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  • 241. Analysis of crossover events and allele segregation distortion in interspecific citrus hybrids by single pollen genotyping

    文献信息:Garavello, Miguel Cuenca, Jose Dreissig, Steven Fuchs, Joerg Navarro, Luis Houben, Andreas Aleza, Pablo等.  2020-05-25 发表于【Frontiers in Plant Science】 | 请登录

    简介:In citrus, a classical method of studying crossovers and segregation distortion (SD) is the genetic analysis of progenies. A new strategy combining fluorescence-activated cell sorting and whole genome amplification of haploid pollen nuclei with a large set of molecular markers, offers the opportunity to efficiently determine the frequency of crossovers and the identification of SD without the need...

    242. Phylogenetic relationships among accessions in Citrus and related genera based on the insertion polymorphism of the CIRE1 retrotransposon

    文献信息:Horibata Akira, Kato Tsuneo等.  发表于【Open Agriculture】 | 请登录

    简介:A total of 145 accessions of the genus Citrus and related genera, maintained in the Conservation Garden for Citrus Germplasm at the Experimental Farm of Kindai University, Yuasa, Wakayama, Japan, were examined for their phylogenetic relationships. The present classification was conducted using an inter-retrotransposon amplified polymorphism (IRAP) method based on the insertion polymorphism of a re...

    243. Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis): an agro-economically important variety

    文献信息:Jardak Rahma, Boubakri Hatem, Zemni Hassene, Gandoura Samia, Mejri Samiha, Mliki Ahmed, Ghorbel Abdelwahed等.  2020-02-06 发表于【3 Biotech】 | 请登录

    简介:An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incubating epicotyl explants (excised in an oblique manner) in MT culture media contai...

    244. Generation of homozygous canker-resistant citrus in the T0 generation using CRISPR-SpCas9p

    文献信息:Jia Hongge, Wang Nian等.  发表于【Plant Biotechnology Journal】 |  , 请登录

    245. Evaluation of seedlings from 11 citrus accessions for in vivo micrografting

    文献信息:Le Minh-Ly, Sakai Kaori, Mizunoe Yuki, Ozaki Yukio, Wakana Akira等.  发表于【Horticulture Journal】 | 请登录

    简介:The very young seedlings of trifoliate orange, and its hybrids with trifoliate leaves as a marker, are usually used as a rootstock for in vitro and in vivo micrografting of citrus to eliminate viruses in spring in temperate regions. In tropical and subtropical regions or in summer, however, the production and use of trifoliate orange seedlings is difficult. Therefore, it is necessary to establish ...

    246. ClPLD5, a phospholipase gene is involved in protection of cytoskeleton stability in pollen of self-imcompatible 'Xiangshui' lemon (Citrus limon)

    文献信息:Lin Wei, Minhphu Do, Wei Mi, He Xinhua, Liu Yuan, Fan Zhiyi, Zhang Shuwei, Luo Cong, Huang Guixiang, Hu Guibing等.  发表于【Plant Cell Tissue and Organ Culturerrk7】 | 请登录

    简介:AC2-PLD gene (ClPLD5) related to self-incompatibility was identified within the "Xiangshui" lemon C2-PLD family, and overexpression of the ClPLD5 gene in pollen increased the stability of the pollen cytoskeleton under self-incompatibility challenge. Self-incompatibility (SI) is an important mechanism for the prevention of self-fertilization in plants and is one of the major causes of seedlessness ...

    247. Up-regulation of microRNA targets correlates with symptom severity in Citrus sinensis plants infected with two different isolates of citrus psorosis virus

    文献信息:Marmisolle Facundo E., Arizmendi Ailin,Ribone Andres, Rivarola Maximo, Garcia Maria L., Reyes Carina A.等.  发表于【Planta】 | 请登录

    简介:Sweet orange (Citrus sinensis) may suffer from disease symptoms induced by virus infections, thus resulting in drastic economic losses. Infection of sweet orange plants with two isolates of citrus psorosis virus (CPsV), expressing different symptomatologies, alters the accumulation of a set of endogenous microRNAs (miRNAs). Here, we predicted ten putative targets from four down-regulated miRNAs: t...

    248. Substantial equivalence of a transgenic Lemon fruit showing postharvest fungal pathogens resistance

    文献信息:Muccilli Vera, Vitale Alessandro, Sheng Ling, Gentile Alessandra, Cardullo Nunzio, Tringali Corrado, Oliveri Cinzia, La Rosa Rosa, Di Guardo Mario, La等.  2020-03-25 发表于【Journal of Agricultural and Food Chemistry】 | 请登录

    简介:The development of genetically modified (GM) crops speeds up the obtainment of novel varieties with improved agronomic characteristics. However, the risk evaluation of the use of GMs is mandatory before their release in the market. In this paper, an untargeted and comprehensive nuclear magnetic resonance-based metabolomic study was carried out on the peel and flesh of a transgenic lemon clone (E23...

