文献信息：Gong, J. L., Chen, Y. S., Xu, Y. N., Gu, M. F., Ma, H. J., Hu, X. L., Li, X. L., Jiao, C. and Sun, X. P.等.  请登录

简介： Due to the protracted transgenic timeline and low efficiency in stable genetic transformation of woody plants, there has been limited exploration of real-time organelle imaging within stable transgenic woody plant cells. Here, we established an efficient in vivo genetic transformation system for woody plants using an Agrobacterium rhizogenes-mediated approach. This system was successfully validat...

文献信息：Zhang, Shushe, Wang, Xuefeng, Zhao, Tingchang and Zhou, Changyong等.  请登录

简介：Citrus huanglongbing (HLB) has been causing enormous damage to the global citrus industry. As the main causal agent, 'Candidatus Liberibacter asiaticus' (CLas) delivers a set of effectors to modulate host responses, while the modes of action adopted remain largely unclear. Here, we demonstrated that CLIBASIA_00185 (CLas0185) could attenuate reactive oxygen species (ROS)-mediated cell death in Nico...

文献信息：Su, Hang, Wang, Yuanchun, Xu, Jin, Omar, Ahmad A., Grosser, Jude W. and Wang, Nian等.  请登录

简介： Introduction: Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a devastating disease worldwide. Previously, we successfully generated canker-resistant Citrus sinensis cv. Hamlin lines in the T0 generation. This was achieved through the transformation of embryogenic protoplasts using the ribonucleoprotein (RNP) containing Cas12a and one crRNA to edit the canker susceptibility gene...

文献信息：Sarkar, Poulami, Vazquez, Jorge Santiago, Zhou, Mingxi, Levy, Amit, Mou, Zhonglin and Orbovic, Vladimir等.  请登录

简介：Several expression systems have been developed in clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) framework allowing for gene editing of disease-associated genes across diverse citrus varieties. In this study, we present a new approach employing a multi-intron containing Cas9 gene plus multiple gRNAs separated with tRNA sequences to target the ...

文献信息： Nunez-Munoz, Leandro Alberto, Sanchez-Garcia, Martin Eduardo, Calderon-Perez, Berenice, De la Torre-Almaraz, Rodolfo, Ruiz-Medrano, Roberto and Xocon等.  请登录

简介：Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tIssues. This study investigated the effects of genetically modified citrus trees expressing C...

文献信息：Mahmoud, Lamiaa M. and Dutt, Manjul等.  请登录

简介： Key messageA lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

文献信息：Liu, Hao, Deng, Bin, Chen, Feng-Hao, Suo, Jia-Qi, Ouyang, Ge-Cheng, Lu, Hui-Lin, Chen, Da-Song and Meng, Xiang等.  请登录

简介：BACKGROUND: Citrus huanglongbing (HLB) is a devastating disease caused by Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry. In nature, CLas relies primarily on Diaphorina citri Kuwayama as its vector for dissemination. After D. citri ingests CLas-infected citrus, the pathogen infiltrates the insect's body, where it thrives, reproduces, and exerts regulatory control over th...

文献信息：Li, Q., Xian, B., Yu, Q., Jia, R., Zhang, C., Zhong, X., Zhang, M., Fu, Y., Liu, Y., He, H., Li, M., Chen, S. and He, Y.等.  请登录

简介：Citrus bacterial canker (CBC) is a serious bacterial disease caused by Xanthomonas citri subsp. citri (Xcc) that adversely impacts the global citrus industry. In a previous study, we demonstrated that overexpression of an Xcc-inducible apetala 2/ethylene response factor encoded by Citrus sinensis, CsAP2-09, enhances CBC resistance. The mechanism responsible for this effect, however, is not known. ...

文献信息：Kishk, Abdelaziz, Stelinski, Lukasz L., Gowda, Siddarame and Killiny, Nabil等.  请登录

简介：BACKGROUND: Asian citrus psyllid, Diaphorina citri, is a hemipteran that vectors the causal pathogen of citrus greening disease, or huanglongbing (HLB). HLB is a tree killing disease that has severely limited citrus production globally. Unfortunately, there is no cure for this disease, and mitigation depends on multiple insecticide applications to reduce vector populations. Silencing of cytochrome...

文献信息：Jia, Hongge, Omar, Ahmad A., Xu, Jin, Dalmendray, Javier, Wang, Yuanchun, Feng, Yu, Wang, Wenting, Hu, Zhuyuan, Grosser, Jude W. and Wang, Nian等.  请登录

简介： Citrus canker disease affects citrus production. This disease is caused by Xanthomonas citri subsp. citri (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), is translocated from the pathogen to host plant cells. PthA4 binds to the effector binding elements (EBEs) in the promoter region of canker susceptibility gene LOB1 (EBEPthA4-...

