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    1. Reaction of transgenic Citrus sinensis plants to Citrus tristeza virus infection by Toxoptera citricida

    文献信息:Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

    简介:Transgenic Citrus sinensis \'Hamlin\' and \'Valencia\' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detectio...

    2. Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions

    文献信息:Martins, C. P. Neves, D. M. Cidade, L. C. Mendes, A. F. Silva, D. C. Almeida, A. F. Coelho-Filho, M. A. Gesteira, A. S. Soares-Filho, W. S. Costa, M. 等.  2017-01-21 发表于【Planta】 |  , 请登录

    简介:Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing di...

    3. 柑橘果实特异表达基因CsPMEI1在拟南芥和金柑中的过量表达研究

    文献信息:张红红等.   , 请登录

    4. Methods for Producing Transgenic Plants Resistant to CTV

    文献信息:Soler Nuria, Plomer Montserrat, Fagoaga Carmen, Moreno Pedro, Navarro Luis, Flore, Ricardo, Pena Leandro等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

    简介:Conventional breeding of citrus types demands a long-term effort due to their complex reproductive biology and long juvenile period. As a compelling alternative, genetic engineering of mature tissues allows the insertion of specific traits into specific elite cultivars, including well-known and widely grown varieties and rootstocks, thus reducing the time and costs involved in improving and evalua...

    5. Molecular Verification of Putative Zygotic Seedlings in Different Intra-Specific Crosses in Mandarins (Citrus reticulata) by SSR Markers

    文献信息:Singh Gurteg, Aulakh Pushpinder Singh, Sarao Navraj Kaur, Rattanpal Harinder Singh, Sidhu Gurupkar Singh等.  发表于【Agricultural Research】 | 请登录

    简介:The molecular verification of different intra-specific mandarin hybrids was explored during 2014-2015. Simple sequence repeat (SSR) markers were employed to identify putative zygotic seedlings from hybrid progeny produced from different crosses in mandarin. The most informative SSR marker in the F-1 hybrids of "Daisy x Kinnow' cross was CCSM170 which identified 73% of zygotic seedlings followed by...

    6. Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance

    文献信息:Shimo Hugo Massayoshi, Terassi Carolina, Lima Silva Caio Cesar, de Lima Zanella Jackeline, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedet等.  2019-03-21 发表于【Molecular plant pathology】 | 请登录

    简介: Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unk...

    7. Auto- and mutual-regulation between two CitERFs contribute to ethylene-induced citrus fruit degreening

    文献信息:Li Shao-Jia, Xie Xiu-Lan, Liu Sheng-Chao, Chen Kun-Song, Yin Xue-Ren等.  2019-11-30 发表于【 Food chemistry】 | 请登录

    简介:Citrus fruit postharvest degreening is a critical stage in marketing, carried out by exposure to ethylene or ethephon. Genome-wide screening of the AP2/ERF superfamily indicated that a novel ERF-II (CitERF6) was shown to trans-activate the CitPPH promoter. Expression of CitERF6 is associated with both developmental and postharvest degreening in citrus fruit. Transient and stable over-expression of...

    8. Citrus tristeza virus-based induced gene silencing of phytoene desaturase is more efficient when antisense orientation is used

    文献信息:Killiny Nabil, Nehela Yasser, Hijaz Faraj, Ben-Mahmoud Sulley K., Hajeri Subhas, Gowda Siddarame等.  发表于【Plant Biotechnology Reports】 | 请登录

    简介:In this study, phytoene desaturase (PDS) in citrus and green fluorescent protein (GFP) in Nicotiana benthamiana (line 16c) were silenced by virus-induced gene silencing (VIGS) with Citrus tristeza virus (CTV), using the sense and antisense orientations. The antisense orientation showed a stronger reduction of GFP expression in N. benthamiana compared with the sense orientation. Similarly, the anti...

    9. In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants

    文献信息: Kanwar Jyoti, Kaul M. K., Naruka I. S., Singh P. P.等.  发表于【Indian Journal of Agricultural Sciences】 | 请登录

    简介:This study was caned out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0....

    10. Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors

    文献信息:Jia Ning, Liu Jiqin, Tan Penghui, Sun Yufeng, Lv Yuemeng, Liu Jiameng, Sun Jing, Huang Yatao, Lu Jia, Jin Nuo, Li Minmin, Imam Khandaker M, Xin Fengji等.  2019-02-25 发表于【Frontiers in Plant Science】 | 请登录

    简介:Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a c...

    11. Genome Editing in Citrus Tree with CRISPR/Cas9

    文献信息:Jia Hongge, Zou Xiuping, Orbovic Vladimir, Wang Nian等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

    简介: CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.
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    12. CRISPR-LbCas12a-mediated modification of citrus

    文献信息:Jia Hongge, Orbovi Vladimir, Wang Nian等.  2019-03-25 发表于【Plant biotechnology journal】 | 请登录

    简介: Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully in Duncan grapefruit via Xcc-facilitated agroinfiltration. Next, LbCas12a drive...

