文献信息：Shi Cai-Yun, Hussain Syed Bilal, Yang Huan, Bai Ying-Xin, Khan Muhammad Abbas ,Liu Yong-Zhong等.  发表于【Plant Science,<Go to ISI>://WOS:000494891900026】 | 请登录

简介：Citric acid homeostasis patterns and its content are diversified among the fruits of citrus cultivars, but the cause remained unclear. In this study we showed that changes of citric acid content were highly associated with the expression profiles of a P-type proton pump gene (CsPH8) in the fruits of six citrus cultivars; moreover, analysis of 21 different fruit samples indicated that the correlati...

文献信息：Liu Xiao, Guo Ling-Xia, Luo Li-Juan, Liu Yong-Zhong, Peng Shu-Ang等.  发表于【Plant Molecular Biology】 | 请登录

简介：Key message: At least eight MGT genes were identified in citrus and PtrMGT5 plays important role in maintaining Mg homeostasis in citrus by getting involved in the Mg absorption and transport. Abstract: Magnesium (Mg) is an essential macronutrient for plant growth and development, and the magnesium transporter (MGT) genes participate in mediate Mg2+ uptake, translocation and sequestration into cel...

文献信息：Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2020-07-19 发表于【Tree physiology】 | 请登录

简介：Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants ...

文献信息：Hasan Nazmul, Kamruzzaman Mohammad, Islam Shariful, Hoque Hammadul, Bhuiyan Fahmid Hossain, Prodhan Shamsul H.等.  2020-10-16 发表于【Journal of Genetic Engineering and Biotechnology】 | 请登录

简介：Background Recent studies indicate that farmers are facing several challenges due to biotic and abiotic stresses like diseases, drought, cold, and soil salinity which are causing declined Citrus production. Thus, it is essential to improve these varieties which would be resistant against biotic and abiotic stresses as well as high yielding. The transformation of abiotic stress tolerant genes in Ci...

文献信息：Huang Ding, Yuan Yue, Tang Zhouzhou, Huang Yue, Kang Chunying, Deng Xiuxin, Xu Qiang等.  发表于【Plant Cell and Environment】 | 请登录

简介：Blood orange is generally recognized to accumulate anthocyanins in its fruit pulp in a cold-inducible manner. We observed that the fruit peel of blood orange can also accumulate anthocyanins under ample light conditions. Interestingly, purple pummelo can accumulate anthocyanins only in its fruit peel but not in its pulp. The mechanism underlying the tissue specificity of anthocyanin accumulation i...

文献信息：Killiny Nabil, Nehela Yasser等.  发表于【Plant Cell Reports】 | 请登录

简介：Key message: In citrus, abscisic acid-deficiency was associated with a dwarfing phenotype, slow growth, small leaves, decreased fresh weight, and faster water loss. ABA supplementation reversed the dwarfing phenotype and enhanced growth.AbstractAbscisic acid (ABA) is a ubiquitously distributed phytohormone, which is almost produced by all living kingdoms. In plants, ABA plays pleiotropic physiolog...

文献信息：Xie Kai-Dong ,Yuan Dong-Ya, Wang Wei, Xia Qiang-Ming, Wu Xiao-Meng ,Chen Chuan-Wu, Chen Chun-Li ,Grosser Jude W, Guo Wen-Wu等.  2019-06-27 发表于【Scientia Horticulturae】 | 请登录

简介：Seedlessness is a primary breeding objective for citrus fresh fruit market, and triploids have been proven to have great value to produce seedless fruits. In this study, aiming to produce triploid plants for developing some seedless cultivars, four 2x x 4x interploid crosses were conducted using three elite but seedy cultivars as seed parents and one newly flowered doubled diploid and two allotetr...

文献信息：Xiongjie Zheng,Kaijie Zhu,Quan Sun,Weiyi Zhang,Xia Wang,Hongbo Cao,Meilian Tan,Zongzhou Xie,Yunliu Zeng,Junli Ye,Lijun Chai,Qiang Xu,Zhiyong Pan,Shuny等.  请登录

简介：Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference. Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments. In this study, using an integrated genetic approach we revealed that a 5' cis-regulatory change at CCD4b encoding CAROTE...

文献信息：邹修平,龙俊宏,彭爱红,陈敏,龙琴,陈善春等.  2019-11-01 发表于【中国农业科学】 | 请登录

简介：背景: 由柑橘黄单胞杆菌柑橘亚种（Xanthomonas citri subsp. citri）引起的柑橘溃疡病是柑橘生产上最具毁灭性的一种病害。植物生长素在调控柑橘溃疡病菌引起的寄主侵染部位脓疱形成中起重要作用。生长素早期响应基因GH3通过酰基化吲哚-3-乙酸（indole-3-acetic acid, IAA）调控植物激素动态平衡。前期研究发现柑橘CsGH3.6在调控生长素响应溃疡病侵染中起着重要作用。目的: 通过对超量表达CsGH3.6转基因晚锦橙的抗病性、植株表型、细...