    249. Citrus CsACD2 is a target of Candidatus Liberibacter asiaticus in Huanglongbing disease

    文献信息:Pang Zhiqian ,Zhang Li, Coaker Gitta Laurel, Ma Wenbo. He Sheng Yang ,Wang Nian等.  发表于【Plant Physiology】 | 请登录

    简介:Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (Las), is one of the most destructive citrus diseases worldwide, yet how Las causes HLB is poorly understood. Here we show that a Las-secreted protein, SDE15 (CLIBASIA_04025), suppresses plant immunity and promotes Las multiplication. Transgenic expression of SDE15 in Duncan grapefruit (Citrus × paradisi) suppresses the hyper...

    250. Citrus reticulata CrRAP2.2 transcriptional factor shares similar functions to the Arabidopsis homolog and increases resistance to Xylella fastidiosa

    文献信息:Pereira Willian,Takita Marco, Melotto Maeli, de Souza Alessandra等.  发表于【Molecular Plant-Microbe Interactions,<Go to ISI>://WOS:000551075100014】 | 请登录

    简介:Xylella fastidiosa is a worldwide multihost pathogen that causes diseases in different crops. It is considered a new global threat and substantial efforts have been made in order to identify sources of resistance. Indeed, many genes have been associated with resistance to X. fastidiosa, but without functional validation. Here, we describe a C. reticulata gene homologous to the transcriptional fact...

    251. Potential mechanisms of AtNPR1 mediated resistance against Huanglongbing (HLB) in Citrus

    文献信息:Qiu Wenming ,Soares Juliana ,Pang Zhiqian, Huang Yixiao, Sun Zhonghai ,Wang Nian, Grosser Jude, Dutt Manjul等.  发表于【International Journal of Molecular Sciences】 | 请登录

    简介:Huanglongbing (HLB), a bacterial disease caused by Candidatus Liberibacter asiaticus (CLas), is a major threat to the citrus industry. In a previous study conducted by our laboratory, several citrus transgenic trees expressing the Arabidopsis thaliana NPR1 (AtNPR1) gene remained HLB-free when grown in a field site under high HLB disease pressure. To determine the molecular mechanisms behind AtNPR1...

    252. Increased drought and salinity tolerance in Citrus aurantifolia (Mexican Lemon) plants overexpressing Arabidopsis CBF3 gene

    文献信息:Jesús L. Romero-Romero, Claudio Inostroza-Blancheteau, Marjorie Reyes-Díaz ,Juan Pablo Matte ,Felipe Aquea, Carmen Espinoza, Pilar M. Gil, Patricio Ar等.  发表于【Journal of Soil Science and Plant Nutrition】 | 请登录

    简介:Citrus are a globally important fruit crop. Abiotic stressors such as drought and salinity adversely affect physiological citrus performance and survival. With the aim of improving drought tolerance in citrus plants, we constructed transgenic lines of Citrus lemon overexpressing the Arabidopsis transcription factor CBF3. Molecular, physiological, and quantitative real-time analyses showed high exp...

    253. Recombinant expression, characterization and phylogenetic studies of novels cystatins-like proteins of sweet orange (Citrus sinensis) and clementine (Citrus clementina)

    文献信息:Schneider Vanessa Karine, da Silva Ferrara Taise Fernanda, Rocha Samara Vieira, Santos-Junior Celio Dias, Neo-Justino Daniela Morilha, da Cunha Anders等.  2020-06-01 发表于【International Journal of Biological Macromolecules,<Go to ISI>://WOS:000530068000056】 | 请登录

    简介:Phytocystatins are plant cystatins that are related to several physiological processes regulating endogenous cysteine proteases involved in seed development and germination, programmed cell death and response to stress conditions. In addition, phytocystatins can act in plant defense against exogenous peptidases from herbivorous insects, pathogens and nematodes. Considering that Citrus fruits are i...

    254. Development of genetically modified citrus plants for the control of citrus canker and huanglongbing

    文献信息:Soares Juliana M., Tanwir Sameena E., Grosser Jude W., Dutt Manjul等.  发表于【Tropical Plant Pathology】 | 请登录

    简介:Citrus cultivation is challenging due to the plethora of abiotic and biotic stresses faced by the crop. In recent years, production has been severely affected by diseases such as citrus canker and huanglongbing (HLB). Disease management is hampered as there is no field resistance to these diseases in any of the important commercially planted varieties. Traditionally, conventional breeding approach...