    13. 'Candidatus Liberibacter asiaticus' peroxiredoxin (LasBCP) suppresses oxylipin-mediated defense signaling in citrus

    文献信息:Jain Mukesh, Munoz-Bodnar Alejandra, Gabriel Dean W.等.  发表于【Journal of Plant Physiology】 | 请登录

    简介:The Lasbcp (CLIBASIA_RS00445) 1-Cys peroxiredoxin gene is conserved among all 13 sequenced strains of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing or "citrus greening" disease. LasBCP was previously characterized as a secreted peroxiredoxin with substrate specificity for organic peroxides, and as a potential pathogenicity effector. Agrobacterium-mediated transient expressio...

    14. Retrotransposon promoter of Ruby1 controls both light- and cold-induced accumulation of anthocyanins in blood orange

    文献信息:Huang Ding, Yuan Yue, Tang Zhouzhou, Huang Yue, Kang Chunying, Deng Xiuxin, Xu Qiang等.  2019-07-15 发表于【 Plant, cell & environment】 | 请登录

    简介:Blood orange is generally recognized to accumulate anthocyanins in its fruit pulp in a cold-inducible manner. We observed that the fruit peel of blood orange can also accumulate anthocyanins under ample light conditions. Interestingly, purple pummelo can accumulate anthocyanins only in its fruit peel but not in its pulp. The mechanism underlying the tissue specificity of anthocyanin accumulation i...

    15. Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance

    文献信息: He Yongrui, Jia Ruirui, Qi Jingjing, Chen Shanchun, Lei Tiangang, Xu Lanzhen, Peng Aihong, Yao Lixiao, Long Qin, Li Zhengguo, Li Qiang等.  2019-07-30 发表于【Gene】 | 请登录

    简介:Genetic engineering approaches offer an alternative method to the citrus canker resistance breeding. The ethylene response factor (ERF) family is a member of families of transcription factors that are particular to plants and contribute significantly to biotic stress response and to plant growth. CsAP2-09 belongs to the citrus AP2/ERF transcription factor family. Initially, we proved the induction...

    16. CRISPR/Cas9介导柑橘CsLOB1基因启动子的多位点编辑

    文献信息:邹修平, 范迪, 彭爱红, 何永睿, 许兰珍, 雷天刚, 姚利晓, 李强, 罗克明, 陈善春等.  请登录

    简介:为了获得CsLOB1启动子较大片段删除的突变体,利用CRISPR/Cas9技术对CsLOB1启动子进行多位点编辑。通过在CsLOB1启动子的EBEPthA4区域及其上、下游设计不同的靶标位点,构建了2个植物表达载体pCas9CsLOB1:2sites和pCas9CsLOB1:3sites,分别对CsLOB1启动子同时进行2个位点和3个位点的编辑。测序结果表明pCas9CsLOB1:2sites和pCas9CsLOB1:3sites的基因编辑效率分别为64.7%和80.0%,突变体植株在2个sgRNA之间发生了DNA片段的删除。进一步的分...

    17. Evaluation of polyembryony for genetic resources and efficacy of simple sequence repeat markers for the identification of nucellar and zygotic embryo&#8209;derived individuals in citrus

    文献信息:김호방等.  发表于【Applied Biological Chemistry】 | 请登录

    简介:Many citrus cultivars have the polyembryony trait that develops many nucellar embryos alongside a single zygotic embryo in an individual seed by sporophytic apomixis. This unique botanical trait hinders citrus breeding by genetic hybridization and affects breeding efficiency and cost. Techniques to efficiently identify nucellar and zygotic individuals in citrus are still very limited. For a system...

    18. Overexpression of PtrbHLH, a basic helix-loop-helix transcription factor from Poncirus trifoliata, confers enhanced cold tolerance in pummelo (Citrus grandis) by modulation of H2O2 level via regulatin

    文献信息:Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2019-07-19 发表于【Tree physiology】 | 请登录

    简介: Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants...

    19. RNA interference and CRISPR: Promising approaches to better understand and control citrus pathogens

    文献信息:Goulin Eduardo Henrique, Galdeano Diogo Manzano, Granato Lais Moreira, Matsumura Emilyn Emy, Dalio Ronaldo Jose Durigan, Machado Marcos Anton等.  2019-09-01 发表于【Microbiological research】 | 请登录

    简介: Citrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRIS...

    20. Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance

    文献信息: de Lima Silva Caio Cesar, Shimo Hugo Massayoshi, de Felicio Rafael, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedetti Celso Edua等.  2019-03-07 发表于【 Scientific Reports】 | 请登录

    简介:Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that...

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