文献信息：王丽娟,龙俊宏,谢竹,吴柳,彭爱红,何永睿,龙琴,陈善春,邹修平等.  发表于【园艺学报】 | 请登录

简介：由柑橘黄单胞杆菌致病变种(Xanthomonas citri subsp. citri,Xcc)引起的柑橘溃疡病严重危害着柑橘产业的健康可持续发展。前期研究表明,CsWRKY22可能是溃疡病感病基因。从‘晚锦橙’(Citrus sinensis Osbeck)中克隆了CsWRKY22的两个等位启动子pCsWRKY22-1和pCsWRKY22-2,长度分别为1 428和1406bp。序列分析表明两个等位启动子序列一致性为91.76%。两个等位启动子含有多种参与植物防御反应和生长发育的顺式作用元件,相同元件的数量和位...

文献信息：胡安华,祁静静,张庆雯,陈善春,邹修平,许兰珍,彭爱红,雷天刚,姚利晓,龙琴,何永睿,李强等.  发表于【中国农业科学】 | 请登录

简介：【目的】克隆CsPGIP并分析其表达特性,转化柑橘得到超表达转基因株系,并进行柑橘溃疡病抗性评价,为柑橘溃疡病分子育种提供理论依据。【方法】从晚锦橙和四季橘中克隆柑橘CsPGIP,使用MEGA6进行多序列比对并构建系统发育树;采用在线软件BaCelLo和SignalP 4.0进行亚细胞定位和信号肽预测并用GFP瞬时表达确定CsPGIP在细胞内的定位;利用实时荧光定量PCR(qRT-PCR)比较接种溃疡病菌前后高感品种和高抗品种中柑橘CsPGIP的表达特性,分析溃疡病...

文献信息：葛廷,黄雪,谢让金等.  发表于【中国农业科学】 | 请登录

简介：【目的】多聚半乳糖醛酸酶是一类参与细胞壁降解的水解酶,在植物生长发育和器官脱落过程中发挥着重要作用。本研究克隆柑橘CitPG34及其启动子(CitPG34-P)并进行表达分析,为深入研究柑橘PG在幼果脱落过程的生物功能奠定基础。【方法】以‘塔罗科’血橙(Citrus sinensis L. Osbeck)为材料,克隆CitPG34及其启动子,利用ProtParam、Cello、CLUSTALX、MEGA5.2、PlantCARE等软件对其蛋白特性及启动子顺式作用元件进行分析预测;利用实时荧光定...

文献信息：Soler Nuria, Plomer Montserrat, Fagoaga Carmen, Moreno Pedro, Navarro Luis, Flore, Ricardo, Pena Leandro等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介：Conventional breeding of citrus types demands a long-term effort due to their complex reproductive biology and long juvenile period. As a compelling alternative, genetic engineering of mature tissues allows the insertion of specific traits into specific elite cultivars, including well-known and widely grown varieties and rootstocks, thus reducing the time and costs involved in improving and evalua...

文献信息：Singh Gurteg, Aulakh Pushpinder Singh, Sarao Navraj Kaur, Rattanpal Harinder Singh, Sidhu Gurupkar Singh等.  发表于【Agricultural Research】 | 请登录

简介：The molecular verification of different intra-specific mandarin hybrids was explored during 2014-2015. Simple sequence repeat (SSR) markers were employed to identify putative zygotic seedlings from hybrid progeny produced from different crosses in mandarin. The most informative SSR marker in the F-1 hybrids of "Daisy x Kinnow' cross was CCSM170 which identified 73% of zygotic seedlings followed by...

文献信息：Shimo Hugo Massayoshi, Terassi Carolina, Lima Silva Caio Cesar, de Lima Zanella Jackeline, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedet等.  2019-03-21 发表于【Molecular plant pathology】 | 请登录

简介： Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unk...

文献信息：Li Shao-Jia, Xie Xiu-Lan, Liu Sheng-Chao, Chen Kun-Song, Yin Xue-Ren等.  2019-11-30 发表于【 Food chemistry】 | 请登录

简介：Citrus fruit postharvest degreening is a critical stage in marketing, carried out by exposure to ethylene or ethephon. Genome-wide screening of the AP2/ERF superfamily indicated that a novel ERF-II (CitERF6) was shown to trans-activate the CitPPH promoter. Expression of CitERF6 is associated with both developmental and postharvest degreening in citrus fruit. Transient and stable over-expression of...

文献信息：Killiny Nabil, Nehela Yasser, Hijaz Faraj, Ben-Mahmoud Sulley K., Hajeri Subhas, Gowda Siddarame等.  发表于【Plant Biotechnology Reports】 | 请登录

简介：In this study, phytoene desaturase (PDS) in citrus and green fluorescent protein (GFP) in Nicotiana benthamiana (line 16c) were silenced by virus-induced gene silencing (VIGS) with Citrus tristeza virus (CTV), using the sense and antisense orientations. The antisense orientation showed a stronger reduction of GFP expression in N. benthamiana compared with the sense orientation. Similarly, the anti...

文献信息： Kanwar Jyoti, Kaul M. K., Naruka I. S., Singh P. P.等.  发表于【Indian Journal of Agricultural Sciences】 | 请登录

简介：This study was caned out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0....

文献信息：Jia Ning, Liu Jiqin, Tan Penghui, Sun Yufeng, Lv Yuemeng, Liu Jiameng, Sun Jing, Huang Yatao, Lu Jia, Jin Nuo, Li Minmin, Imam Khandaker M, Xin Fengji等.  2019-02-25 发表于【Frontiers in Plant Science】 | 请登录

简介：Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a c...

文献信息：Jia Hongge, Zou Xiuping, Orbovic Vladimir, Wang Nian等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介： CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.
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