文献信息：Huang, X., Su, L., Xian, B., Yu, Q., Zhang, M., Fan, J., Zhang, C., Liu, Y., He, H., Zhong, X., Li, M., Chen, S., He, Y. and Li, Q.等.  请登录

简介：Citrus bacterial canker (CBC) is a harmful bacterial disease caused by Xanthomonas citri subsp. citri (Xcc), negatively impacting citrus production worldwide. The basic helix-loop-helix (bHLH) transcription factor family plays crucial roles in plant development and stress responses. This study aimed to identify and annotate bHLH proteins encoded in the Citrus sinensis genome and explore their invo...

文献信息：Huang, Guiyan, Chang, Xiaopeng, Hu, Yanan, Li, Fuxuan, Wang, Nian and Li, Ruimin等.  请登录

简介：Citrus huanglongbing (HLB), which is caused by the phloem-colonizing bacteria Candidatus Liberibacter asiaticus (CLas), poses a significant threat to citrus production worldwide. The pathogenicity mechanism of HLB remains poorly understood. SEC-dependent effectors (SDEs) have been suggested to play critical roles in the interaction between citrus and CLas. Here, we explored the function of CLIBASI...

文献信息：Gill, Rafaqat A., Li, Xianglian, Duan, Shuo, Xing, Qian and Mueller-Xing, Ralf等.  请登录

文献信息：Fan, J., Xian, B., Huang, X., Yu, Q., Zhang, M., Zhang, C., Jia, R., Chen, S., He, Y. and Li, Q.等.  2024-03-13 请登录

简介：Citrus bacterial canker (CBC) is a severe bacterial infection caused by Xanthomonas citri subsp. citri (Xcc), which continues to adversely impact citrus production worldwide. Members of the GATA family are important regulators of plant development and regulate plant responses to particular stressors. This report aimed to systematically elucidate the Citrus sinensis genome to identify and annotate ...

文献信息：Chhabra, Gautam, Sharma, Manveer, Kalia, Anu, Kaur, Ajinder and Sandhu, Jagdeep Singh等.  请登录

简介：Citrus spp. are recalcitrant to in vitro shoot regeneration and we report an improved in planta protocol for genetic transformation of rough lemon that bypasses shoot regeneration in tIssue culture. The features of the protocol were the use of an Agrobacterium suspension with an OD600 nm = 0.6-1.0 supplemented with 100 mu g acetosyringone, gentle shaking of embryo axes pricked at shoot apical meri...

文献信息：Alessandra Alves de Souza; Mariana Bossi, Esteves等.  请登录

简介：Huanglongbing (HLB) is the main disease affecting sweet orange production worldwide, leading to significant losses in the sector. It is predominantly caused by the fastidious gram-negative ±-proteobacteria Candidatus Liberibacter asiaticus (CLas) and transmitted by the psyllid, Diaphorina citri. Notably, diffusible signal factor (DSF), a molecule essential to the quorum-sensing (QS) systems of va...

文献信息：张书舍 周常勇 and 赵廷昌等.  请登录

简介：柑橘黄龙病（Citrus Huanglongbing,HLB）是世界范围内柑橘生产上的毁灭性病害,已在50多个国家和地区发生流行,目前尚无抗（耐）柑橘品种可供商用。该病由韧皮部专性寄生的革兰氏阴性菌引起,分为亚洲种（Candidatus Liberibacter asiaticus,CLas）、非洲种（Ca.L.africanus,CLaf）和美洲种（Ca.L.americanus,CLam）,其中因CLas分布广、致病力强而被广泛研究。病原菌不可体外培养的特性制约了对HLB发病机制的研究,探究效应子功能则有助...

文献信息：彭爱红, 张婧芸, 陈志毅, 苏娟, 何永睿 and 姚利晓等.  请登录

简介：为了明确扩展蛋白基因CsEXPA8的生物学功能，通过构建超量表达和干扰表达CsEXPA8的载体转化易感溃疡病的柑橘品种‘晚锦橙’，对获得的转基因植株进行表型分析和对溃疡病抗性评价。结果表明，CsEXPA8在‘晚锦橙’中具有组织表达特异性，并受溃疡病菌诱导表达；CsEXPA8的过表达促进了叶片的长度和宽度的增加以及春梢直径的增粗，提高了对溃疡病的敏感性；而干扰CsEXPA8的表达后，转基因植株的叶片长度、宽度和春梢直径变小，但对溃疡病...

文献信息：Zhu Xie, Shuai Wang, Juanjuan Ma, Liting Dong, Rongrong Qu, Yong-Rui He, Shanchun Chen*, Xiuping Zou等.  请登录

文献信息：Zhang Yang, Xiao Wei, Wang Min,Khan Madiha,Liu Ji-Hong等.  请登录

简介：The Cys2/His2 (C2H2)-type zinc finger family has been reported to regulate multiple aspects of plant development and abiotic stress response. However, the role of C2H2-type zinc finger proteins in cold tolerance remains largely unclear. Through RNA-sequence analysis, a cold-responsive zinc finger protein, named as PtrZAT12, was identified and isolated from trifoliate orange (Poncirus trifoliata L....