    255. The miR399-CsUBC24 module regulates reproductive development and male fertility in citrus

    文献信息:Wang Rong ,Fang Yan-Ni ,Wu Xiao-Meng, Qing Mei, Li Chao-Chao, Xie Kai-Dong ,Deng Xiuxin, Guo Wen-Wu等.  发表于【Plant Physiology】 | 请登录

    简介:MicroRNA399 (miR399) regulates phosphate homeostasis in plants by down-regulating the expression of PHOSPHATE2 (PHO2, or UBC24 encoding the ubiquitin-conjugating E2 enzyme). We previously identified CsmiR399a.1 in a small RNA sequencing screen of a male-sterile somatic cytoplasmic hybrid (or cybrid) of pummelo (Citrus grandis). Here, we report that miR399 affects reproductive development and male ...

    256. Characterization of a caffeoyl-CoA O-methyltransferase-like enzyme involved in biosynthesis of polymethoxylated flavones in Citrus reticulata

    文献信息:Liu Xiaojuan, Zhao Chenning ,Gong Qin ,Wang Yue, Cao Jinping ,Li Xian, Grierson Donald ,Sun Chongde等.  发表于【Journal of experimental botany】 | 请登录

    简介:Polymethoxylated flavones (PMFs), which accumulate exclusively in fruit peel of citrus, play important physiological and pharmacological roles but the genetic basis for the methylation of flavonoids has not been fully elucidated in citrus. Here we characterize a caffeoyl-CoA O-methyltransferase-like enzyme, designated CrOMT1. The expression pattern of CrOMT1 was highly correlated with the concentr...

    257. Fruit characteristics and seed anatomy of 'Majia' pomelo pollinated with cobalt-60 gamma-ray-irradiated pollen

    文献信息:Yang Li, Liu Dechun, Hu Wei, Chun Yongqiang, Zhang Juanjuan, Liu Yong等.  2020-06-01 发表于【Scientia Horticulturae】 | 请登录

    简介:The 'Majia' pomelo (Citrus maxima (Burm.) Merr., Rutaceae) is a distinctive local cultivar of Jiangxi province, China, that is famous for its good flavour and abundant lycopene content; however, each 'Majia' pomelo fruit usually contains approximately 120-200 large seeds. To obtain seedless 'Majia' pomelo fruit, we used pollen from sour pomelo (a local variety generally used for pollination) irrad...

    258. Chimerism evaluation of 'Hongrou Huyou', a grafted chimera between Citrus changshan-huyou and Citrus unshiu

    文献信息:Zhang Min,Zhang Zequn,Wu Qun, Ke Fuzhi ,Xu Jianguo, Zhao Siqing, Wang Gang ,Zhang Chi等.  发表于【Horticultural Science】 | 请登录

    简介:Chimeras occur spontaneously or artificially and are valuable in horticultural crop breeding. A new diploid citrus chimera, named `Hongrou Huyou' (abbreviated HH) (Citrus changshan-huyou + C. unshiu), was found during a bud sports investigation in China. The morphology, flesh carotenoid content, and molecular markers were evaluated in HH and the two grafted donors. The results showed that characte...

    259. The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus

    文献信息:Zhang Pan, Liu Xiaofeng, Yu Xin, Wang Fusheng, Long Junhong,Shen Wanxia,Jiang Dong,Zhao Xiaochun等.  2020-07-06 发表于【BMC Plant Biology】 | 请登录

    简介:Background: Limonoids are major bioactive compounds that are produced by the triterpenoid metabolic pathway. The detailed biochemical process of limonoid biosynthesis and the mechanism of its molecular regulation remain elusive. The identification of transcription factors that regulate limonoid biosynthetic pathways is very important for understanding the underlying regulatory mechanisms. This inf...

    260. The citrus CsPH4-Noemi regulatory complex is involved in proanthocyanidin biosynthesis via a positive feedback loop

    文献信息:Yin Zhang,Junli Ye,Chaoyang Liu,Qiang Xu,Lichang Long,Xiuxin Deng等.  请登录

    简介:Proanthocyanidins (PAs; or condensed tannins) are a major class of flavonoids that contribute to citrus fruit quality. However, the molecular mechanism responsible for PA biosynthesis and accumulation in citrus remains unclear. Here, we identify a PH4–Noemi regulatory complex that regulates proanthocyanidin biosynthesis in citrus. Overexpression of PH4 or Noemi in citrus calli activated the expre...

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