plant diseases. Antimicro...

    115. Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity

    文献信息: Liang, Beibei, Wan, Shiguo, Ma, Qingling, Yang, Li, Hu, Wei, Kuang, Liuqing, Xie, Jingheng, Liu, Dechun and Liu, Yong等.  请登录

    简介:Drought is one of the main abiotic stresses limiting the quality and yield of citrus. Cuticular waxes play an important role in regulating plant drought tolerance and water use efficiency (WUE). However, the contribution of cuticular waxes to drought tolerance, WUE and the underlying molecular mechanism is still largely unknown in citrus. 'Longhuihong' (MT) is a bud mutant of 'Newhall' navel orang...

    116. Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant and Its Wild Type

    文献信息:Mi, Lanfang, Ma, Dong, Lv, Shuping, Xu, Saibing, Zhong, Balian, Peng, Ting, Liu, Dechun and Liu, Yong等.  请登录

    简介: A complex molecular regulatory network plays an important role in the development and ripening of fruits and leads to significant differences in apparent characteristics. Comparative transcriptome and sRNAome analyses were performed to reveal the regulatory mechanisms of fruit ripening in a spontaneous early-ripening navel orange mutant ('Ganqi 4', <i>C</i><i>itrus sinensis</i> L. Osbeck) and its...

    117. A versatile Agrobacterium-based plant transformation system for genetic engineering of diverse citrus cultivars

    文献信息: Dominguez, M. M., Padilla, C. S. and Mandadi, K. K.等.  请登录

    简介:Developing an efficient transformation system is vital in genetically engineering recalcitrant crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based stable plant transformation methodology for citrus genetic engineering. The process was optimized to suit the requirements of fourteen citrus varieties by establishing appropriate infection, co-cultivation, selection, and cult...

    118. LbCas12a-D156R Efficiently Edits LOB1 Effector Binding Elements to Generate Canker-Resistant Citrus Plants

    文献信息: Jia, Hongge, Wang, Yuanchun, Su, Hang, Huang, Xiaoen and Wang, Nian等.  请登录

    简介:Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is an economically important disease in most citrus production regions worldwide. Xcc secretes a transcriptional activator like effector (TALE) PthA4 to bind to the effector binding elements (EBEs) in the promoter region of canker susceptibility gene LOB1 to activate its expression, which in turn causes canker symptoms. Editing the EBE r...

    119. 无核柑桔新品种——华柑4号

    文献信息: 吴巨勋 and 伊华林等.  请登录

    简介:华柑4号”是从靖安椪柑(Citrus reticulata Blanco.)芽变中选育出来的无核柑桔新品种。2021年8月通过非主要农作物品种登记,登记编号为GPD柑橘(2021)420013。该品种果实成熟时果皮色泽呈黄橙色,果皮较薄、较光滑,果皮厚度2.0 mm,易剥皮,油胞较小,果形指数0.80,果实扁圆形,单果重124.81 g,可溶性固形物含量13.2%,可滴定酸含量1.17%,维生素C含量为30.77 mg/100 g,平均单果种子数为0.19粒,混栽或单独栽培均接近无核。

    120. 四个柑橘杂交组合F1代果实基本性状遗传分析及无核优株筛选

    文献信息:张文龙等.  请登录

    简介:柑橘种间杂交甚至属间杂交没有生殖隔离,遗传背景丰富多彩,杂交育种能否成功的关键在于亲本的选择。因此,对杂交组合F1代群体鉴定评价并总结分析杂种后代的遗传规律是必不可少的工作,为今后定向选育出符合育种目标的优质单株奠定工作基础,也为现代分子生物学的大数据育种积累素材。在柑橘育种中,无核或少核是柑橘品种选育的重点和热点,是商业化的育种目标之一。根据多年的观察和测定,本试验选择以下四个杂交组合:‘爱媛28’ב春见’...

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